Effects of ammonia on hypothalamic-pituitary-ovarian axis in female rabbits

Background: As one of the most harmful gases in the livestock house, ammonia is recognized as an environmental stressor by Environmental Protection Agency (United States). The study aimed to explore the effect of ammonia on hypothalamic-pituitary-ovarian (HPO) axis of rabbits. A total of ninety two-...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jia Cui, Fengyang Wu, Xinyu Yang, Shudong Liu, Shuaijuan Han, Baojiang Chen
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Ammonia HPO axis Rabbit Hormone secretion disorder Apoptosis Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/d15f911d82ea456eb8963bf66d1f33e4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d15f911d82ea456eb8963bf66d1f33e4
record_format	dspace
spelling	oai:doaj.org-article:d15f911d82ea456eb8963bf66d1f33e42021-11-06T04:17:30ZEffects of ammonia on hypothalamic-pituitary-ovarian axis in female rabbits0147-651310.1016/j.ecoenv.2021.112922https://doaj.org/article/d15f911d82ea456eb8963bf66d1f33e42021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321010344https://doaj.org/toc/0147-6513Background: As one of the most harmful gases in the livestock house, ammonia is recognized as an environmental stressor by Environmental Protection Agency (United States). The study aimed to explore the effect of ammonia on hypothalamic-pituitary-ovarian (HPO) axis of rabbits. A total of ninety two-month-old female IRA rabbits were randomly divided into three groups, and were kept in animal environment control rooms for four weeks at college of animal science and technology, Hebei Agricultural University (Baoding, China). The rabbits in the control group were kept under ammonia concentration of < 3 ppm. The two treatment groups were kept under ammonia concentration of 30 ppm and 50 ppm. Hypothalamus, pituitary, and ovary were collected for hematoxylin and eosin (HE) staining, immunohistochemistry, terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) and quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Serum was collected for enzyme-linked immunosorbent assay (ELISA). Results: Histopathological examination revealed that exposed to excess ammonia damaged the morphology and structure of hypothalamus, pituitary, and ovary. TUNEL assay revealed that apoptosis rate increased in hypothalamus, pituitary, and ovary. The protein expression levels of Bcl-2associated X protein (Bax) and Caspase-9 increased, while B-cell lymphoma-2 (Bcl-2) decreased, resulting in apoptosis. Moreover, the concentration of gonadotropin-releasing hormone (GnRH), follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), and progesterone (PROG) reduced in plasma. The mRNA expression of FSH and LH in pituitary and follicle-stimulating hormone receptor (FSHR), E2, PROG in ovary as well as decreased, indicated hormone secretion disorder. Conclusions: The results indicated that ammonia exposure damaged hypothalamus, pituitary, and ovary, caused hormone secretion disorder and apoptosis.Jia CuiFengyang WuXinyu YangShudong LiuShuaijuan HanBaojiang ChenElsevierarticleAmmoniaHPO axisRabbitHormone secretion disorderApoptosisEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 227, Iss , Pp 112922- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ammonia HPO axis Rabbit Hormone secretion disorder Apoptosis Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	Ammonia HPO axis Rabbit Hormone secretion disorder Apoptosis Environmental pollution TD172-193.5 Environmental sciences GE1-350 Jia Cui Fengyang Wu Xinyu Yang Shudong Liu Shuaijuan Han Baojiang Chen Effects of ammonia on hypothalamic-pituitary-ovarian axis in female rabbits
description	Background: As one of the most harmful gases in the livestock house, ammonia is recognized as an environmental stressor by Environmental Protection Agency (United States). The study aimed to explore the effect of ammonia on hypothalamic-pituitary-ovarian (HPO) axis of rabbits. A total of ninety two-month-old female IRA rabbits were randomly divided into three groups, and were kept in animal environment control rooms for four weeks at college of animal science and technology, Hebei Agricultural University (Baoding, China). The rabbits in the control group were kept under ammonia concentration of < 3 ppm. The two treatment groups were kept under ammonia concentration of 30 ppm and 50 ppm. Hypothalamus, pituitary, and ovary were collected for hematoxylin and eosin (HE) staining, immunohistochemistry, terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) and quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Serum was collected for enzyme-linked immunosorbent assay (ELISA). Results: Histopathological examination revealed that exposed to excess ammonia damaged the morphology and structure of hypothalamus, pituitary, and ovary. TUNEL assay revealed that apoptosis rate increased in hypothalamus, pituitary, and ovary. The protein expression levels of Bcl-2associated X protein (Bax) and Caspase-9 increased, while B-cell lymphoma-2 (Bcl-2) decreased, resulting in apoptosis. Moreover, the concentration of gonadotropin-releasing hormone (GnRH), follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), and progesterone (PROG) reduced in plasma. The mRNA expression of FSH and LH in pituitary and follicle-stimulating hormone receptor (FSHR), E2, PROG in ovary as well as decreased, indicated hormone secretion disorder. Conclusions: The results indicated that ammonia exposure damaged hypothalamus, pituitary, and ovary, caused hormone secretion disorder and apoptosis.
format	article
author	Jia Cui Fengyang Wu Xinyu Yang Shudong Liu Shuaijuan Han Baojiang Chen
author_facet	Jia Cui Fengyang Wu Xinyu Yang Shudong Liu Shuaijuan Han Baojiang Chen
author_sort	Jia Cui
title	Effects of ammonia on hypothalamic-pituitary-ovarian axis in female rabbits
title_short	Effects of ammonia on hypothalamic-pituitary-ovarian axis in female rabbits
title_full	Effects of ammonia on hypothalamic-pituitary-ovarian axis in female rabbits
title_fullStr	Effects of ammonia on hypothalamic-pituitary-ovarian axis in female rabbits
title_full_unstemmed	Effects of ammonia on hypothalamic-pituitary-ovarian axis in female rabbits
title_sort	effects of ammonia on hypothalamic-pituitary-ovarian axis in female rabbits
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/d15f911d82ea456eb8963bf66d1f33e4
work_keys_str_mv	AT jiacui effectsofammoniaonhypothalamicpituitaryovarianaxisinfemalerabbits AT fengyangwu effectsofammoniaonhypothalamicpituitaryovarianaxisinfemalerabbits AT xinyuyang effectsofammoniaonhypothalamicpituitaryovarianaxisinfemalerabbits AT shudongliu effectsofammoniaonhypothalamicpituitaryovarianaxisinfemalerabbits AT shuaijuanhan effectsofammoniaonhypothalamicpituitaryovarianaxisinfemalerabbits AT baojiangchen effectsofammoniaonhypothalamicpituitaryovarianaxisinfemalerabbits
_version_	1718443945976922112

Effects of ammonia on hypothalamic-pituitary-ovarian axis in female rabbits

Ejemplares similares