Characterization of complexation of poly (N-isopropylacrylamide-co-2-(dimethylamino) ethyl methacrylate) thermoresponsive cationic nanogels with salmon sperm DNA

Jim Moselhy1, Tasnim Vira1, Fei-Fei Liu2, Xiao Yu Wu11Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, Canada; 2Ontario Cancer Institute, Toronto, ON, CanadaAbstract: Thermoresponsive cationic nanogel (TCNG) networks based on N-isopropylacryl...

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Autores principales:	Jim Moselhy, Tasnim Vira, Fei-Fei Liu, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2009
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d15a471c68f1428f836e76cce36133ae
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spelling	oai:doaj.org-article:d15a471c68f1428f836e76cce36133ae2021-12-02T00:43:21ZCharacterization of complexation of poly (N-isopropylacrylamide-co-2-(dimethylamino) ethyl methacrylate) thermoresponsive cationic nanogels with salmon sperm DNA1176-91141178-2013https://doaj.org/article/d15a471c68f1428f836e76cce36133ae2009-08-01T00:00:00Zhttp://www.dovepress.com/characterization-of-complexation-of-poly-n-isopropylacrylamide-co-2-di-a3479https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Jim Moselhy1, Tasnim Vira1, Fei-Fei Liu2, Xiao Yu Wu11Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, Canada; 2Ontario Cancer Institute, Toronto, ON, CanadaAbstract: Thermoresponsive cationic nanogel (TCNG) networks based on N-isopropylacrylamide (NIPAM), 2-(dimethylamino)ethyl methacrylate (DMAEMA), and quaternary alkyl ammonium halide salts of DMAEMA (DMAEMAQ) were synthesized by dispersion polymerization technique. The thermoresponsive properties of TCNGs and TCNG-salmon sperm DNA (sasDNA) polyplexes were characterized in aqueous media of various pH and ionic strength. P[NIPAM] and P[NIPAM/DMAEMA] TCNGs exhibited sharp volume phase transition (VPT) in water at critical temperatures (Tc) of 32 &deg;C and 36 &deg;C, respectively. Quaternized P[NIPAM/DMAEMAQ] TCNGs did not undergo sharp VPT up to 50 &deg;C. The VPT of uncomplexed TCNGs were sensitive to the ionic composition and ionic strength of salts in solution, but were insensitive to pH in the range 5.0 to 7.4. The VPT of P[NIPAM/DMAEMAQ]/sasDNA diminished in magnitude with increasing Wp/Wd suggesting greater compaction of the polyplexes. The distinct phase-transition properties of P[NIPAM/DMAEMA]/sasDNA and P[NIPAM/DMAEMAQ]/sasDNA polyplexes were attributed to the condensing capability of polycations and to differences in the spatial distribution of structural charges in quaternized and nonquaternized networks. The findings demonstrate that stable TCNGs can be prepared with controllable responsive properties determined by the nature of the cationic charge incorporated and may have potential as vehicles for DNA delivery.Keywords: poly(N-isopropyl acryamide), poly(2-dimethylamino)ethyl methacrylate, polyplex, thermoresponsive cationic nanogels Jim MoselhyTasnim ViraFei-Fei Liuet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2009, Iss default, Pp 153-164 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Jim Moselhy Tasnim Vira Fei-Fei Liu et al Characterization of complexation of poly (N-isopropylacrylamide-co-2-(dimethylamino) ethyl methacrylate) thermoresponsive cationic nanogels with salmon sperm DNA
