BIOLOGICAL EFFECTS OF TNF-BINDING VARIOLAVIRUS RECOMBINANT PROTEIN

Abstract. This review presents a summary of our data concerning in vivo and in vitro effects of recombinant TNF-binding protein from variola virus (VARVCrmB) upon TNF-induced functional changes of human and murine cells. VARV-CrmB protein blocks TNF-induced production of IL-1β and IL-6 by human mono...

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Autores principales:	I. A. Orlovskaya, D. D. Tsyrendorzhiev, L. B. Toporkova, V. V. Kurilin, Y. A. Lopatnikova, E. A. Vyazovaya, I. P. Gileva, S. N. Schelkunov, S. V. Sennikov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	tnf tnf-binding protein contact dermatitis collagen-induced arthritis Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/d14b70b0f2b74107a38287519f059cc7
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spelling	oai:doaj.org-article:d14b70b0f2b74107a38287519f059cc72021-11-18T08:03:39ZBIOLOGICAL EFFECTS OF TNF-BINDING VARIOLAVIRUS RECOMBINANT PROTEIN1563-06252313-741X10.15789/1563-0625-2012-1-2-33-42https://doaj.org/article/d14b70b0f2b74107a38287519f059cc72014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/292https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. This review presents a summary of our data concerning in vivo and in vitro effects of recombinant TNF-binding protein from variola virus (VARVCrmB) upon TNF-induced functional changes of human and murine cells. VARV-CrmB protein blocks TNF-induced production of IL-1β and IL-6 by human mononuclear cells, and their in vitro oxidation-related metabolic (OM) activity. VARV-CrmB protein restores TNF-induced reduction of BFU-E+CFU-E colony-forming activity and normalizes TNF-induced effects upon CFU-GM formation in a colony-forming model of human and murine bone morrow cells (BMC). VARV-CrmB protein displays a pronounced in vivo alleviation of LPS-induced endotoxic shock symptoms in SPF BALB/C mice thus significantly increasing survival of experimental animals. Moreover, VARV-CrmBprotein decreases intensity of collagen-induced arthritis at early terms. Application of VARV-CrmB protein results in normalization of TNF-induced increase of migratory and OM activity of murine leukocytes, and exerts corrective effects upon colony-forming ability of murine hematopoietic precursors. Skin application of VARV-CrmB protein decreases leukocyte migration from a skin scrap in afferent phase of DNCB-induced contact reaction, as well as “ear oedema” index. Our results demonstrate TNF-blocking properties of VARVCrmB protein. In summary, our data allow to consider a recombinant variola virus VARV-CrmB as a new potential TNF-antagonist. Its effects can be explained by its ability of neutralizing TNF-induced activation of oxidation-related metabolic, cytokine-producing and migratory functions of effector cells in therapy of pathological inflammatory processes.I. A. OrlovskayaD. D. TsyrendorzhievL. B. ToporkovaV. V. KurilinY. A. LopatnikovaE. A. VyazovayaI. P. GilevaS. N. SchelkunovS. V. SennikovSPb RAACIarticletnftnf-binding proteincontact dermatitiscollagen-induced arthritisImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 14, Iss 1-2, Pp 33-42 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	tnf tnf-binding protein contact dermatitis collagen-induced arthritis Immunologic diseases. Allergy RC581-607
spellingShingle	tnf tnf-binding protein contact dermatitis collagen-induced arthritis Immunologic diseases. Allergy RC581-607 I. A. Orlovskaya D. D. Tsyrendorzhiev L. B. Toporkova V. V. Kurilin Y. A. Lopatnikova E. A. Vyazovaya I. P. Gileva S. N. Schelkunov S. V. Sennikov BIOLOGICAL EFFECTS OF TNF-BINDING VARIOLAVIRUS RECOMBINANT PROTEIN
