Correlation between Biophysical Properties of Niosomes Elaborated with Chloroquine and Different Tensioactives and Their Transfection Efficiency

Lipid nanocarriers, such as niosomes, are considered attractive candidates for non-viral gene delivery due to their suitable biocompatibility and high versatility. In this work, we studied the influence of incorporating chloroquine in niosomes biophysical performance, as well as the effect of non-io...

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Autores principales:	Myriam Sainz-Ramos, Ilia Villate-Beitia, Idoia Gallego, Nuseibah AL Qtaish, Margarita Menéndez, Laura Lagartera, Santiago Grijalvo, Ramón Eritja, Gustavo Puras, José Luis Pedraz
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	chloroquine niosomes gene delivery biophysical properties surfactants Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/d14fa7f0890244419ea891e2e19ba7f5
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spelling	oai:doaj.org-article:d14fa7f0890244419ea891e2e19ba7f52021-11-25T18:40:42ZCorrelation between Biophysical Properties of Niosomes Elaborated with Chloroquine and Different Tensioactives and Their Transfection Efficiency10.3390/pharmaceutics131117871999-4923https://doaj.org/article/d14fa7f0890244419ea891e2e19ba7f52021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1787https://doaj.org/toc/1999-4923Lipid nanocarriers, such as niosomes, are considered attractive candidates for non-viral gene delivery due to their suitable biocompatibility and high versatility. In this work, we studied the influence of incorporating chloroquine in niosomes biophysical performance, as well as the effect of non-ionic surfactant composition and protocol of incorporation in their biophysical performance. An exhaustive comparative evaluation of three niosome formulations differing in these parameters was performed, which included the analysis of their thermal stability, rheological behavior, mean particle size, dispersity, zeta potential, morphology, membrane packing capacity, affinity to bind DNA, ability to release and protect the genetic material, buffering capacity and ability to escape from artificially synthesized lysosomes. Finally, in vitro biological studies were, also, performed in order to determine the compatibility of the formulations with biological systems, their transfection efficiency and transgene expression. Results revealed that the incorporation of chloroquine in niosome formulations improved their biophysical properties and the transfection efficiency, while the substitution of one of the non-ionic surfactants and the phase of addition resulted in less biophysical variations. Of note, the present work provides several biophysical parameters and characterization strategies that could be used as gold standard for gene therapy nanosystems evaluation.Myriam Sainz-RamosIlia Villate-BeitiaIdoia GallegoNuseibah AL QtaishMargarita MenéndezLaura LagarteraSantiago GrijalvoRamón EritjaGustavo PurasJosé Luis PedrazMDPI AGarticlechloroquineniosomesgene deliverybiophysical propertiessurfactantsPharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1787, p 1787 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chloroquine niosomes gene delivery biophysical properties surfactants Pharmacy and materia medica RS1-441
spellingShingle	chloroquine niosomes gene delivery biophysical properties surfactants Pharmacy and materia medica RS1-441 Myriam Sainz-Ramos Ilia Villate-Beitia Idoia Gallego Nuseibah AL Qtaish Margarita Menéndez Laura Lagartera Santiago Grijalvo Ramón Eritja Gustavo Puras José Luis Pedraz Correlation between Biophysical Properties of Niosomes Elaborated with Chloroquine and Different Tensioactives and Their Transfection Efficiency
description	Lipid nanocarriers, such as niosomes, are considered attractive candidates for non-viral gene delivery due to their suitable biocompatibility and high versatility. In this work, we studied the influence of incorporating chloroquine in niosomes biophysical performance, as well as the effect of non-ionic surfactant composition and protocol of incorporation in their biophysical performance. An exhaustive comparative evaluation of three niosome formulations differing in these parameters was performed, which included the analysis of their thermal stability, rheological behavior, mean particle size, dispersity, zeta potential, morphology, membrane packing capacity, affinity to bind DNA, ability to release and protect the genetic material, buffering capacity and ability to escape from artificially synthesized lysosomes. Finally, in vitro biological studies were, also, performed in order to determine the compatibility of the formulations with biological systems, their transfection efficiency and transgene expression. Results revealed that the incorporation of chloroquine in niosome formulations improved their biophysical properties and the transfection efficiency, while the substitution of one of the non-ionic surfactants and the phase of addition resulted in less biophysical variations. Of note, the present work provides several biophysical parameters and characterization strategies that could be used as gold standard for gene therapy nanosystems evaluation.
format	article
author	Myriam Sainz-Ramos Ilia Villate-Beitia Idoia Gallego Nuseibah AL Qtaish Margarita Menéndez Laura Lagartera Santiago Grijalvo Ramón Eritja Gustavo Puras José Luis Pedraz
author_facet	Myriam Sainz-Ramos Ilia Villate-Beitia Idoia Gallego Nuseibah AL Qtaish Margarita Menéndez Laura Lagartera Santiago Grijalvo Ramón Eritja Gustavo Puras José Luis Pedraz
author_sort	Myriam Sainz-Ramos
title	Correlation between Biophysical Properties of Niosomes Elaborated with Chloroquine and Different Tensioactives and Their Transfection Efficiency
title_short	Correlation between Biophysical Properties of Niosomes Elaborated with Chloroquine and Different Tensioactives and Their Transfection Efficiency
title_full	Correlation between Biophysical Properties of Niosomes Elaborated with Chloroquine and Different Tensioactives and Their Transfection Efficiency
title_fullStr	Correlation between Biophysical Properties of Niosomes Elaborated with Chloroquine and Different Tensioactives and Their Transfection Efficiency
title_full_unstemmed	Correlation between Biophysical Properties of Niosomes Elaborated with Chloroquine and Different Tensioactives and Their Transfection Efficiency
title_sort	correlation between biophysical properties of niosomes elaborated with chloroquine and different tensioactives and their transfection efficiency
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d14fa7f0890244419ea891e2e19ba7f5
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Correlation between Biophysical Properties of Niosomes Elaborated with Chloroquine and Different Tensioactives and Their Transfection Efficiency

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