Immunohistochemical detection of TAS2R38 protein in human taste cells.

The sense of taste plays an important role in the evaluation of the nutrient composition of consumed food. Bitter taste in particular is believed to serve a warning function against the ingestion of poisonous substances. In the past years enormous progress was made in the characterization of bitter...

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Autores principales:	Maik Behrens, Stephan Born, Ulrike Redel, Nadine Voigt, Vanessa Schuh, Jan-Dirk Raguse, Wolfgang Meyerhof
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d180841136ee4ad4a093ac0a937ab60a
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spelling	oai:doaj.org-article:d180841136ee4ad4a093ac0a937ab60a2021-11-18T07:13:13ZImmunohistochemical detection of TAS2R38 protein in human taste cells.1932-620310.1371/journal.pone.0040304https://doaj.org/article/d180841136ee4ad4a093ac0a937ab60a2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22792271/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The sense of taste plays an important role in the evaluation of the nutrient composition of consumed food. Bitter taste in particular is believed to serve a warning function against the ingestion of poisonous substances. In the past years enormous progress was made in the characterization of bitter taste receptors, including their gene expression patterns, pharmacological features and presumed physiological roles in gustatory as well as in non-gustatory tissues. However, due to a lack in TAS2R-specifc antibodies the localization of receptor proteins within gustatory tissues has never been analyzed. In the present study we have screened a panel of commercially available antisera raised against human bitter taste receptors by immunocytochemical experiments. One of these antisera was found to be highly specific for the human bitter taste receptor TAS2R38. We further demonstrate that this antibody is able to detect heterologously expressed TAS2R38 protein on Western blots. The antiserum is, however, not able to interfere significantly with TAS2R38 function in cell based calcium imaging analyses. Most importantly, we were able to demonstrate the presence of TAS2R38 protein in human gustatory papillae. Using double immunofluorescence we show that TAS2R38-positive cells form a subpopulation of PLCbeta2 expressing cells. On a subcellular level the localization of this bitter taste receptor is neither restricted to the cell surface nor particularly enriched at the level of the microvilli protruding into the pore region of the taste buds, but rather evenly distributed over the entire cell body.Maik BehrensStephan BornUlrike RedelNadine VoigtVanessa SchuhJan-Dirk RaguseWolfgang MeyerhofPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e40304 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maik Behrens Stephan Born Ulrike Redel Nadine Voigt Vanessa Schuh Jan-Dirk Raguse Wolfgang Meyerhof Immunohistochemical detection of TAS2R38 protein in human taste cells.
description	The sense of taste plays an important role in the evaluation of the nutrient composition of consumed food. Bitter taste in particular is believed to serve a warning function against the ingestion of poisonous substances. In the past years enormous progress was made in the characterization of bitter taste receptors, including their gene expression patterns, pharmacological features and presumed physiological roles in gustatory as well as in non-gustatory tissues. However, due to a lack in TAS2R-specifc antibodies the localization of receptor proteins within gustatory tissues has never been analyzed. In the present study we have screened a panel of commercially available antisera raised against human bitter taste receptors by immunocytochemical experiments. One of these antisera was found to be highly specific for the human bitter taste receptor TAS2R38. We further demonstrate that this antibody is able to detect heterologously expressed TAS2R38 protein on Western blots. The antiserum is, however, not able to interfere significantly with TAS2R38 function in cell based calcium imaging analyses. Most importantly, we were able to demonstrate the presence of TAS2R38 protein in human gustatory papillae. Using double immunofluorescence we show that TAS2R38-positive cells form a subpopulation of PLCbeta2 expressing cells. On a subcellular level the localization of this bitter taste receptor is neither restricted to the cell surface nor particularly enriched at the level of the microvilli protruding into the pore region of the taste buds, but rather evenly distributed over the entire cell body.
format	article
author	Maik Behrens Stephan Born Ulrike Redel Nadine Voigt Vanessa Schuh Jan-Dirk Raguse Wolfgang Meyerhof
author_facet	Maik Behrens Stephan Born Ulrike Redel Nadine Voigt Vanessa Schuh Jan-Dirk Raguse Wolfgang Meyerhof
author_sort	Maik Behrens
title	Immunohistochemical detection of TAS2R38 protein in human taste cells.
title_short	Immunohistochemical detection of TAS2R38 protein in human taste cells.
title_full	Immunohistochemical detection of TAS2R38 protein in human taste cells.
title_fullStr	Immunohistochemical detection of TAS2R38 protein in human taste cells.
title_full_unstemmed	Immunohistochemical detection of TAS2R38 protein in human taste cells.
title_sort	immunohistochemical detection of tas2r38 protein in human taste cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/d180841136ee4ad4a093ac0a937ab60a
work_keys_str_mv	AT maikbehrens immunohistochemicaldetectionoftas2r38proteininhumantastecells AT stephanborn immunohistochemicaldetectionoftas2r38proteininhumantastecells AT ulrikeredel immunohistochemicaldetectionoftas2r38proteininhumantastecells AT nadinevoigt immunohistochemicaldetectionoftas2r38proteininhumantastecells AT vanessaschuh immunohistochemicaldetectionoftas2r38proteininhumantastecells AT jandirkraguse immunohistochemicaldetectionoftas2r38proteininhumantastecells AT wolfgangmeyerhof immunohistochemicaldetectionoftas2r38proteininhumantastecells
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Immunohistochemical detection of TAS2R38 protein in human taste cells.

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