Identification of a solo acylhomoserine lactone synthase from the myxobacterium Archangium gephyra

Abstract Considered a key taxon in soil and marine microbial communities, myxobacteria exist as coordinated swarms that utilize a combination of lytic enzymes and specialized metabolites to facilitate predation of microbes. This capacity to produce specialized metabolites and the associated abundanc...

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Autores principales:	Hanan Albataineh, Maya Duke, Sandeep K. Misra, Joshua S. Sharp, D. Cole Stevens
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d1514883bfe143359c0ed0f49fa7ec26
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spelling	oai:doaj.org-article:d1514883bfe143359c0ed0f49fa7ec262021-12-02T10:44:09ZIdentification of a solo acylhomoserine lactone synthase from the myxobacterium Archangium gephyra10.1038/s41598-021-82480-12045-2322https://doaj.org/article/d1514883bfe143359c0ed0f49fa7ec262021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82480-1https://doaj.org/toc/2045-2322Abstract Considered a key taxon in soil and marine microbial communities, myxobacteria exist as coordinated swarms that utilize a combination of lytic enzymes and specialized metabolites to facilitate predation of microbes. This capacity to produce specialized metabolites and the associated abundance of biosynthetic pathways contained within their genomes have motivated continued drug discovery efforts from myxobacteria. Of all myxobacterial biosynthetic gene clusters deposited in the antiSMASH database, only one putative acylhomoserine lactone (AHL) synthase, agpI, was observed, in genome data from Archangium gephyra. Without an AHL receptor also apparent in the genome of A. gephyra, we sought to determine if AgpI was an uncommon example of an orphaned AHL synthase. Herein we report the bioinformatic assessment of AgpI and discovery of a second AHL synthase from Vitiosangium sp. During axenic cultivation conditions, no detectible AHL metabolites were observed in A. gephyra extracts. However, heterologous expression of each synthase in Escherichia coli provided detectible quantities of 3 AHL signals including 2 known AHLs, C8-AHL and C9-AHL. These results suggest that A. gephyra AHL production is dormant during axenic cultivation. The functional, orphaned AHL synthase, AgpI, is unique to A. gephyra, and its utility to the predatory myxobacterium remains unknown.Hanan AlbatainehMaya DukeSandeep K. MisraJoshua S. SharpD. Cole StevensNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hanan Albataineh Maya Duke Sandeep K. Misra Joshua S. Sharp D. Cole Stevens Identification of a solo acylhomoserine lactone synthase from the myxobacterium Archangium gephyra
description	Abstract Considered a key taxon in soil and marine microbial communities, myxobacteria exist as coordinated swarms that utilize a combination of lytic enzymes and specialized metabolites to facilitate predation of microbes. This capacity to produce specialized metabolites and the associated abundance of biosynthetic pathways contained within their genomes have motivated continued drug discovery efforts from myxobacteria. Of all myxobacterial biosynthetic gene clusters deposited in the antiSMASH database, only one putative acylhomoserine lactone (AHL) synthase, agpI, was observed, in genome data from Archangium gephyra. Without an AHL receptor also apparent in the genome of A. gephyra, we sought to determine if AgpI was an uncommon example of an orphaned AHL synthase. Herein we report the bioinformatic assessment of AgpI and discovery of a second AHL synthase from Vitiosangium sp. During axenic cultivation conditions, no detectible AHL metabolites were observed in A. gephyra extracts. However, heterologous expression of each synthase in Escherichia coli provided detectible quantities of 3 AHL signals including 2 known AHLs, C8-AHL and C9-AHL. These results suggest that A. gephyra AHL production is dormant during axenic cultivation. The functional, orphaned AHL synthase, AgpI, is unique to A. gephyra, and its utility to the predatory myxobacterium remains unknown.
format	article
author	Hanan Albataineh Maya Duke Sandeep K. Misra Joshua S. Sharp D. Cole Stevens
author_facet	Hanan Albataineh Maya Duke Sandeep K. Misra Joshua S. Sharp D. Cole Stevens
author_sort	Hanan Albataineh
title	Identification of a solo acylhomoserine lactone synthase from the myxobacterium Archangium gephyra
title_short	Identification of a solo acylhomoserine lactone synthase from the myxobacterium Archangium gephyra
title_full	Identification of a solo acylhomoserine lactone synthase from the myxobacterium Archangium gephyra
title_fullStr	Identification of a solo acylhomoserine lactone synthase from the myxobacterium Archangium gephyra
title_full_unstemmed	Identification of a solo acylhomoserine lactone synthase from the myxobacterium Archangium gephyra
title_sort	identification of a solo acylhomoserine lactone synthase from the myxobacterium archangium gephyra
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d1514883bfe143359c0ed0f49fa7ec26
work_keys_str_mv	AT hananalbataineh identificationofasoloacylhomoserinelactonesynthasefromthemyxobacteriumarchangiumgephyra AT mayaduke identificationofasoloacylhomoserinelactonesynthasefromthemyxobacteriumarchangiumgephyra AT sandeepkmisra identificationofasoloacylhomoserinelactonesynthasefromthemyxobacteriumarchangiumgephyra AT joshuassharp identificationofasoloacylhomoserinelactonesynthasefromthemyxobacteriumarchangiumgephyra AT dcolestevens identificationofasoloacylhomoserinelactonesynthasefromthemyxobacteriumarchangiumgephyra
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Identification of a solo acylhomoserine lactone synthase from the myxobacterium Archangium gephyra

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