Analysis of Müller glia specific genes and their histone modification using Hes1-promoter driven EGFP expressing mouse

Abstract Retinal neurons and Müller glia are generated from a common population of multipotent retinal progenitor cells. We purposed to identify Müller glia-specific molecular signatures during retinal development. Using transgenic mice carrying the Hes1 promoter (pHes1) followed by EGFP, we purifie...

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Autores principales:	Kazuko Ueno, Toshiro Iwagawa, Genki Ochiai, Hideto Koso, Hiromitsu Nakauchi, Masao Nagasaki, Yutaka Suzuki, Sumiko Watanabe
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d13c75c763564fd2aede7e1fc76eaccc
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spelling	oai:doaj.org-article:d13c75c763564fd2aede7e1fc76eaccc2021-12-02T12:32:07ZAnalysis of Müller glia specific genes and their histone modification using Hes1-promoter driven EGFP expressing mouse10.1038/s41598-017-03874-82045-2322https://doaj.org/article/d13c75c763564fd2aede7e1fc76eaccc2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03874-8https://doaj.org/toc/2045-2322Abstract Retinal neurons and Müller glia are generated from a common population of multipotent retinal progenitor cells. We purposed to identify Müller glia-specific molecular signatures during retinal development. Using transgenic mice carrying the Hes1 promoter (pHes1) followed by EGFP, we purified EGFP-positive Müller glia and other EGFP-negative retinal cells from developing retinas and subjected them to RNA sequencing analysis. Gene expression pattern of EGFP-positive cell was similar to genes expressed in retinal progenitors, and they were downregulated in other cell lineages. Then, we examined the modification profiles of H3K27me3 and H3K4me3 by referring to chromatin immunoprecipitation-sequencing data of rods and other cells. Clustering of the H3K4me3 and H3K27me3 values followed by ontology analysis revealed a high incidence of transcription factors including Hes1 in clusters with high H3K27me3 levels. Hes1 expression level decreased dramatically, and the H3K27me3 level at the Hes1-locus was upregulated strongly during retinal development. Furthermore, the Hes1 expression level was upregulated in an Ezh2-knockout retina. These results suggest that downregulation of Müller glia-related genes in other lineage rather than upregulation of them in Müller glia contributed Müller-specific molecular features, and a role for modified H3K27me3 in suppressing Müller glia-related genes in other retinal cell lineages to avoid unfavorable expression.Kazuko UenoToshiro IwagawaGenki OchiaiHideto KosoHiromitsu NakauchiMasao NagasakiYutaka SuzukiSumiko WatanabeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kazuko Ueno Toshiro Iwagawa Genki Ochiai Hideto Koso Hiromitsu Nakauchi Masao Nagasaki Yutaka Suzuki Sumiko Watanabe Analysis of Müller glia specific genes and their histone modification using Hes1-promoter driven EGFP expressing mouse
description	Abstract Retinal neurons and Müller glia are generated from a common population of multipotent retinal progenitor cells. We purposed to identify Müller glia-specific molecular signatures during retinal development. Using transgenic mice carrying the Hes1 promoter (pHes1) followed by EGFP, we purified EGFP-positive Müller glia and other EGFP-negative retinal cells from developing retinas and subjected them to RNA sequencing analysis. Gene expression pattern of EGFP-positive cell was similar to genes expressed in retinal progenitors, and they were downregulated in other cell lineages. Then, we examined the modification profiles of H3K27me3 and H3K4me3 by referring to chromatin immunoprecipitation-sequencing data of rods and other cells. Clustering of the H3K4me3 and H3K27me3 values followed by ontology analysis revealed a high incidence of transcription factors including Hes1 in clusters with high H3K27me3 levels. Hes1 expression level decreased dramatically, and the H3K27me3 level at the Hes1-locus was upregulated strongly during retinal development. Furthermore, the Hes1 expression level was upregulated in an Ezh2-knockout retina. These results suggest that downregulation of Müller glia-related genes in other lineage rather than upregulation of them in Müller glia contributed Müller-specific molecular features, and a role for modified H3K27me3 in suppressing Müller glia-related genes in other retinal cell lineages to avoid unfavorable expression.
format	article
author	Kazuko Ueno Toshiro Iwagawa Genki Ochiai Hideto Koso Hiromitsu Nakauchi Masao Nagasaki Yutaka Suzuki Sumiko Watanabe
author_facet	Kazuko Ueno Toshiro Iwagawa Genki Ochiai Hideto Koso Hiromitsu Nakauchi Masao Nagasaki Yutaka Suzuki Sumiko Watanabe
author_sort	Kazuko Ueno
title	Analysis of Müller glia specific genes and their histone modification using Hes1-promoter driven EGFP expressing mouse
title_short	Analysis of Müller glia specific genes and their histone modification using Hes1-promoter driven EGFP expressing mouse
title_full	Analysis of Müller glia specific genes and their histone modification using Hes1-promoter driven EGFP expressing mouse
title_fullStr	Analysis of Müller glia specific genes and their histone modification using Hes1-promoter driven EGFP expressing mouse
title_full_unstemmed	Analysis of Müller glia specific genes and their histone modification using Hes1-promoter driven EGFP expressing mouse
title_sort	analysis of müller glia specific genes and their histone modification using hes1-promoter driven egfp expressing mouse
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d13c75c763564fd2aede7e1fc76eaccc
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Analysis of Müller glia specific genes and their histone modification using Hes1-promoter driven EGFP expressing mouse

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