Antioxidative nanofullerol prevents intervertebral disk degeneration

Xinlin Yang,1 Li Jin,1 Lu Yao,2,3 Francis H Shen,1 Adam L Shimer,1 Xudong Li11Orthopaedic Research Laboratories, University of Virginia, Charlottesville, VA, USA; 2School of Life Science, Beijing Institute of Technology, Beijing, People's Republic of China; 3Research Institute of Beijing To...

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Autores principales:	Yang X, Jin L, Yao L, Shen FH, Shimer AL, Li X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d16bb9c7de4d470fbc27e98da81390d9
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spelling	oai:doaj.org-article:d16bb9c7de4d470fbc27e98da81390d92021-12-02T00:10:22ZAntioxidative nanofullerol prevents intervertebral disk degeneration1178-2013https://doaj.org/article/d16bb9c7de4d470fbc27e98da81390d92014-05-01T00:00:00Zhttp://www.dovepress.com/antioxidative-nanofullerol-prevents-intervertebral-disk-degeneration-a16859https://doaj.org/toc/1178-2013 Xinlin Yang,1 Li Jin,1 Lu Yao,2,3 Francis H Shen,1 Adam L Shimer,1 Xudong Li11Orthopaedic Research Laboratories, University of Virginia, Charlottesville, VA, USA; 2School of Life Science, Beijing Institute of Technology, Beijing, People's Republic of China; 3Research Institute of Beijing Tongrentang Co., Ltd, Beijing, People's Republic of ChinaAbstract: Compelling evidence suggests that reactive oxygen species (ROS) play a pivotal role in disk degeneration. Fullerol nanoparticles prepared in aqueous solution have been demonstrated to have outstanding ability to scavenge ROS. In this report, in vitro and in vivo models were used to study the efficacy of fullerol in preventing disk degeneration. For in vitro experiments, a pro-oxidant H2O2 or an inflammatory cytokine interleukin (IL)-1β was employed to induce degenerated phenotypes in human nucleus pulposus cells encapsulated in alginate beads, and fullerol was added in the culture medium. For the animal study, an annulus-puncture model with rabbit was created, and fullerol was injected into disks. It was shown that cytotoxicity and cellular ROS level induced by H2O2 were significantly diminished by fullerol. IL-1β -induced nitric oxide generation in culture medium was suppressed by fullerol as well. Gene-profile and biochemical assays showed that fullerol effectively reversed the matrix degradation caused by either H2O2 or IL-1β. The animal study delineated that intradiskal injection of fullerol prevented disk degeneration, increasing water and proteoglycan content and inhibiting ectopic bone formation. These results suggest that antioxidative fullerol may have a potential therapeutic application for disk degeneration.Keywords: free radical, extracellular matrix, intervertebral disk, antioxidant, fullerolYang XJin LYao LShen FHShimer ALLi XDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 2419-2430 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Yang X Jin L Yao L Shen FH Shimer AL Li X Antioxidative nanofullerol prevents intervertebral disk degeneration
description	Xinlin Yang,1 Li Jin,1 Lu Yao,2,3 Francis H Shen,1 Adam L Shimer,1 Xudong Li11Orthopaedic Research Laboratories, University of Virginia, Charlottesville, VA, USA; 2School of Life Science, Beijing Institute of Technology, Beijing, People's Republic of China; 3Research Institute of Beijing Tongrentang Co., Ltd, Beijing, People's Republic of ChinaAbstract: Compelling evidence suggests that reactive oxygen species (ROS) play a pivotal role in disk degeneration. Fullerol nanoparticles prepared in aqueous solution have been demonstrated to have outstanding ability to scavenge ROS. In this report, in vitro and in vivo models were used to study the efficacy of fullerol in preventing disk degeneration. For in vitro experiments, a pro-oxidant H2O2 or an inflammatory cytokine interleukin (IL)-1β was employed to induce degenerated phenotypes in human nucleus pulposus cells encapsulated in alginate beads, and fullerol was added in the culture medium. For the animal study, an annulus-puncture model with rabbit was created, and fullerol was injected into disks. It was shown that cytotoxicity and cellular ROS level induced by H2O2 were significantly diminished by fullerol. IL-1β -induced nitric oxide generation in culture medium was suppressed by fullerol as well. Gene-profile and biochemical assays showed that fullerol effectively reversed the matrix degradation caused by either H2O2 or IL-1β. The animal study delineated that intradiskal injection of fullerol prevented disk degeneration, increasing water and proteoglycan content and inhibiting ectopic bone formation. These results suggest that antioxidative fullerol may have a potential therapeutic application for disk degeneration.Keywords: free radical, extracellular matrix, intervertebral disk, antioxidant, fullerol
format	article
author	Yang X Jin L Yao L Shen FH Shimer AL Li X
author_facet	Yang X Jin L Yao L Shen FH Shimer AL Li X
author_sort	Yang X
title	Antioxidative nanofullerol prevents intervertebral disk degeneration
title_short	Antioxidative nanofullerol prevents intervertebral disk degeneration
title_full	Antioxidative nanofullerol prevents intervertebral disk degeneration
title_fullStr	Antioxidative nanofullerol prevents intervertebral disk degeneration
title_full_unstemmed	Antioxidative nanofullerol prevents intervertebral disk degeneration
title_sort	antioxidative nanofullerol prevents intervertebral disk degeneration
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/d16bb9c7de4d470fbc27e98da81390d9
work_keys_str_mv	AT yangx antioxidativenanofullerolpreventsintervertebraldiskdegeneration AT jinl antioxidativenanofullerolpreventsintervertebraldiskdegeneration AT yaol antioxidativenanofullerolpreventsintervertebraldiskdegeneration AT shenfh antioxidativenanofullerolpreventsintervertebraldiskdegeneration AT shimeral antioxidativenanofullerolpreventsintervertebraldiskdegeneration AT lix antioxidativenanofullerolpreventsintervertebraldiskdegeneration
_version_	1718403892340850688

Antioxidative nanofullerol prevents intervertebral disk degeneration

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