description	Abstract. This review presents a summary of our data concerning in vivo and in vitro effects of recombinant TNF-binding protein from variola virus (VARVCrmB) upon TNF-induced functional changes of human and murine cells. VARV-CrmB protein blocks TNF-induced production of IL-1β and IL-6 by human mononuclear cells, and their in vitro oxidation-related metabolic (OM) activity. VARV-CrmB protein restores TNF-induced reduction of BFU-E+CFU-E colony-forming activity and normalizes TNF-induced effects upon CFU-GM formation in a colony-forming model of human and murine bone morrow cells (BMC). VARV-CrmB protein displays a pronounced in vivo alleviation of LPS-induced endotoxic shock symptoms in SPF BALB/C mice thus significantly increasing survival of experimental animals. Moreover, VARV-CrmBprotein decreases intensity of collagen-induced arthritis at early terms. Application of VARV-CrmB protein results in normalization of TNF-induced increase of migratory and OM activity of murine leukocytes, and exerts corrective effects upon colony-forming ability of murine hematopoietic precursors. Skin application of VARV-CrmB protein decreases leukocyte migration from a skin scrap in afferent phase of DNCB-induced contact reaction, as well as “ear oedema” index. Our results demonstrate TNF-blocking properties of VARVCrmB protein. In summary, our data allow to consider a recombinant variola virus VARV-CrmB as a new potential TNF-antagonist. Its effects can be explained by its ability of neutralizing TNF-induced activation of oxidation-related metabolic, cytokine-producing and migratory functions of effector cells in therapy of pathological inflammatory processes.
format	article
author	I. A. Orlovskaya D. D. Tsyrendorzhiev L. B. Toporkova V. V. Kurilin Y. A. Lopatnikova E. A. Vyazovaya I. P. Gileva S. N. Schelkunov S. V. Sennikov
author_facet	I. A. Orlovskaya D. D. Tsyrendorzhiev L. B. Toporkova V. V. Kurilin Y. A. Lopatnikova E. A. Vyazovaya I. P. Gileva S. N. Schelkunov S. V. Sennikov
author_sort	I. A. Orlovskaya
title	BIOLOGICAL EFFECTS OF TNF-BINDING VARIOLAVIRUS RECOMBINANT PROTEIN
title_short	BIOLOGICAL EFFECTS OF TNF-BINDING VARIOLAVIRUS RECOMBINANT PROTEIN
title_full	BIOLOGICAL EFFECTS OF TNF-BINDING VARIOLAVIRUS RECOMBINANT PROTEIN
title_fullStr	BIOLOGICAL EFFECTS OF TNF-BINDING VARIOLAVIRUS RECOMBINANT PROTEIN
title_full_unstemmed	BIOLOGICAL EFFECTS OF TNF-BINDING VARIOLAVIRUS RECOMBINANT PROTEIN
title_sort	biological effects of tnf-binding variolavirus recombinant protein
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/d14b70b0f2b74107a38287519f059cc7
work_keys_str_mv	AT iaorlovskaya biologicaleffectsoftnfbindingvariolavirusrecombinantprotein AT ddtsyrendorzhiev biologicaleffectsoftnfbindingvariolavirusrecombinantprotein AT lbtoporkova biologicaleffectsoftnfbindingvariolavirusrecombinantprotein AT vvkurilin biologicaleffectsoftnfbindingvariolavirusrecombinantprotein AT yalopatnikova biologicaleffectsoftnfbindingvariolavirusrecombinantprotein AT eavyazovaya biologicaleffectsoftnfbindingvariolavirusrecombinantprotein AT ipgileva biologicaleffectsoftnfbindingvariolavirusrecombinantprotein AT snschelkunov biologicaleffectsoftnfbindingvariolavirusrecombinantprotein AT svsennikov biologicaleffectsoftnfbindingvariolavirusrecombinantprotein
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BIOLOGICAL EFFECTS OF TNF-BINDING VARIOLAVIRUS RECOMBINANT PROTEIN

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