Age-Related Changes in Plasma Extracellular Vesicle Characteristics and Internalization by Leukocytes

Abstract Cells release lipid-bound extracellular vesicles (EVs; exosomes, microvesicles and apoptotic bodies) containing proteins, lipids and RNAs into the circulation. Vesicles mediate intercellular communication between both neighboring and distant cells. There is substantial interest in using EVs...

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Autores principales:	Erez Eitan, Jamal Green, Monica Bodogai, Nicolle A. Mode, Rikke Bæk, Malene M. Jørgensen, David W. Freeman, Kenneth W. Witwer, Alan B. Zonderman, Arya Biragyn, Mark P. Mattson, Nicole Noren Hooten, Michele K. Evans
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d1409a205f414c7293502930c88f0308
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spelling	oai:doaj.org-article:d1409a205f414c7293502930c88f03082021-12-02T12:32:44ZAge-Related Changes in Plasma Extracellular Vesicle Characteristics and Internalization by Leukocytes10.1038/s41598-017-01386-z2045-2322https://doaj.org/article/d1409a205f414c7293502930c88f03082017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01386-zhttps://doaj.org/toc/2045-2322Abstract Cells release lipid-bound extracellular vesicles (EVs; exosomes, microvesicles and apoptotic bodies) containing proteins, lipids and RNAs into the circulation. Vesicles mediate intercellular communication between both neighboring and distant cells. There is substantial interest in using EVs as biomarkers for age-related diseases including cancer, and neurodegenerative, metabolic and cardiovascular diseases. The majority of research focuses on identifying differences in EVs when comparing disease states and matched controls. Here, we analyzed circulating plasma EVs in a cross-sectional and longitudinal study in order to address age-related changes in community-dwelling individuals. We found that EV concentration decreases with advancing age. Furthermore, EVs from older individuals were more readily internalized by B cells and increased MHC-II expression on monocytes compared with EVs from younger individuals, indicating that the decreased concentration of EVs with age may be due in part to increased internalization. EVs activated both monocytes and B cells, and activation of B cells by LPS enhanced EV internalization. We also report a relative stability of EV concentration and protein amount in individual subjects over time. Our data provide important information towards establishing a profile of EVs with human age, which will further aid in the development of EV-based diagnostics for aging and age-related diseases.Erez EitanJamal GreenMonica BodogaiNicolle A. ModeRikke BækMalene M. JørgensenDavid W. FreemanKenneth W. WitwerAlan B. ZondermanArya BiragynMark P. MattsonNicole Noren HootenMichele K. EvansNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Erez Eitan Jamal Green Monica Bodogai Nicolle A. Mode Rikke Bæk Malene M. Jørgensen David W. Freeman Kenneth W. Witwer Alan B. Zonderman Arya Biragyn Mark P. Mattson Nicole Noren Hooten Michele K. Evans Age-Related Changes in Plasma Extracellular Vesicle Characteristics and Internalization by Leukocytes
description	Abstract Cells release lipid-bound extracellular vesicles (EVs; exosomes, microvesicles and apoptotic bodies) containing proteins, lipids and RNAs into the circulation. Vesicles mediate intercellular communication between both neighboring and distant cells. There is substantial interest in using EVs as biomarkers for age-related diseases including cancer, and neurodegenerative, metabolic and cardiovascular diseases. The majority of research focuses on identifying differences in EVs when comparing disease states and matched controls. Here, we analyzed circulating plasma EVs in a cross-sectional and longitudinal study in order to address age-related changes in community-dwelling individuals. We found that EV concentration decreases with advancing age. Furthermore, EVs from older individuals were more readily internalized by B cells and increased MHC-II expression on monocytes compared with EVs from younger individuals, indicating that the decreased concentration of EVs with age may be due in part to increased internalization. EVs activated both monocytes and B cells, and activation of B cells by LPS enhanced EV internalization. We also report a relative stability of EV concentration and protein amount in individual subjects over time. Our data provide important information towards establishing a profile of EVs with human age, which will further aid in the development of EV-based diagnostics for aging and age-related diseases.
format	article
author	Erez Eitan Jamal Green Monica Bodogai Nicolle A. Mode Rikke Bæk Malene M. Jørgensen David W. Freeman Kenneth W. Witwer Alan B. Zonderman Arya Biragyn Mark P. Mattson Nicole Noren Hooten Michele K. Evans
author_facet	Erez Eitan Jamal Green Monica Bodogai Nicolle A. Mode Rikke Bæk Malene M. Jørgensen David W. Freeman Kenneth W. Witwer Alan B. Zonderman Arya Biragyn Mark P. Mattson Nicole Noren Hooten Michele K. Evans
author_sort	Erez Eitan
title	Age-Related Changes in Plasma Extracellular Vesicle Characteristics and Internalization by Leukocytes
title_short	Age-Related Changes in Plasma Extracellular Vesicle Characteristics and Internalization by Leukocytes
title_full	Age-Related Changes in Plasma Extracellular Vesicle Characteristics and Internalization by Leukocytes
title_fullStr	Age-Related Changes in Plasma Extracellular Vesicle Characteristics and Internalization by Leukocytes
title_full_unstemmed	Age-Related Changes in Plasma Extracellular Vesicle Characteristics and Internalization by Leukocytes
title_sort	age-related changes in plasma extracellular vesicle characteristics and internalization by leukocytes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d1409a205f414c7293502930c88f0308
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Age-Related Changes in Plasma Extracellular Vesicle Characteristics and Internalization by Leukocytes

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