Use of mutagenesis, genetic mapping and next generation transcriptomics to investigate insecticide resistance mechanisms.

Insecticide resistance is a worldwide problem with major impact on agriculture and human health. Understanding the underlying molecular mechanisms is crucial for the management of the phenomenon; however, this information often comes late with respect to the implementation of efficient counter-measu...

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Autores principales:	Predrag Kalajdzic, Stefan Oehler, Martin Reczko, Nena Pavlidi, John Vontas, Artemis G Hatzigeorgiou, Charalambos Savakis
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d146d350a5d741ea9ec8d252fe594df1
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spelling	oai:doaj.org-article:d146d350a5d741ea9ec8d252fe594df12021-11-18T07:13:38ZUse of mutagenesis, genetic mapping and next generation transcriptomics to investigate insecticide resistance mechanisms.1932-620310.1371/journal.pone.0040296https://doaj.org/article/d146d350a5d741ea9ec8d252fe594df12012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22768270/?tool=EBIhttps://doaj.org/toc/1932-6203Insecticide resistance is a worldwide problem with major impact on agriculture and human health. Understanding the underlying molecular mechanisms is crucial for the management of the phenomenon; however, this information often comes late with respect to the implementation of efficient counter-measures, particularly in the case of metabolism-based resistance mechanisms. We employed a genome-wide insertional mutagenesis screen to Drosophila melanogaster, using a Minos-based construct, and retrieved a line (MiT[w(-)]3R2) resistant to the neonicotinoid insecticide Imidacloprid. Biochemical and bioassay data indicated that resistance was due to increased P450 detoxification. Deep sequencing transcriptomic analysis revealed substantial over- and under-representation of 357 transcripts in the resistant line, including statistically significant changes in mixed function oxidases, peptidases and cuticular proteins. Three P450 genes (Cyp4p2, Cyp6a2 and Cyp6g1) located on the 2R chromosome, are highly up-regulated in mutant flies compared to susceptible Drosophila. One of them (Cyp6g1) has been already described as a major factor for Imidacloprid resistance, which validated the approach. Elevated expression of the Cyp4p2 was not previously documented in Drosophila lines resistant to neonicotinoids. In silico analysis using the Drosophila reference genome failed to detect transcription binding factors or microRNAs associated with the over-expressed Cyp genes. The resistant line did not contain a Minos insertion in its chromosomes, suggesting a hit-and-run event, i.e. an insertion of the transposable element, followed by an excision which caused the mutation. Genetic mapping placed the resistance locus to the right arm of the second chromosome, within a ∼1 Mb region, where the highly up-regulated Cyp6g1 gene is located. The nature of the unknown mutation that causes resistance is discussed on the basis of these results.Predrag KalajdzicStefan OehlerMartin ReczkoNena PavlidiJohn VontasArtemis G HatzigeorgiouCharalambos SavakisPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e40296 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Predrag Kalajdzic Stefan Oehler Martin Reczko Nena Pavlidi John Vontas Artemis G Hatzigeorgiou Charalambos Savakis Use of mutagenesis, genetic mapping and next generation transcriptomics to investigate insecticide resistance mechanisms.
description	Insecticide resistance is a worldwide problem with major impact on agriculture and human health. Understanding the underlying molecular mechanisms is crucial for the management of the phenomenon; however, this information often comes late with respect to the implementation of efficient counter-measures, particularly in the case of metabolism-based resistance mechanisms. We employed a genome-wide insertional mutagenesis screen to Drosophila melanogaster, using a Minos-based construct, and retrieved a line (MiT[w(-)]3R2) resistant to the neonicotinoid insecticide Imidacloprid. Biochemical and bioassay data indicated that resistance was due to increased P450 detoxification. Deep sequencing transcriptomic analysis revealed substantial over- and under-representation of 357 transcripts in the resistant line, including statistically significant changes in mixed function oxidases, peptidases and cuticular proteins. Three P450 genes (Cyp4p2, Cyp6a2 and Cyp6g1) located on the 2R chromosome, are highly up-regulated in mutant flies compared to susceptible Drosophila. One of them (Cyp6g1) has been already described as a major factor for Imidacloprid resistance, which validated the approach. Elevated expression of the Cyp4p2 was not previously documented in Drosophila lines resistant to neonicotinoids. In silico analysis using the Drosophila reference genome failed to detect transcription binding factors or microRNAs associated with the over-expressed Cyp genes. The resistant line did not contain a Minos insertion in its chromosomes, suggesting a hit-and-run event, i.e. an insertion of the transposable element, followed by an excision which caused the mutation. Genetic mapping placed the resistance locus to the right arm of the second chromosome, within a ∼1 Mb region, where the highly up-regulated Cyp6g1 gene is located. The nature of the unknown mutation that causes resistance is discussed on the basis of these results.
format	article
author	Predrag Kalajdzic Stefan Oehler Martin Reczko Nena Pavlidi John Vontas Artemis G Hatzigeorgiou Charalambos Savakis
author_facet	Predrag Kalajdzic Stefan Oehler Martin Reczko Nena Pavlidi John Vontas Artemis G Hatzigeorgiou Charalambos Savakis
author_sort	Predrag Kalajdzic
title	Use of mutagenesis, genetic mapping and next generation transcriptomics to investigate insecticide resistance mechanisms.
title_short	Use of mutagenesis, genetic mapping and next generation transcriptomics to investigate insecticide resistance mechanisms.
title_full	Use of mutagenesis, genetic mapping and next generation transcriptomics to investigate insecticide resistance mechanisms.
title_fullStr	Use of mutagenesis, genetic mapping and next generation transcriptomics to investigate insecticide resistance mechanisms.
title_full_unstemmed	Use of mutagenesis, genetic mapping and next generation transcriptomics to investigate insecticide resistance mechanisms.
title_sort	use of mutagenesis, genetic mapping and next generation transcriptomics to investigate insecticide resistance mechanisms.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/d146d350a5d741ea9ec8d252fe594df1
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Use of mutagenesis, genetic mapping and next generation transcriptomics to investigate insecticide resistance mechanisms.

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