Characterization of G-Quadruplexes Folding/Unfolding Dynamics and Interactions with Proteins from Single-Molecule Force Spectroscopy

G-quadruplexes (G4s) are stable secondary nucleic acid structures that play crucial roles in many fundamental biological processes. The folding/unfolding dynamics of G4 structures are associated with the replication and transcription regulation functions of G4s. However, many DNA G4 sequences can ad...

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Autores principales:	Yuanlei Cheng, Yashuo Zhang, Huijuan You
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	single-molecule manipulations G-quadruplex polymorphism kinetics mechanical stability G4 helicase Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/d150c29c0ef549d3bfeaee23b081bf46
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id	oai:doaj.org-article:d150c29c0ef549d3bfeaee23b081bf46
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spelling	oai:doaj.org-article:d150c29c0ef549d3bfeaee23b081bf462021-11-25T16:52:13ZCharacterization of G-Quadruplexes Folding/Unfolding Dynamics and Interactions with Proteins from Single-Molecule Force Spectroscopy10.3390/biom111115792218-273Xhttps://doaj.org/article/d150c29c0ef549d3bfeaee23b081bf462021-10-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1579https://doaj.org/toc/2218-273XG-quadruplexes (G4s) are stable secondary nucleic acid structures that play crucial roles in many fundamental biological processes. The folding/unfolding dynamics of G4 structures are associated with the replication and transcription regulation functions of G4s. However, many DNA G4 sequences can adopt a variety of topologies and have complex folding/unfolding dynamics. Determining the dynamics of G4s and their regulation by proteins remains challenging due to the coexistence of multiple structures in a heterogeneous sample. Here, in this mini-review, we introduce the application of single-molecule force-spectroscopy methods, such as magnetic tweezers, optical tweezers, and atomic force microscopy, to characterize the polymorphism and folding/unfolding dynamics of G4s. We also briefly introduce recent studies using single-molecule force spectroscopy to study the molecular mechanisms of G4-interacting proteins.Yuanlei ChengYashuo ZhangHuijuan YouMDPI AGarticlesingle-molecule manipulationsG-quadruplexpolymorphismkineticsmechanical stabilityG4 helicaseMicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1579, p 1579 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	single-molecule manipulations G-quadruplex polymorphism kinetics mechanical stability G4 helicase Microbiology QR1-502
spellingShingle	single-molecule manipulations G-quadruplex polymorphism kinetics mechanical stability G4 helicase Microbiology QR1-502 Yuanlei Cheng Yashuo Zhang Huijuan You Characterization of G-Quadruplexes Folding/Unfolding Dynamics and Interactions with Proteins from Single-Molecule Force Spectroscopy
description	G-quadruplexes (G4s) are stable secondary nucleic acid structures that play crucial roles in many fundamental biological processes. The folding/unfolding dynamics of G4 structures are associated with the replication and transcription regulation functions of G4s. However, many DNA G4 sequences can adopt a variety of topologies and have complex folding/unfolding dynamics. Determining the dynamics of G4s and their regulation by proteins remains challenging due to the coexistence of multiple structures in a heterogeneous sample. Here, in this mini-review, we introduce the application of single-molecule force-spectroscopy methods, such as magnetic tweezers, optical tweezers, and atomic force microscopy, to characterize the polymorphism and folding/unfolding dynamics of G4s. We also briefly introduce recent studies using single-molecule force spectroscopy to study the molecular mechanisms of G4-interacting proteins.
format	article
author	Yuanlei Cheng Yashuo Zhang Huijuan You
author_facet	Yuanlei Cheng Yashuo Zhang Huijuan You
author_sort	Yuanlei Cheng
title	Characterization of G-Quadruplexes Folding/Unfolding Dynamics and Interactions with Proteins from Single-Molecule Force Spectroscopy
title_short	Characterization of G-Quadruplexes Folding/Unfolding Dynamics and Interactions with Proteins from Single-Molecule Force Spectroscopy
title_full	Characterization of G-Quadruplexes Folding/Unfolding Dynamics and Interactions with Proteins from Single-Molecule Force Spectroscopy
title_fullStr	Characterization of G-Quadruplexes Folding/Unfolding Dynamics and Interactions with Proteins from Single-Molecule Force Spectroscopy
title_full_unstemmed	Characterization of G-Quadruplexes Folding/Unfolding Dynamics and Interactions with Proteins from Single-Molecule Force Spectroscopy
title_sort	characterization of g-quadruplexes folding/unfolding dynamics and interactions with proteins from single-molecule force spectroscopy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d150c29c0ef549d3bfeaee23b081bf46
work_keys_str_mv	AT yuanleicheng characterizationofgquadruplexesfoldingunfoldingdynamicsandinteractionswithproteinsfromsinglemoleculeforcespectroscopy AT yashuozhang characterizationofgquadruplexesfoldingunfoldingdynamicsandinteractionswithproteinsfromsinglemoleculeforcespectroscopy AT huijuanyou characterizationofgquadruplexesfoldingunfoldingdynamicsandinteractionswithproteinsfromsinglemoleculeforcespectroscopy
_version_	1718412925862936576

Characterization of G-Quadruplexes Folding/Unfolding Dynamics and Interactions with Proteins from Single-Molecule Force Spectroscopy

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