miR-301a-5p Regulates <i>TGFB2</i> during Chicken Spermatogenesis

The process of spermatogenesis is complex and systemic, requiring the cooperation of many regulators. However, little is known about how micro RNAs (miRNAs) regulate spermatogenesis in poultry. In this study, we investigated key miRNAs and their target genes that are involved in spermatogenesis in c...

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Autores principales:	Qixin Guo, Yong Jiang, Hao Bai, Guohong Chen, Guobin Chang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	miR-301a-5p TGFB signaling pathway spermatogenesis chicken Genetics QH426-470
Acceso en línea:	https://doaj.org/article/d159d66ba813476990e7c35bdb588858
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spelling	oai:doaj.org-article:d159d66ba813476990e7c35bdb5888582021-11-25T17:40:54ZmiR-301a-5p Regulates <i>TGFB2</i> during Chicken Spermatogenesis10.3390/genes121116952073-4425https://doaj.org/article/d159d66ba813476990e7c35bdb5888582021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1695https://doaj.org/toc/2073-4425The process of spermatogenesis is complex and systemic, requiring the cooperation of many regulators. However, little is known about how micro RNAs (miRNAs) regulate spermatogenesis in poultry. In this study, we investigated key miRNAs and their target genes that are involved in spermatogenesis in chickens. Next-generation sequencing was conducted to determine miRNA expression profiles in five cell types: primordial germ cells (PGCs), spermatogonial stem cells (SSCs), spermatogonia (Spa), and chicken sperm. Next, we analyzed and identified several key miRNAs that regulate spermatogenesis in the four germline cell miRNA profiles. Among the enriched miRNAs, miRNA-301a-5p was the key miRNA in PGCs, SSCs, and Spa. Through reverse transcription quantitative PCR (RT-qPCR), dual-luciferase, and miRNA salience, we confirmed that miR-301a-5p binds to transforming growth factor-beta 2 (<i>TGFβ2</i>) and is involved in the transforming growth factor-beta (TGF-β) signaling pathway and germ cell development. To the best of our knowledge, this is the first demonstration of miR-301a-5p involvement in spermatogenesis by direct binding to <i>TGFβ2</i>, a key gene in the TGF-β signaling pathway. This finding contributes to the insights into the molecular mechanism through which miRNAs regulate germline cell differentiation and spermatogenesis in chickens.Qixin GuoYong JiangHao BaiGuohong ChenGuobin ChangMDPI AGarticlemiR-301a-5pTGFB signaling pathwayspermatogenesischickenGeneticsQH426-470ENGenes, Vol 12, Iss 1695, p 1695 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	miR-301a-5p TGFB signaling pathway spermatogenesis chicken Genetics QH426-470
spellingShingle	miR-301a-5p TGFB signaling pathway spermatogenesis chicken Genetics QH426-470 Qixin Guo Yong Jiang Hao Bai Guohong Chen Guobin Chang miR-301a-5p Regulates <i>TGFB2</i> during Chicken Spermatogenesis
description	The process of spermatogenesis is complex and systemic, requiring the cooperation of many regulators. However, little is known about how micro RNAs (miRNAs) regulate spermatogenesis in poultry. In this study, we investigated key miRNAs and their target genes that are involved in spermatogenesis in chickens. Next-generation sequencing was conducted to determine miRNA expression profiles in five cell types: primordial germ cells (PGCs), spermatogonial stem cells (SSCs), spermatogonia (Spa), and chicken sperm. Next, we analyzed and identified several key miRNAs that regulate spermatogenesis in the four germline cell miRNA profiles. Among the enriched miRNAs, miRNA-301a-5p was the key miRNA in PGCs, SSCs, and Spa. Through reverse transcription quantitative PCR (RT-qPCR), dual-luciferase, and miRNA salience, we confirmed that miR-301a-5p binds to transforming growth factor-beta 2 (<i>TGFβ2</i>) and is involved in the transforming growth factor-beta (TGF-β) signaling pathway and germ cell development. To the best of our knowledge, this is the first demonstration of miR-301a-5p involvement in spermatogenesis by direct binding to <i>TGFβ2</i>, a key gene in the TGF-β signaling pathway. This finding contributes to the insights into the molecular mechanism through which miRNAs regulate germline cell differentiation and spermatogenesis in chickens.
format	article
author	Qixin Guo Yong Jiang Hao Bai Guohong Chen Guobin Chang
author_facet	Qixin Guo Yong Jiang Hao Bai Guohong Chen Guobin Chang
author_sort	Qixin Guo
title	miR-301a-5p Regulates <i>TGFB2</i> during Chicken Spermatogenesis
title_short	miR-301a-5p Regulates <i>TGFB2</i> during Chicken Spermatogenesis
title_full	miR-301a-5p Regulates <i>TGFB2</i> during Chicken Spermatogenesis
title_fullStr	miR-301a-5p Regulates <i>TGFB2</i> during Chicken Spermatogenesis
title_full_unstemmed	miR-301a-5p Regulates <i>TGFB2</i> during Chicken Spermatogenesis
title_sort	mir-301a-5p regulates <i>tgfb2</i> during chicken spermatogenesis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d159d66ba813476990e7c35bdb588858
work_keys_str_mv	AT qixinguo mir301a5pregulatesitgfb2iduringchickenspermatogenesis AT yongjiang mir301a5pregulatesitgfb2iduringchickenspermatogenesis AT haobai mir301a5pregulatesitgfb2iduringchickenspermatogenesis AT guohongchen mir301a5pregulatesitgfb2iduringchickenspermatogenesis AT guobinchang mir301a5pregulatesitgfb2iduringchickenspermatogenesis
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miR-301a-5p Regulates <i>TGFB2</i> during Chicken Spermatogenesis

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