Hostile takeover by Plasmodium: reorganization of parasite and host cell membranes during liver stage egress.

The protozoan parasite Plasmodium is transmitted by female Anopheles mosquitoes and undergoes obligatory development within a parasitophorous vacuole in hepatocytes before it is released into the bloodstream. The transition to the blood stage was previously shown to involve the packaging of exoeryth...

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Autores principales:	Stefanie Graewe, Kathleen E Rankin, Christine Lehmann, Christina Deschermeier, Leonie Hecht, Ulrike Froehlke, Rebecca R Stanway, Volker Heussler
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d1555e3cf0184a8e818e7c6ccdc5996f
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spelling	oai:doaj.org-article:d1555e3cf0184a8e818e7c6ccdc5996f2021-11-18T06:03:01ZHostile takeover by Plasmodium: reorganization of parasite and host cell membranes during liver stage egress.1553-73661553-737410.1371/journal.ppat.1002224https://doaj.org/article/d1555e3cf0184a8e818e7c6ccdc5996f2011-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21909271/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374The protozoan parasite Plasmodium is transmitted by female Anopheles mosquitoes and undergoes obligatory development within a parasitophorous vacuole in hepatocytes before it is released into the bloodstream. The transition to the blood stage was previously shown to involve the packaging of exoerythrocytic merozoites into membrane-surrounded vesicles, called merosomes, which are delivered directly into liver sinusoids. However, it was unclear whether the membrane of these merosomes was derived from the parasite membrane, the parasitophorous vacuole membrane or the host cell membrane. This knowledge is required to determine how phagocytes will be directed against merosomes. Here, we fluorescently label the candidate membranes and use live cell imaging to show that the merosome membrane derives from the host cell membrane. We also demonstrate that proteins in the host cell membrane are lost during merozoite liberation from the parasitophorous vacuole. Immediately after the breakdown of the parasitophorous vacuole membrane, the host cell mitochondria begin to degenerate and protein biosynthesis arrests. The intact host cell plasma membrane surrounding merosomes allows Plasmodium to mask itself from the host immune system and bypass the numerous Kupffer cells on its way into the bloodstream. This represents an effective strategy for evading host defenses before establishing a blood stage infection.Stefanie GraeweKathleen E RankinChristine LehmannChristina DeschermeierLeonie HechtUlrike FroehlkeRebecca R StanwayVolker HeusslerPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 7, Iss 9, p e1002224 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Stefanie Graewe Kathleen E Rankin Christine Lehmann Christina Deschermeier Leonie Hecht Ulrike Froehlke Rebecca R Stanway Volker Heussler Hostile takeover by Plasmodium: reorganization of parasite and host cell membranes during liver stage egress.
description	The protozoan parasite Plasmodium is transmitted by female Anopheles mosquitoes and undergoes obligatory development within a parasitophorous vacuole in hepatocytes before it is released into the bloodstream. The transition to the blood stage was previously shown to involve the packaging of exoerythrocytic merozoites into membrane-surrounded vesicles, called merosomes, which are delivered directly into liver sinusoids. However, it was unclear whether the membrane of these merosomes was derived from the parasite membrane, the parasitophorous vacuole membrane or the host cell membrane. This knowledge is required to determine how phagocytes will be directed against merosomes. Here, we fluorescently label the candidate membranes and use live cell imaging to show that the merosome membrane derives from the host cell membrane. We also demonstrate that proteins in the host cell membrane are lost during merozoite liberation from the parasitophorous vacuole. Immediately after the breakdown of the parasitophorous vacuole membrane, the host cell mitochondria begin to degenerate and protein biosynthesis arrests. The intact host cell plasma membrane surrounding merosomes allows Plasmodium to mask itself from the host immune system and bypass the numerous Kupffer cells on its way into the bloodstream. This represents an effective strategy for evading host defenses before establishing a blood stage infection.
format	article
author	Stefanie Graewe Kathleen E Rankin Christine Lehmann Christina Deschermeier Leonie Hecht Ulrike Froehlke Rebecca R Stanway Volker Heussler
author_facet	Stefanie Graewe Kathleen E Rankin Christine Lehmann Christina Deschermeier Leonie Hecht Ulrike Froehlke Rebecca R Stanway Volker Heussler
author_sort	Stefanie Graewe
title	Hostile takeover by Plasmodium: reorganization of parasite and host cell membranes during liver stage egress.
title_short	Hostile takeover by Plasmodium: reorganization of parasite and host cell membranes during liver stage egress.
title_full	Hostile takeover by Plasmodium: reorganization of parasite and host cell membranes during liver stage egress.
title_fullStr	Hostile takeover by Plasmodium: reorganization of parasite and host cell membranes during liver stage egress.
title_full_unstemmed	Hostile takeover by Plasmodium: reorganization of parasite and host cell membranes during liver stage egress.
title_sort	hostile takeover by plasmodium: reorganization of parasite and host cell membranes during liver stage egress.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/d1555e3cf0184a8e818e7c6ccdc5996f
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Hostile takeover by Plasmodium: reorganization of parasite and host cell membranes during liver stage egress.

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