3D-printed micro bubble column reactor with integrated microsensors for biotechnological applications: From design to evaluation

Abstract With the technological advances in 3D printing technology, which are associated with ever-increasing printing resolution, additive manufacturing is now increasingly being used for rapid manufacturing of complex devices including microsystems development for laboratory applications. Personal...

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Autores principales:	Lasse Jannis Frey, David Vorländer, Hendrik Ostsieker, Detlev Rasch, Jan-Luca Lohse, Maximilian Breitfeld, Jan-Hendrik Grosch, Gregor D. Wehinger, Janina Bahnemann, Rainer Krull
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d13c3984e82049009dfa236c2c49a2d8
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spelling	oai:doaj.org-article:d13c3984e82049009dfa236c2c49a2d82021-12-02T14:24:55Z3D-printed micro bubble column reactor with integrated microsensors for biotechnological applications: From design to evaluation10.1038/s41598-021-86654-92045-2322https://doaj.org/article/d13c3984e82049009dfa236c2c49a2d82021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86654-9https://doaj.org/toc/2045-2322Abstract With the technological advances in 3D printing technology, which are associated with ever-increasing printing resolution, additive manufacturing is now increasingly being used for rapid manufacturing of complex devices including microsystems development for laboratory applications. Personalized experimental devices or entire bioreactors of high complexity can be manufactured within few hours from start to finish. This study presents a customized 3D-printed micro bubble column reactor (3D-µBCR), which can be used for the cultivation of microorganisms (e.g., Saccharomyces cerevisiae) and allows online-monitoring of process parameters through integrated microsensor technology. The modular 3D-µBCR achieves rapid homogenization in less than 1 s and high oxygen transfer with k L a values up to 788 h−1 and is able to monitor biomass, pH, and DOT in the fluid phase, as well as CO2 and O2 in the gas phase. By extensive comparison of different reactor designs, the influence of the geometry on the resulting hydrodynamics was investigated. In order to quantify local flow patterns in the fluid, a three-dimensional and transient multiphase Computational Fluid Dynamics model was successfully developed and applied. The presented 3D-µBCR shows enormous potential for experimental parallelization and enables a high level of flexibility in reactor design, which can support versatile process development.Lasse Jannis FreyDavid VorländerHendrik OstsiekerDetlev RaschJan-Luca LohseMaximilian BreitfeldJan-Hendrik GroschGregor D. WehingerJanina BahnemannRainer KrullNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lasse Jannis Frey David Vorländer Hendrik Ostsieker Detlev Rasch Jan-Luca Lohse Maximilian Breitfeld Jan-Hendrik Grosch Gregor D. Wehinger Janina Bahnemann Rainer Krull 3D-printed micro bubble column reactor with integrated microsensors for biotechnological applications: From design to evaluation
description	Abstract With the technological advances in 3D printing technology, which are associated with ever-increasing printing resolution, additive manufacturing is now increasingly being used for rapid manufacturing of complex devices including microsystems development for laboratory applications. Personalized experimental devices or entire bioreactors of high complexity can be manufactured within few hours from start to finish. This study presents a customized 3D-printed micro bubble column reactor (3D-µBCR), which can be used for the cultivation of microorganisms (e.g., Saccharomyces cerevisiae) and allows online-monitoring of process parameters through integrated microsensor technology. The modular 3D-µBCR achieves rapid homogenization in less than 1 s and high oxygen transfer with k L a values up to 788 h−1 and is able to monitor biomass, pH, and DOT in the fluid phase, as well as CO2 and O2 in the gas phase. By extensive comparison of different reactor designs, the influence of the geometry on the resulting hydrodynamics was investigated. In order to quantify local flow patterns in the fluid, a three-dimensional and transient multiphase Computational Fluid Dynamics model was successfully developed and applied. The presented 3D-µBCR shows enormous potential for experimental parallelization and enables a high level of flexibility in reactor design, which can support versatile process development.
format	article
author	Lasse Jannis Frey David Vorländer Hendrik Ostsieker Detlev Rasch Jan-Luca Lohse Maximilian Breitfeld Jan-Hendrik Grosch Gregor D. Wehinger Janina Bahnemann Rainer Krull
author_facet	Lasse Jannis Frey David Vorländer Hendrik Ostsieker Detlev Rasch Jan-Luca Lohse Maximilian Breitfeld Jan-Hendrik Grosch Gregor D. Wehinger Janina Bahnemann Rainer Krull
author_sort	Lasse Jannis Frey
title	3D-printed micro bubble column reactor with integrated microsensors for biotechnological applications: From design to evaluation
title_short	3D-printed micro bubble column reactor with integrated microsensors for biotechnological applications: From design to evaluation
title_full	3D-printed micro bubble column reactor with integrated microsensors for biotechnological applications: From design to evaluation
title_fullStr	3D-printed micro bubble column reactor with integrated microsensors for biotechnological applications: From design to evaluation
title_full_unstemmed	3D-printed micro bubble column reactor with integrated microsensors for biotechnological applications: From design to evaluation
title_sort	3d-printed micro bubble column reactor with integrated microsensors for biotechnological applications: from design to evaluation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d13c3984e82049009dfa236c2c49a2d8
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3D-printed micro bubble column reactor with integrated microsensors for biotechnological applications: From design to evaluation

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