Optical waveguide lightmode spectroscopic techniques for investigating membrane-bound ion channel activities.

Optical waveguide lightmode spectroscopic (OWLS) techniques were probed for monitoring ion permeation through channels incorporated into artificial lipid environment. A novel sensor set-up was developed by depositing liposomes or cell-derived membrane fragments onto hydrophilic polytetrafluoroethyle...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Inna Székács, Nóra Kaszás, Pál Gróf, Katalin Erdélyi, István Szendrő, Balázs Mihalik, Agnes Pataki, Ferenc A Antoni, Emilia Madarász
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d156618b5c1d475aae8664981e8ddb5f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d156618b5c1d475aae8664981e8ddb5f
record_format	dspace
spelling	oai:doaj.org-article:d156618b5c1d475aae8664981e8ddb5f2021-11-18T08:42:45ZOptical waveguide lightmode spectroscopic techniques for investigating membrane-bound ion channel activities.1932-620310.1371/journal.pone.0081398https://doaj.org/article/d156618b5c1d475aae8664981e8ddb5f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24339925/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Optical waveguide lightmode spectroscopic (OWLS) techniques were probed for monitoring ion permeation through channels incorporated into artificial lipid environment. A novel sensor set-up was developed by depositing liposomes or cell-derived membrane fragments onto hydrophilic polytetrafluoroethylene (PTFE) membrane. The fibrous material of PTFE membrane could entrap lipoid vesicles and the water-filled pores provided environment for the hydrophilic domains of lipid-embedded proteins. The sensor surface was kept clean from the lipid holder PTFE membrane by a water- and ion-permeable polyethylene terephthalate (PET) mesh. The sensor set-up was tested with egg yolk lecithin liposomes containing gramicidin ion channels and with cell-derived membrane fragments enriched in GABA-gated anion channels. The method allowed monitoring the move of Na(+) and organic cations through gramicidin channels and detecting the Cl(-)-channel functions of the (α5β2γ2) GABAA receptor in the presence or absence of GABA and the competitive GABA-blocker bicuculline.Inna SzékácsNóra KaszásPál GrófKatalin ErdélyiIstván SzendrőBalázs MihalikAgnes PatakiFerenc A AntoniEmilia MadarászPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e81398 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Inna Székács Nóra Kaszás Pál Gróf Katalin Erdélyi István Szendrő Balázs Mihalik Agnes Pataki Ferenc A Antoni Emilia Madarász Optical waveguide lightmode spectroscopic techniques for investigating membrane-bound ion channel activities.
description	Optical waveguide lightmode spectroscopic (OWLS) techniques were probed for monitoring ion permeation through channels incorporated into artificial lipid environment. A novel sensor set-up was developed by depositing liposomes or cell-derived membrane fragments onto hydrophilic polytetrafluoroethylene (PTFE) membrane. The fibrous material of PTFE membrane could entrap lipoid vesicles and the water-filled pores provided environment for the hydrophilic domains of lipid-embedded proteins. The sensor surface was kept clean from the lipid holder PTFE membrane by a water- and ion-permeable polyethylene terephthalate (PET) mesh. The sensor set-up was tested with egg yolk lecithin liposomes containing gramicidin ion channels and with cell-derived membrane fragments enriched in GABA-gated anion channels. The method allowed monitoring the move of Na(+) and organic cations through gramicidin channels and detecting the Cl(-)-channel functions of the (α5β2γ2) GABAA receptor in the presence or absence of GABA and the competitive GABA-blocker bicuculline.
format	article
author	Inna Székács Nóra Kaszás Pál Gróf Katalin Erdélyi István Szendrő Balázs Mihalik Agnes Pataki Ferenc A Antoni Emilia Madarász
author_facet	Inna Székács Nóra Kaszás Pál Gróf Katalin Erdélyi István Szendrő Balázs Mihalik Agnes Pataki Ferenc A Antoni Emilia Madarász
author_sort	Inna Székács
title	Optical waveguide lightmode spectroscopic techniques for investigating membrane-bound ion channel activities.
title_short	Optical waveguide lightmode spectroscopic techniques for investigating membrane-bound ion channel activities.
title_full	Optical waveguide lightmode spectroscopic techniques for investigating membrane-bound ion channel activities.
title_fullStr	Optical waveguide lightmode spectroscopic techniques for investigating membrane-bound ion channel activities.
title_full_unstemmed	Optical waveguide lightmode spectroscopic techniques for investigating membrane-bound ion channel activities.
title_sort	optical waveguide lightmode spectroscopic techniques for investigating membrane-bound ion channel activities.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/d156618b5c1d475aae8664981e8ddb5f
work_keys_str_mv	AT innaszekacs opticalwaveguidelightmodespectroscopictechniquesforinvestigatingmembraneboundionchannelactivities AT norakaszas opticalwaveguidelightmodespectroscopictechniquesforinvestigatingmembraneboundionchannelactivities AT palgrof opticalwaveguidelightmodespectroscopictechniquesforinvestigatingmembraneboundionchannelactivities AT katalinerdelyi opticalwaveguidelightmodespectroscopictechniquesforinvestigatingmembraneboundionchannelactivities AT istvanszendro opticalwaveguidelightmodespectroscopictechniquesforinvestigatingmembraneboundionchannelactivities AT balazsmihalik opticalwaveguidelightmodespectroscopictechniquesforinvestigatingmembraneboundionchannelactivities AT agnespataki opticalwaveguidelightmodespectroscopictechniquesforinvestigatingmembraneboundionchannelactivities AT ferencaantoni opticalwaveguidelightmodespectroscopictechniquesforinvestigatingmembraneboundionchannelactivities AT emiliamadarasz opticalwaveguidelightmodespectroscopictechniquesforinvestigatingmembraneboundionchannelactivities
_version_	1718421411397107712

Optical waveguide lightmode spectroscopic techniques for investigating membrane-bound ion channel activities.

Ejemplares similares