Study of relationships between HLA-G gene polymorphism, intrauterine infection and recurrent miscarriage in women

The relationship between the HLA-G gene polymorphism (rs41551813, rs12722477, rs41557518), intrauterine infection and recurrent miscarriage (RM) in women were studied. The case group consisted of 180 patients with RM, defined as two or more consecutive miscarriages (min = 2; max = 8) at up to 20 wee...

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Autores principales:	L. A. Gordeeva, E. N. Voronina, E. G. Polenok, S. A. Mun, S. L. Nersesyan, R. V. Olennikova, M. L. Filipenko, A. N. Glushkov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2021
Materias:	genetic polymorphism hla-g recurrent miscarriage Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/d157c6db0ee640d88f313774078e903a
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spelling	oai:doaj.org-article:d157c6db0ee640d88f313774078e903a2021-11-18T08:03:50ZStudy of relationships between HLA-G gene polymorphism, intrauterine infection and recurrent miscarriage in women1563-06252313-741X10.15789/1563-0625-SOR-2155https://doaj.org/article/d157c6db0ee640d88f313774078e903a2021-05-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/2155https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe relationship between the HLA-G gene polymorphism (rs41551813, rs12722477, rs41557518), intrauterine infection and recurrent miscarriage (RM) in women were studied. The case group consisted of 180 patients with RM, defined as two or more consecutive miscarriages (min = 2; max = 8) at up to 20 weeks of gestation, and with clinically confirmed pregnancies and non-viable fetuses. At the time of examination. the women were enrolled from the Genetic Counseling Center at the Kemerovo Regional Clinical Hospital, Kemerovo, Russia, and were not pregnant. Each patient underwent a gynecological examination. We excluded women with a history of medical abortion, birth, and ectopic pregnancies. In addition, we excluded women with endocrine (e.g. diabetes) disorders. To exclude other known causes of spontaneous abortion, the following tests were performed: ultrasound examination of pelvic organs, and karyotyping in women and men. The women’s mean age in the RM group, was 29.6±4.8 (SD) years. The control group comprised 408 fertile women. These women didn’t have a history of spontaneous abortion, or a family history of congenital malformations. They have born, at least, 1-2 healthy children. Women’s mean age at birth of last child was 26.8±5.2 (SD) years. Influence of the intrauterine infection was analyzed on the basis of laboratory tests. Diagnostics of bacterial vaginosis and vulvo-vaginal candidiasis by microscopic examination was conducted. Viral agent infections (herpes simplex virus type 2, cytomegalovirus, human papilloma virus type 16/18), Chlamydia trachomatis, Mycoplasma hominis, Ureaplasma urealyticum, Gardnerella vaginalis and Trichomonas vaginalis were detected by enzyme-linked immunoassay and polymerase chain reaction (PCR). The data were obtained from the medical cards of the surveyed women. All the women gave a written informed consent before participating in the study. Typing of polymorphisms of Thr31Ser (rs41551813, HLA-G01:03) in exon 2, Leu110Ile (rs12722477, HLA-G01:04) and 1597 delС (rs41557518, HLA-G01:05N) in exon 3 HLA-G genes were performed by realtime PCR followed by melting analysis. The study showed that the intrauterine infection was not a risk factor for RM (p = 0.30) in the examined women. It was found that the 110 Ile allele (HLA-G 01:04) was a risk factor for RM both in women with intrauterine infection [ORa = 4.50 (2.41-8.38), p = 2.09e-06], and in women without infection [ORa = 2.46 (1.44-4.21), p = 0.0009]. The cooperative influence of genetic and infections factors with the risk of RM in women was revealed [ORa+f = 3.50 (2.01-6.09), p = 8.78e-06]. Our results will be useful in understanding the molecular mechanisms of immune disorders in fetomaternal interface, and for choosing the strategy of management and treatment in women with RM.L. A. GordeevaE. N. VoroninaE. G. PolenokS. A. MunS. L. NersesyanR. V. OlennikovaM. L. FilipenkoA. N. GlushkovSPb RAACIarticlegenetic polymorphismhla-grecurrent miscarriageImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 23, Iss 2, Pp 369-380 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	genetic polymorphism hla-g recurrent miscarriage Immunologic diseases. Allergy RC581-607
