Mechanisms of Cell Entry by dsRNA Viruses: Insights for Efficient Delivery of dsRNA and Tools for Improved RNAi-Based Pest Control

While RNAi is often heralded as a promising new strategy for insect pest control, a major obstacle that still remains is the efficient delivery of dsRNA molecules within the cells of the targeted insects. However, it seems overlooked that dsRNA viruses already have developed efficient strategies for...

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Autores principales:	Luc Swevers, Dimitrios Kontogiannatos, Anna Kolliopoulou, Feifei Ren, Min Feng, Jingchen Sun
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	dsRNA virus entry mechanism arbo-reovirus plant reovirus Cypovirus RNAi Physiology QP1-981
Acceso en línea:	https://doaj.org/article/d177618bbadb49a5b4f01999eed82ecb
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spelling	oai:doaj.org-article:d177618bbadb49a5b4f01999eed82ecb2021-11-11T07:56:33ZMechanisms of Cell Entry by dsRNA Viruses: Insights for Efficient Delivery of dsRNA and Tools for Improved RNAi-Based Pest Control1664-042X10.3389/fphys.2021.749387https://doaj.org/article/d177618bbadb49a5b4f01999eed82ecb2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphys.2021.749387/fullhttps://doaj.org/toc/1664-042XWhile RNAi is often heralded as a promising new strategy for insect pest control, a major obstacle that still remains is the efficient delivery of dsRNA molecules within the cells of the targeted insects. However, it seems overlooked that dsRNA viruses already have developed efficient strategies for transport of dsRNA molecules across tissue barriers and cellular membranes. Besides protecting their dsRNA genomes in a protective shell, dsRNA viruses also display outer capsid layers that incorporate sophisticated mechanisms to disrupt the plasma membrane layer and to translocate core particles (with linear dsRNA genome fragments) within the cytoplasm. Because of the perceived efficiency of the translocation mechanism, it is well worth analyzing in detail the molecular processes that are used to achieve this feat. In this review, the mechanism of cell entry by dsRNA viruses belonging to the Reoviridae family is discussed in detail. Because of the large amount of progress in mammalian versus insect models, the mechanism of infections of reoviruses in mammals (orthoreoviruses, rotaviruses, orbiviruses) will be treated as a point of reference against which infections of reoviruses in insects (orbiviruses in midges, plant viruses in hemipterans, insect-specific cypoviruses in lepidopterans) will be compared. The goal of this discussion is to uncover the basic principles by which dsRNA viruses cross tissue barriers and translocate their cargo to the cellular cytoplasm; such knowledge subsequently can be incorporated into the design of dsRNA virus-based viral-like particles for optimal delivery of RNAi triggers in targeted insect pests.Luc SweversDimitrios KontogiannatosAnna KolliopoulouFeifei RenMin FengJingchen SunFrontiers Media S.A.articledsRNA virusentry mechanismarbo-reovirusplant reovirusCypovirusRNAiPhysiologyQP1-981ENFrontiers in Physiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dsRNA virus entry mechanism arbo-reovirus plant reovirus Cypovirus RNAi Physiology QP1-981
spellingShingle	dsRNA virus entry mechanism arbo-reovirus plant reovirus Cypovirus RNAi Physiology QP1-981 Luc Swevers Dimitrios Kontogiannatos Anna Kolliopoulou Feifei Ren Min Feng Jingchen Sun Mechanisms of Cell Entry by dsRNA Viruses: Insights for Efficient Delivery of dsRNA and Tools for Improved RNAi-Based Pest Control
description	While RNAi is often heralded as a promising new strategy for insect pest control, a major obstacle that still remains is the efficient delivery of dsRNA molecules within the cells of the targeted insects. However, it seems overlooked that dsRNA viruses already have developed efficient strategies for transport of dsRNA molecules across tissue barriers and cellular membranes. Besides protecting their dsRNA genomes in a protective shell, dsRNA viruses also display outer capsid layers that incorporate sophisticated mechanisms to disrupt the plasma membrane layer and to translocate core particles (with linear dsRNA genome fragments) within the cytoplasm. Because of the perceived efficiency of the translocation mechanism, it is well worth analyzing in detail the molecular processes that are used to achieve this feat. In this review, the mechanism of cell entry by dsRNA viruses belonging to the Reoviridae family is discussed in detail. Because of the large amount of progress in mammalian versus insect models, the mechanism of infections of reoviruses in mammals (orthoreoviruses, rotaviruses, orbiviruses) will be treated as a point of reference against which infections of reoviruses in insects (orbiviruses in midges, plant viruses in hemipterans, insect-specific cypoviruses in lepidopterans) will be compared. The goal of this discussion is to uncover the basic principles by which dsRNA viruses cross tissue barriers and translocate their cargo to the cellular cytoplasm; such knowledge subsequently can be incorporated into the design of dsRNA virus-based viral-like particles for optimal delivery of RNAi triggers in targeted insect pests.
format	article
author	Luc Swevers Dimitrios Kontogiannatos Anna Kolliopoulou Feifei Ren Min Feng Jingchen Sun
author_facet	Luc Swevers Dimitrios Kontogiannatos Anna Kolliopoulou Feifei Ren Min Feng Jingchen Sun
author_sort	Luc Swevers
title	Mechanisms of Cell Entry by dsRNA Viruses: Insights for Efficient Delivery of dsRNA and Tools for Improved RNAi-Based Pest Control
title_short	Mechanisms of Cell Entry by dsRNA Viruses: Insights for Efficient Delivery of dsRNA and Tools for Improved RNAi-Based Pest Control
title_full	Mechanisms of Cell Entry by dsRNA Viruses: Insights for Efficient Delivery of dsRNA and Tools for Improved RNAi-Based Pest Control
title_fullStr	Mechanisms of Cell Entry by dsRNA Viruses: Insights for Efficient Delivery of dsRNA and Tools for Improved RNAi-Based Pest Control
title_full_unstemmed	Mechanisms of Cell Entry by dsRNA Viruses: Insights for Efficient Delivery of dsRNA and Tools for Improved RNAi-Based Pest Control
title_sort	mechanisms of cell entry by dsrna viruses: insights for efficient delivery of dsrna and tools for improved rnai-based pest control
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/d177618bbadb49a5b4f01999eed82ecb
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Mechanisms of Cell Entry by dsRNA Viruses: Insights for Efficient Delivery of dsRNA and Tools for Improved RNAi-Based Pest Control

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