description	Jim Moselhy1, Tasnim Vira1, Fei-Fei Liu2, Xiao Yu Wu11Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, Canada; 2Ontario Cancer Institute, Toronto, ON, CanadaAbstract: Thermoresponsive cationic nanogel (TCNG) networks based on N-isopropylacrylamide (NIPAM), 2-(dimethylamino)ethyl methacrylate (DMAEMA), and quaternary alkyl ammonium halide salts of DMAEMA (DMAEMAQ) were synthesized by dispersion polymerization technique. The thermoresponsive properties of TCNGs and TCNG-salmon sperm DNA (sasDNA) polyplexes were characterized in aqueous media of various pH and ionic strength. P[NIPAM] and P[NIPAM/DMAEMA] TCNGs exhibited sharp volume phase transition (VPT) in water at critical temperatures (Tc) of 32 &deg;C and 36 &deg;C, respectively. Quaternized P[NIPAM/DMAEMAQ] TCNGs did not undergo sharp VPT up to 50 &deg;C. The VPT of uncomplexed TCNGs were sensitive to the ionic composition and ionic strength of salts in solution, but were insensitive to pH in the range 5.0 to 7.4. The VPT of P[NIPAM/DMAEMAQ]/sasDNA diminished in magnitude with increasing Wp/Wd suggesting greater compaction of the polyplexes. The distinct phase-transition properties of P[NIPAM/DMAEMA]/sasDNA and P[NIPAM/DMAEMAQ]/sasDNA polyplexes were attributed to the condensing capability of polycations and to differences in the spatial distribution of structural charges in quaternized and nonquaternized networks. The findings demonstrate that stable TCNGs can be prepared with controllable responsive properties determined by the nature of the cationic charge incorporated and may have potential as vehicles for DNA delivery.Keywords: poly(N-isopropyl acryamide), poly(2-dimethylamino)ethyl methacrylate, polyplex, thermoresponsive cationic nanogels
format	article
author	Jim Moselhy Tasnim Vira Fei-Fei Liu et al
author_facet	Jim Moselhy Tasnim Vira Fei-Fei Liu et al
author_sort	Jim Moselhy
title	Characterization of complexation of poly (N-isopropylacrylamide-co-2-(dimethylamino) ethyl methacrylate) thermoresponsive cationic nanogels with salmon sperm DNA
title_short	Characterization of complexation of poly (N-isopropylacrylamide-co-2-(dimethylamino) ethyl methacrylate) thermoresponsive cationic nanogels with salmon sperm DNA
title_full	Characterization of complexation of poly (N-isopropylacrylamide-co-2-(dimethylamino) ethyl methacrylate) thermoresponsive cationic nanogels with salmon sperm DNA
title_fullStr	Characterization of complexation of poly (N-isopropylacrylamide-co-2-(dimethylamino) ethyl methacrylate) thermoresponsive cationic nanogels with salmon sperm DNA
title_full_unstemmed	Characterization of complexation of poly (N-isopropylacrylamide-co-2-(dimethylamino) ethyl methacrylate) thermoresponsive cationic nanogels with salmon sperm DNA
title_sort	characterization of complexation of poly (n-isopropylacrylamide-co-2-(dimethylamino) ethyl methacrylate) thermoresponsive cationic nanogels with salmon sperm dna
publisher	Dove Medical Press
publishDate	2009
url	https://doaj.org/article/d15a471c68f1428f836e76cce36133ae
work_keys_str_mv	AT jimmoselhy characterizationofcomplexationofpolynisopropylacrylamideco2dimethylaminoethylmethacrylatethermoresponsivecationicnanogelswithsalmonspermdna AT tasnimvira characterizationofcomplexationofpolynisopropylacrylamideco2dimethylaminoethylmethacrylatethermoresponsivecationicnanogelswithsalmonspermdna AT feifeiliu characterizationofcomplexationofpolynisopropylacrylamideco2dimethylaminoethylmethacrylatethermoresponsivecationicnanogelswithsalmonspermdna AT etal characterizationofcomplexationofpolynisopropylacrylamideco2dimethylaminoethylmethacrylatethermoresponsivecationicnanogelswithsalmonspermdna
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Characterization of complexation of poly (N-isopropylacrylamide-co-2-(dimethylamino) ethyl methacrylate) thermoresponsive cationic nanogels with salmon sperm DNA

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