spellingShingle	genetic polymorphism hla-g recurrent miscarriage Immunologic diseases. Allergy RC581-607 L. A. Gordeeva E. N. Voronina E. G. Polenok S. A. Mun S. L. Nersesyan R. V. Olennikova M. L. Filipenko A. N. Glushkov Study of relationships between HLA-G gene polymorphism, intrauterine infection and recurrent miscarriage in women
description	The relationship between the HLA-G gene polymorphism (rs41551813, rs12722477, rs41557518), intrauterine infection and recurrent miscarriage (RM) in women were studied. The case group consisted of 180 patients with RM, defined as two or more consecutive miscarriages (min = 2; max = 8) at up to 20 weeks of gestation, and with clinically confirmed pregnancies and non-viable fetuses. At the time of examination. the women were enrolled from the Genetic Counseling Center at the Kemerovo Regional Clinical Hospital, Kemerovo, Russia, and were not pregnant. Each patient underwent a gynecological examination. We excluded women with a history of medical abortion, birth, and ectopic pregnancies. In addition, we excluded women with endocrine (e.g. diabetes) disorders. To exclude other known causes of spontaneous abortion, the following tests were performed: ultrasound examination of pelvic organs, and karyotyping in women and men. The women’s mean age in the RM group, was 29.6±4.8 (SD) years. The control group comprised 408 fertile women. These women didn’t have a history of spontaneous abortion, or a family history of congenital malformations. They have born, at least, 1-2 healthy children. Women’s mean age at birth of last child was 26.8±5.2 (SD) years. Influence of the intrauterine infection was analyzed on the basis of laboratory tests. Diagnostics of bacterial vaginosis and vulvo-vaginal candidiasis by microscopic examination was conducted. Viral agent infections (herpes simplex virus type 2, cytomegalovirus, human papilloma virus type 16/18), Chlamydia trachomatis, Mycoplasma hominis, Ureaplasma urealyticum, Gardnerella vaginalis and Trichomonas vaginalis were detected by enzyme-linked immunoassay and polymerase chain reaction (PCR). The data were obtained from the medical cards of the surveyed women. All the women gave a written informed consent before participating in the study. Typing of polymorphisms of Thr31Ser (rs41551813, HLA-G01:03) in exon 2, Leu110Ile (rs12722477, HLA-G01:04) and 1597 delС (rs41557518, HLA-G01:05N) in exon 3 HLA-G genes were performed by realtime PCR followed by melting analysis. The study showed that the intrauterine infection was not a risk factor for RM (p = 0.30) in the examined women. It was found that the 110 Ile allele (HLA-G 01:04) was a risk factor for RM both in women with intrauterine infection [ORa = 4.50 (2.41-8.38), p = 2.09e-06], and in women without infection [ORa = 2.46 (1.44-4.21), p = 0.0009]. The cooperative influence of genetic and infections factors with the risk of RM in women was revealed [ORa+f = 3.50 (2.01-6.09), p = 8.78e-06]. Our results will be useful in understanding the molecular mechanisms of immune disorders in fetomaternal interface, and for choosing the strategy of management and treatment in women with RM.
format	article
author	L. A. Gordeeva E. N. Voronina E. G. Polenok S. A. Mun S. L. Nersesyan R. V. Olennikova M. L. Filipenko A. N. Glushkov
author_facet	L. A. Gordeeva E. N. Voronina E. G. Polenok S. A. Mun S. L. Nersesyan R. V. Olennikova M. L. Filipenko A. N. Glushkov
author_sort	L. A. Gordeeva
title	Study of relationships between HLA-G gene polymorphism, intrauterine infection and recurrent miscarriage in women
title_short	Study of relationships between HLA-G gene polymorphism, intrauterine infection and recurrent miscarriage in women
title_full	Study of relationships between HLA-G gene polymorphism, intrauterine infection and recurrent miscarriage in women
title_fullStr	Study of relationships between HLA-G gene polymorphism, intrauterine infection and recurrent miscarriage in women
title_full_unstemmed	Study of relationships between HLA-G gene polymorphism, intrauterine infection and recurrent miscarriage in women
title_sort	study of relationships between hla-g gene polymorphism, intrauterine infection and recurrent miscarriage in women
publisher	SPb RAACI
publishDate	2021
url	https://doaj.org/article/d157c6db0ee640d88f313774078e903a
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Study of relationships between HLA-G gene polymorphism, intrauterine infection and recurrent miscarriage in women

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