Rapid on-site monitoring of Legionella pneumophila in cooling tower water using a portable microfluidic system

Abstract Legionnaires’ disease, predominantly caused by the bacterium Legionella pneumophila, has increased in prevalence worldwide. The most common mode of transmission of Legionella is inhalation of contaminated aerosols, such as those generated by cooling towers. Simple, rapid and accurate method...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nobuyasu Yamaguchi, Yusuke Tokunaga, Satoko Goto, Yudai Fujii, Fumiya Banno, Akiko Edagawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d14c957a482440a3b6f5e94c6876b062
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d14c957a482440a3b6f5e94c6876b062
record_format	dspace
spelling	oai:doaj.org-article:d14c957a482440a3b6f5e94c6876b0622021-12-02T11:52:37ZRapid on-site monitoring of Legionella pneumophila in cooling tower water using a portable microfluidic system10.1038/s41598-017-03293-92045-2322https://doaj.org/article/d14c957a482440a3b6f5e94c6876b0622017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03293-9https://doaj.org/toc/2045-2322Abstract Legionnaires’ disease, predominantly caused by the bacterium Legionella pneumophila, has increased in prevalence worldwide. The most common mode of transmission of Legionella is inhalation of contaminated aerosols, such as those generated by cooling towers. Simple, rapid and accurate methods to enumerate L. pneumophila are required to prevent the spread of this organism. Here, we applied a microfluidic device for on-chip fluorescent staining and semi-automated counting of L. pneumophila in cooling tower water. We also constructed a portable system for rapid on-site monitoring and used it to enumerate target bacterial cells rapidly flowing in the microchannel. A fluorescently-labelled polyclonal antibody was used for the selective detection of L. pneumophila serogroup 1 in the samples. The counts of L. pneumophila in cooling tower water obtained using the system and fluorescence microscopy were similar. The detection limit of the system was 104 cells/ml, but lower numbers of L. pneumophila cells (101 to 103 cells/ml) could be detected following concentration of 0.5–3 L of the water sample by filtration. Our technique is rapid to perform (1.5 h), semi-automated (on-chip staining and counting), and portable for on-site measurement, and it may therefore be effective in the initial screening of Legionella contamination in freshwater.Nobuyasu YamaguchiYusuke TokunagaSatoko GotoYudai FujiiFumiya BannoAkiko EdagawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nobuyasu Yamaguchi Yusuke Tokunaga Satoko Goto Yudai Fujii Fumiya Banno Akiko Edagawa Rapid on-site monitoring of Legionella pneumophila in cooling tower water using a portable microfluidic system
description	Abstract Legionnaires’ disease, predominantly caused by the bacterium Legionella pneumophila, has increased in prevalence worldwide. The most common mode of transmission of Legionella is inhalation of contaminated aerosols, such as those generated by cooling towers. Simple, rapid and accurate methods to enumerate L. pneumophila are required to prevent the spread of this organism. Here, we applied a microfluidic device for on-chip fluorescent staining and semi-automated counting of L. pneumophila in cooling tower water. We also constructed a portable system for rapid on-site monitoring and used it to enumerate target bacterial cells rapidly flowing in the microchannel. A fluorescently-labelled polyclonal antibody was used for the selective detection of L. pneumophila serogroup 1 in the samples. The counts of L. pneumophila in cooling tower water obtained using the system and fluorescence microscopy were similar. The detection limit of the system was 104 cells/ml, but lower numbers of L. pneumophila cells (101 to 103 cells/ml) could be detected following concentration of 0.5–3 L of the water sample by filtration. Our technique is rapid to perform (1.5 h), semi-automated (on-chip staining and counting), and portable for on-site measurement, and it may therefore be effective in the initial screening of Legionella contamination in freshwater.
format	article
author	Nobuyasu Yamaguchi Yusuke Tokunaga Satoko Goto Yudai Fujii Fumiya Banno Akiko Edagawa
author_facet	Nobuyasu Yamaguchi Yusuke Tokunaga Satoko Goto Yudai Fujii Fumiya Banno Akiko Edagawa
author_sort	Nobuyasu Yamaguchi
title	Rapid on-site monitoring of Legionella pneumophila in cooling tower water using a portable microfluidic system
title_short	Rapid on-site monitoring of Legionella pneumophila in cooling tower water using a portable microfluidic system
title_full	Rapid on-site monitoring of Legionella pneumophila in cooling tower water using a portable microfluidic system
title_fullStr	Rapid on-site monitoring of Legionella pneumophila in cooling tower water using a portable microfluidic system
title_full_unstemmed	Rapid on-site monitoring of Legionella pneumophila in cooling tower water using a portable microfluidic system
title_sort	rapid on-site monitoring of legionella pneumophila in cooling tower water using a portable microfluidic system
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d14c957a482440a3b6f5e94c6876b062
work_keys_str_mv	AT nobuyasuyamaguchi rapidonsitemonitoringoflegionellapneumophilaincoolingtowerwaterusingaportablemicrofluidicsystem AT yusuketokunaga rapidonsitemonitoringoflegionellapneumophilaincoolingtowerwaterusingaportablemicrofluidicsystem AT satokogoto rapidonsitemonitoringoflegionellapneumophilaincoolingtowerwaterusingaportablemicrofluidicsystem AT yudaifujii rapidonsitemonitoringoflegionellapneumophilaincoolingtowerwaterusingaportablemicrofluidicsystem AT fumiyabanno rapidonsitemonitoringoflegionellapneumophilaincoolingtowerwaterusingaportablemicrofluidicsystem AT akikoedagawa rapidonsitemonitoringoflegionellapneumophilaincoolingtowerwaterusingaportablemicrofluidicsystem
_version_	1718395012433051648

Rapid on-site monitoring of Legionella pneumophila in cooling tower water using a portable microfluidic system

Ejemplares similares