Micropropagation of Three Endemic Begonias Using Various Hormones Concentration and Culture Media Application

Three species of Begonias endemic to Java and Sumatra, namely Begonia leuserensis, Begonia atricha and Begonia scottii, were conserved in Cibodas Botanic Gardens as sources of germplasm for ornamental plant and/or medicines. However, the information on efficient hormones concentration and their cult...

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Autores principales:	Lily Ismaini, Intani Quarta Lailaty, Muhammad Efendi
Formato:	article
Lenguaje:	EN ID
Publicado:	UIN Sunan Gunung Djati 2021
Materias:	begonia atricha, begonia leuserensis, endemic, micropropagation, plant conservation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d14a4e2e674b4384885048e68aa4a9f9
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spelling	oai:doaj.org-article:d14a4e2e674b4384885048e68aa4a9f92021-11-30T11:51:07ZMicropropagation of Three Endemic Begonias Using Various Hormones Concentration and Culture Media Application2541-42082548-160610.15575/biodjati.v6i2.13769https://doaj.org/article/d14a4e2e674b4384885048e68aa4a9f92021-11-01T00:00:00Zhttps://journal.uinsgd.ac.id/index.php/biodjati/article/view/13769https://doaj.org/toc/2541-4208https://doaj.org/toc/2548-1606Three species of Begonias endemic to Java and Sumatra, namely Begonia leuserensis, Begonia atricha and Begonia scottii, were conserved in Cibodas Botanic Gardens as sources of germplasm for ornamental plant and/or medicines. However, the information on efficient hormones concentration and their culture media application through an in vitro propagation effort is still limited. Therefore, this study aimed to explain the growth response of three species of Begonias using various hormones concentrations and culture media through in vitro propagation. The culture media using Murashige & Skoog (MS) media that combinedwith 6-Benzyladenine (BA) dan Thidiazuron (TDZ) hormones in different concentrations i.e. 0.5 mg/L, 1 mg/L, 2 mg/L, and 3 mg/L. Observation parameter included shoot number, plantlets height, and leaves number. The data were analyzed using analysis of variance (ANOVA) with the F test at a 5% significance level. The results showed that three species of Begonias were observed to have different growth responses in the combination of MS+BA and MS+TDZ media. The combination of MS+TDZ media produces more shoots number, while the combination of MS+BA media influenced higher in leaves number. A concentration of 0.5 mg/L of hormone showed a good regeneration, therefore were recommended for in vitro propagation of Begonia species.Lily IsmainiIntani Quarta LailatyMuhammad EfendiUIN Sunan Gunung Djatiarticlebegonia atricha, begonia leuserensis, endemic, micropropagation, plant conservationBiology (General)QH301-705.5ENIDJurnal Biodjati, Vol 6, Iss 2, Pp 284-294 (2021)
institution	DOAJ
collection	DOAJ
language	EN ID
topic	begonia atricha, begonia leuserensis, endemic, micropropagation, plant conservation Biology (General) QH301-705.5
spellingShingle	begonia atricha, begonia leuserensis, endemic, micropropagation, plant conservation Biology (General) QH301-705.5 Lily Ismaini Intani Quarta Lailaty Muhammad Efendi Micropropagation of Three Endemic Begonias Using Various Hormones Concentration and Culture Media Application
description	Three species of Begonias endemic to Java and Sumatra, namely Begonia leuserensis, Begonia atricha and Begonia scottii, were conserved in Cibodas Botanic Gardens as sources of germplasm for ornamental plant and/or medicines. However, the information on efficient hormones concentration and their culture media application through an in vitro propagation effort is still limited. Therefore, this study aimed to explain the growth response of three species of Begonias using various hormones concentrations and culture media through in vitro propagation. The culture media using Murashige & Skoog (MS) media that combinedwith 6-Benzyladenine (BA) dan Thidiazuron (TDZ) hormones in different concentrations i.e. 0.5 mg/L, 1 mg/L, 2 mg/L, and 3 mg/L. Observation parameter included shoot number, plantlets height, and leaves number. The data were analyzed using analysis of variance (ANOVA) with the F test at a 5% significance level. The results showed that three species of Begonias were observed to have different growth responses in the combination of MS+BA and MS+TDZ media. The combination of MS+TDZ media produces more shoots number, while the combination of MS+BA media influenced higher in leaves number. A concentration of 0.5 mg/L of hormone showed a good regeneration, therefore were recommended for in vitro propagation of Begonia species.
format	article
author	Lily Ismaini Intani Quarta Lailaty Muhammad Efendi
author_facet	Lily Ismaini Intani Quarta Lailaty Muhammad Efendi
author_sort	Lily Ismaini
title	Micropropagation of Three Endemic Begonias Using Various Hormones Concentration and Culture Media Application
title_short	Micropropagation of Three Endemic Begonias Using Various Hormones Concentration and Culture Media Application
title_full	Micropropagation of Three Endemic Begonias Using Various Hormones Concentration and Culture Media Application
title_fullStr	Micropropagation of Three Endemic Begonias Using Various Hormones Concentration and Culture Media Application
title_full_unstemmed	Micropropagation of Three Endemic Begonias Using Various Hormones Concentration and Culture Media Application
title_sort	micropropagation of three endemic begonias using various hormones concentration and culture media application
publisher	UIN Sunan Gunung Djati
publishDate	2021
url	https://doaj.org/article/d14a4e2e674b4384885048e68aa4a9f9
work_keys_str_mv	AT lilyismaini micropropagationofthreeendemicbegoniasusingvarioushormonesconcentrationandculturemediaapplication AT intaniquartalailaty micropropagationofthreeendemicbegoniasusingvarioushormonesconcentrationandculturemediaapplication AT muhammadefendi micropropagationofthreeendemicbegoniasusingvarioushormonesconcentrationandculturemediaapplication
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Micropropagation of Three Endemic Begonias Using Various Hormones Concentration and Culture Media Application

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