FTIR, Raman and AFM characterization of the clinically valid biochemical parameters of the thrombi in acute ischemic stroke

Abstract The significance and utility of innovative imaging techniques in arterial clot analysis, which enable far more detailed and automated analysis compared to standard methods, are presented. The examination of two types of human thrombi is shown, representing the main ischemic stroke etiologie...

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Autores principales:	Aneta Blat, Jakub Dybas, Karolina Chrabaszcz, Katarzyna Bulat, Agnieszka Jasztal, Magdalena Kaczmarska, Roman Pulyk, Tadeusz Popiela, Agnieszka Slowik, Kamilla Malek, Mateusz G. Adamski, Katarzyna M. Marzec
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d1506645241a416ea34cc675877cca5f
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spelling	oai:doaj.org-article:d1506645241a416ea34cc675877cca5f2021-12-02T15:09:39ZFTIR, Raman and AFM characterization of the clinically valid biochemical parameters of the thrombi in acute ischemic stroke10.1038/s41598-019-51932-02045-2322https://doaj.org/article/d1506645241a416ea34cc675877cca5f2019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-51932-0https://doaj.org/toc/2045-2322Abstract The significance and utility of innovative imaging techniques in arterial clot analysis, which enable far more detailed and automated analysis compared to standard methods, are presented. The examination of two types of human thrombi is shown, representing the main ischemic stroke etiologies: fibrin–predominant clot of large vessel origin and red blood cells–rich clot of cardioembolic origin. The synergy effect of Fourier–transform infrared spectroscopy (FTIR), Raman spectroscopy (RS) and atomic force microscopy (AFM) techniques supported by chemometrics in comparison with reference histological staining was presented. The main advantage of such approach refers to free–label and non–destructive quantitative imaging of clinically valid, biochemical parameters in whole sample (FTIR–low resolution) and selected regions (RS–ultra–high resolution). We may include here analysis of lipid content, its distribution and total degree of unsaturation as well as analysis of protein content (mainly fibrin and hemoproteins). The AFM studies enhanced the vibrational data, showed clearly shape and thickness of clot features as well as visualized the fibrin framework. The extraordinary sensitivity of FTIR and RS imaging toward detection and discrimination of clinically valid parameters in clot confirms its applicability in assessment of thrombi origin.Aneta BlatJakub DybasKarolina ChrabaszczKatarzyna BulatAgnieszka JasztalMagdalena KaczmarskaRoman PulykTadeusz PopielaAgnieszka SlowikKamilla MalekMateusz G. AdamskiKatarzyna M. MarzecNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aneta Blat Jakub Dybas Karolina Chrabaszcz Katarzyna Bulat Agnieszka Jasztal Magdalena Kaczmarska Roman Pulyk Tadeusz Popiela Agnieszka Slowik Kamilla Malek Mateusz G. Adamski Katarzyna M. Marzec FTIR, Raman and AFM characterization of the clinically valid biochemical parameters of the thrombi in acute ischemic stroke
description	Abstract The significance and utility of innovative imaging techniques in arterial clot analysis, which enable far more detailed and automated analysis compared to standard methods, are presented. The examination of two types of human thrombi is shown, representing the main ischemic stroke etiologies: fibrin–predominant clot of large vessel origin and red blood cells–rich clot of cardioembolic origin. The synergy effect of Fourier–transform infrared spectroscopy (FTIR), Raman spectroscopy (RS) and atomic force microscopy (AFM) techniques supported by chemometrics in comparison with reference histological staining was presented. The main advantage of such approach refers to free–label and non–destructive quantitative imaging of clinically valid, biochemical parameters in whole sample (FTIR–low resolution) and selected regions (RS–ultra–high resolution). We may include here analysis of lipid content, its distribution and total degree of unsaturation as well as analysis of protein content (mainly fibrin and hemoproteins). The AFM studies enhanced the vibrational data, showed clearly shape and thickness of clot features as well as visualized the fibrin framework. The extraordinary sensitivity of FTIR and RS imaging toward detection and discrimination of clinically valid parameters in clot confirms its applicability in assessment of thrombi origin.
format	article
author	Aneta Blat Jakub Dybas Karolina Chrabaszcz Katarzyna Bulat Agnieszka Jasztal Magdalena Kaczmarska Roman Pulyk Tadeusz Popiela Agnieszka Slowik Kamilla Malek Mateusz G. Adamski Katarzyna M. Marzec
author_facet	Aneta Blat Jakub Dybas Karolina Chrabaszcz Katarzyna Bulat Agnieszka Jasztal Magdalena Kaczmarska Roman Pulyk Tadeusz Popiela Agnieszka Slowik Kamilla Malek Mateusz G. Adamski Katarzyna M. Marzec
author_sort	Aneta Blat
title	FTIR, Raman and AFM characterization of the clinically valid biochemical parameters of the thrombi in acute ischemic stroke
title_short	FTIR, Raman and AFM characterization of the clinically valid biochemical parameters of the thrombi in acute ischemic stroke
title_full	FTIR, Raman and AFM characterization of the clinically valid biochemical parameters of the thrombi in acute ischemic stroke
title_fullStr	FTIR, Raman and AFM characterization of the clinically valid biochemical parameters of the thrombi in acute ischemic stroke
title_full_unstemmed	FTIR, Raman and AFM characterization of the clinically valid biochemical parameters of the thrombi in acute ischemic stroke
title_sort	ftir, raman and afm characterization of the clinically valid biochemical parameters of the thrombi in acute ischemic stroke
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/d1506645241a416ea34cc675877cca5f
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FTIR, Raman and AFM characterization of the clinically valid biochemical parameters of the thrombi in acute ischemic stroke

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