Applicability of infectivity assay for the quantification of infectious human adenovirus genotype 5 in UV-irradiated wastewater

The use of infectivity assays in domestic wastewater samples is limited because of the concerns around cytotoxicity to host cells, thus, the UV inactivation efficiency of human adenoviruses (HAdVs) in wastewater remains unclear. In this study, a human adenovirus genotype five (HAdV-5) host cell line...

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Autores principales:	Surapong Rattanakul, Kumiko Oguma
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	adenovirus cytotoxicity hydroxyl radicals infectivity assay uv disinfection Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/d17890624d404bdd8e68b20eb5af786a
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spelling	oai:doaj.org-article:d17890624d404bdd8e68b20eb5af786a2021-11-06T05:45:59ZApplicability of infectivity assay for the quantification of infectious human adenovirus genotype 5 in UV-irradiated wastewater1477-89201996-782910.2166/wh.2020.224https://doaj.org/article/d17890624d404bdd8e68b20eb5af786a2021-02-01T00:00:00Zhttp://jwh.iwaponline.com/content/19/1/67https://doaj.org/toc/1477-8920https://doaj.org/toc/1996-7829The use of infectivity assays in domestic wastewater samples is limited because of the concerns around cytotoxicity to host cells, thus, the UV inactivation efficiency of human adenoviruses (HAdVs) in wastewater remains unclear. In this study, a human adenovirus genotype five (HAdV-5) host cell line (A549 cells) was incubated with wastewater at varied dilutions from 1:1.5 to 1:4 (the ratio of wastewater to a mixture of wastewater and the cell culture medium) and the cytotoxicity was assessed by the cell morphology and viability. No change was observed in either cell viability or morphology in comparison with control samples, even at lowest dilution of 1:1.5, indicating the dilution allowed infectivity assays. The minimal degree of dilution to avoid cytotoxicity may differ with different water matrix. Consequently, the technique was applied to quantify spiked HAdV-5 after the UV disinfection of wastewater. A significant increase in UV disinfection efficiency was noted in wastewater and hydroxyl radicals (OH•) produced by the photosensitization of dissolved organic matter were suggested to be responsible for the enhancement. This study indicated that dilution can be a simple solution to avoid cytotoxicity, and UV inactivation may be enhanced in wastewater due to OH• radicals produced by UV radiation.Surapong RattanakulKumiko OgumaIWA Publishingarticleadenoviruscytotoxicityhydroxyl radicalsinfectivity assayuv disinfectionPublic aspects of medicineRA1-1270ENJournal of Water and Health, Vol 19, Iss 1, Pp 67-78 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adenovirus cytotoxicity hydroxyl radicals infectivity assay uv disinfection Public aspects of medicine RA1-1270
spellingShingle	adenovirus cytotoxicity hydroxyl radicals infectivity assay uv disinfection Public aspects of medicine RA1-1270 Surapong Rattanakul Kumiko Oguma Applicability of infectivity assay for the quantification of infectious human adenovirus genotype 5 in UV-irradiated wastewater
description	The use of infectivity assays in domestic wastewater samples is limited because of the concerns around cytotoxicity to host cells, thus, the UV inactivation efficiency of human adenoviruses (HAdVs) in wastewater remains unclear. In this study, a human adenovirus genotype five (HAdV-5) host cell line (A549 cells) was incubated with wastewater at varied dilutions from 1:1.5 to 1:4 (the ratio of wastewater to a mixture of wastewater and the cell culture medium) and the cytotoxicity was assessed by the cell morphology and viability. No change was observed in either cell viability or morphology in comparison with control samples, even at lowest dilution of 1:1.5, indicating the dilution allowed infectivity assays. The minimal degree of dilution to avoid cytotoxicity may differ with different water matrix. Consequently, the technique was applied to quantify spiked HAdV-5 after the UV disinfection of wastewater. A significant increase in UV disinfection efficiency was noted in wastewater and hydroxyl radicals (OH•) produced by the photosensitization of dissolved organic matter were suggested to be responsible for the enhancement. This study indicated that dilution can be a simple solution to avoid cytotoxicity, and UV inactivation may be enhanced in wastewater due to OH• radicals produced by UV radiation.
format	article
author	Surapong Rattanakul Kumiko Oguma
author_facet	Surapong Rattanakul Kumiko Oguma
author_sort	Surapong Rattanakul
title	Applicability of infectivity assay for the quantification of infectious human adenovirus genotype 5 in UV-irradiated wastewater
title_short	Applicability of infectivity assay for the quantification of infectious human adenovirus genotype 5 in UV-irradiated wastewater
title_full	Applicability of infectivity assay for the quantification of infectious human adenovirus genotype 5 in UV-irradiated wastewater
title_fullStr	Applicability of infectivity assay for the quantification of infectious human adenovirus genotype 5 in UV-irradiated wastewater
title_full_unstemmed	Applicability of infectivity assay for the quantification of infectious human adenovirus genotype 5 in UV-irradiated wastewater
title_sort	applicability of infectivity assay for the quantification of infectious human adenovirus genotype 5 in uv-irradiated wastewater
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/d17890624d404bdd8e68b20eb5af786a
work_keys_str_mv	AT surapongrattanakul applicabilityofinfectivityassayforthequantificationofinfectioushumanadenovirusgenotype5inuvirradiatedwastewater AT kumikooguma applicabilityofinfectivityassayforthequantificationofinfectioushumanadenovirusgenotype5inuvirradiatedwastewater
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Applicability of infectivity assay for the quantification of infectious human adenovirus genotype 5 in UV-irradiated wastewater

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