Mir-15A Reconstitution in Prostate Cancer Cell Line Suppresses Cancer Progression Through Down Regulation of MYB and Androgen Receptor Upregulation

Prostate cancer is one of the most common malignancies and the second leading cause of death from cancer in men. MicroRNAs are noncoding RNAs that have a role of post-transcriptional regulators. In this study we investigated how the tumour suppressor miR-15a modulates main transcription factors like...

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Autores principales:	Todorova K., Hayrabedyan S.
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2015
Materias:	prostate cancer microrna ar cmyb Medicine R
Acceso en línea:	https://doaj.org/article/d1644c10e2b943ee882759a2462eacab
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spelling	oai:doaj.org-article:d1644c10e2b943ee882759a2462eacab2021-12-02T17:31:48ZMir-15A Reconstitution in Prostate Cancer Cell Line Suppresses Cancer Progression Through Down Regulation of MYB and Androgen Receptor Upregulation0324-175010.1515/amb-2015-0003https://doaj.org/article/d1644c10e2b943ee882759a2462eacab2015-05-01T00:00:00Zhttps://doi.org/10.1515/amb-2015-0003https://doaj.org/toc/0324-1750Prostate cancer is one of the most common malignancies and the second leading cause of death from cancer in men. MicroRNAs are noncoding RNAs that have a role of post-transcriptional regulators. In this study we investigated how the tumour suppressor miR-15a modulates main transcription factors like cMYB and AR in androgen sensitive prostate cancer cell line LNCaP. The miR-15a inhibitor, mimic, and their negative controls were transfected into LNCaP cells. Real-time PCR analysis was performed in order to estimate the transcript levels of cMYB and AR. Flow cytometry analysis was performed to measure the protein levels of cMYB and AR. A Cell migration assay was done for cells transfected with miR-15a inhibitor and mimic. We found that cMYB is down-regulated and AR is up-regulated by miR-15a on the transcriptional and protein levels. By reconstituting miR-15a, we found that its down regulation in prostate cancer contributes to cMYB-induced cancer progression and reduced androgen receptivity. The ability of miR-15a to suppress cancer cell viability and migration is a very important phenomenon for understanding cancer heterogeneity in regard to adapted therapeutic approach development.Todorova K.Hayrabedyan S.Sciendoarticleprostate cancermicrornaarcmybMedicineRENActa Medica Bulgarica, Vol 42, Iss 1, Pp 18-22 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	prostate cancer microrna ar cmyb Medicine R
spellingShingle	prostate cancer microrna ar cmyb Medicine R Todorova K. Hayrabedyan S. Mir-15A Reconstitution in Prostate Cancer Cell Line Suppresses Cancer Progression Through Down Regulation of MYB and Androgen Receptor Upregulation
description	Prostate cancer is one of the most common malignancies and the second leading cause of death from cancer in men. MicroRNAs are noncoding RNAs that have a role of post-transcriptional regulators. In this study we investigated how the tumour suppressor miR-15a modulates main transcription factors like cMYB and AR in androgen sensitive prostate cancer cell line LNCaP. The miR-15a inhibitor, mimic, and their negative controls were transfected into LNCaP cells. Real-time PCR analysis was performed in order to estimate the transcript levels of cMYB and AR. Flow cytometry analysis was performed to measure the protein levels of cMYB and AR. A Cell migration assay was done for cells transfected with miR-15a inhibitor and mimic. We found that cMYB is down-regulated and AR is up-regulated by miR-15a on the transcriptional and protein levels. By reconstituting miR-15a, we found that its down regulation in prostate cancer contributes to cMYB-induced cancer progression and reduced androgen receptivity. The ability of miR-15a to suppress cancer cell viability and migration is a very important phenomenon for understanding cancer heterogeneity in regard to adapted therapeutic approach development.
format	article
author	Todorova K. Hayrabedyan S.
author_facet	Todorova K. Hayrabedyan S.
author_sort	Todorova K.
title	Mir-15A Reconstitution in Prostate Cancer Cell Line Suppresses Cancer Progression Through Down Regulation of MYB and Androgen Receptor Upregulation
title_short	Mir-15A Reconstitution in Prostate Cancer Cell Line Suppresses Cancer Progression Through Down Regulation of MYB and Androgen Receptor Upregulation
title_full	Mir-15A Reconstitution in Prostate Cancer Cell Line Suppresses Cancer Progression Through Down Regulation of MYB and Androgen Receptor Upregulation
title_fullStr	Mir-15A Reconstitution in Prostate Cancer Cell Line Suppresses Cancer Progression Through Down Regulation of MYB and Androgen Receptor Upregulation
title_full_unstemmed	Mir-15A Reconstitution in Prostate Cancer Cell Line Suppresses Cancer Progression Through Down Regulation of MYB and Androgen Receptor Upregulation
title_sort	mir-15a reconstitution in prostate cancer cell line suppresses cancer progression through down regulation of myb and androgen receptor upregulation
publisher	Sciendo
publishDate	2015
url	https://doaj.org/article/d1644c10e2b943ee882759a2462eacab
work_keys_str_mv	AT todorovak mir15areconstitutioninprostatecancercelllinesuppressescancerprogressionthroughdownregulationofmybandandrogenreceptorupregulation AT hayrabedyans mir15areconstitutioninprostatecancercelllinesuppressescancerprogressionthroughdownregulationofmybandandrogenreceptorupregulation
_version_	1718380520829616128

Mir-15A Reconstitution in Prostate Cancer Cell Line Suppresses Cancer Progression Through Down Regulation of MYB and Androgen Receptor Upregulation

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