Effects of size and surface of zinc oxide and aluminum-doped zinc oxide nanoparticles on cell viability inferred by proteomic analyses

Chih-Hong Pan,1,2,* Wen-Te Liu,3,4,* Mauo-Ying Bien,4,5 I-Chan Lin,6 Ta-Chih Hsiao,7 Chih-Ming Ma,8 Ching-Huang Lai,2 Mei-Chieh Chen,9 Kai-Jen Chuang,10,11 Hsiao-Chi Chuang3,4 On behalf of the Taiwan CardioPulmonary Research (T-CPR) Group 1Institute of Labor, Occupational Safety and Health, Minis...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Pan CH, Liu WT, Bien MY, Lin IC, Hsiao TC, Ma CM, Lai CH, Chen MC, Chuang KJ, Chuang HC
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d18092059a054acc857e5ef153dd1dd7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d18092059a054acc857e5ef153dd1dd7
record_format	dspace
spelling	oai:doaj.org-article:d18092059a054acc857e5ef153dd1dd72021-12-02T02:10:31ZEffects of size and surface of zinc oxide and aluminum-doped zinc oxide nanoparticles on cell viability inferred by proteomic analyses1178-2013https://doaj.org/article/d18092059a054acc857e5ef153dd1dd72014-08-01T00:00:00Zhttp://www.dovepress.com/effects-of-size-and-surface-of-zinc-oxide-and-aluminum-doped-zinc-oxid-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Chih-Hong Pan,1,2,* Wen-Te Liu,3,4,* Mauo-Ying Bien,4,5 I-Chan Lin,6 Ta-Chih Hsiao,7 Chih-Ming Ma,8 Ching-Huang Lai,2 Mei-Chieh Chen,9 Kai-Jen Chuang,10,11 Hsiao-Chi Chuang3,4 On behalf of the Taiwan CardioPulmonary Research (T-CPR) Group 1Institute of Labor, Occupational Safety and Health, Ministry of Labor, 2School of Public Health, National Defense Medical Center, 3Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho Hospital, 4School of Respiratory Therapy, College of Medicine, 5Division of Pulmonary Medicine, Department of Internal Medicine, Taipei Medical University Hospital, 6Department of Ophthalmology, Shuang Ho Hospital, Taipei Medical University, Taipei, 7Graduate Institute of Environmental Engineering, National Central University, Taoyuan, 8Department of Cosmetic Application and Management, St Mary’s Junior College of Medicine, Nursing and Management, Sanxing, 9Department of Microbiology and Immunology, College of Medicine, 10Department of Public Health, School of Medicine, College of Medicine, 11School of Public Health, College of Public Health and Nutrition, Taipei Medical University, Taipei, Taiwan *These authors contributed equally to this work Abstract: Although the health effects of zinc oxide nanoparticles (ZnONPs) on the respiratory system have been reported, the fate, potential toxicity, and mechanisms in biological cells of these particles, as related to particle size and surface characteristics, have not been well elucidated. To determine the physicochemical properties of ZnONPs that govern cytotoxicity, we investigated the effects of size, electronic properties, zinc concentration, and pH on cell viability using human alveolar-basal epithelial A549 cells as a model. We observed that a 2-hour or longer exposure to ZnONPs induced changes in cell viability. The alteration in cell viability was associated with the zeta potentials and pH values of the ZnONPs. Proteomic profiling of A549 exposed to ZnONPs for 2 and 4 hours was used to determine the biological mechanisms of ZnONP toxicity. p53-pathway activation was the core mechanism regulating cell viability in response to particle size. Activation of the Wnt and TGFß signaling pathways was also important in the cellular response to ZnONPs of different sizes. The cadherin and Wnt signaling pathways were important cellular mechanisms triggered by surface differences. These results suggested that the size and surface characteristics of ZnONPs might play an important role in their observed cytotoxicity. This approach facilitates the design of more comprehensive systems for the evaluation of nanoparticles. Keywords: aluminium-doped zinc oxide, nanoparticle, pH, toxicology, WNT pathway, zeta potentialPan CHLiu WTBien MYLin ICHsiao TCMa CMLai CHChen MCChuang KJChuang HCDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 3631-3643 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Pan CH Liu WT Bien MY Lin IC Hsiao TC Ma CM Lai CH Chen MC Chuang KJ Chuang HC Effects of size and surface of zinc oxide and aluminum-doped zinc oxide nanoparticles on cell viability inferred by proteomic analyses
description	Chih-Hong Pan,1,2,* Wen-Te Liu,3,4,* Mauo-Ying Bien,4,5 I-Chan Lin,6 Ta-Chih Hsiao,7 Chih-Ming Ma,8 Ching-Huang Lai,2 Mei-Chieh Chen,9 Kai-Jen Chuang,10,11 Hsiao-Chi Chuang3,4 On behalf of the Taiwan CardioPulmonary Research (T-CPR) Group 1Institute of Labor, Occupational Safety and Health, Ministry of Labor, 2School of Public Health, National Defense Medical Center, 3Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho Hospital, 4School of Respiratory Therapy, College of Medicine, 5Division of Pulmonary Medicine, Department of Internal Medicine, Taipei Medical University Hospital, 6Department of Ophthalmology, Shuang Ho Hospital, Taipei Medical University, Taipei, 7Graduate Institute of Environmental Engineering, National Central University, Taoyuan, 8Department of Cosmetic Application and Management, St Mary’s Junior College of Medicine, Nursing and Management, Sanxing, 9Department of Microbiology and Immunology, College of Medicine, 10Department of Public Health, School of Medicine, College of Medicine, 11School of Public Health, College of Public Health and Nutrition, Taipei Medical University, Taipei, Taiwan *These authors contributed equally to this work Abstract: Although the health effects of zinc oxide nanoparticles (ZnONPs) on the respiratory system have been reported, the fate, potential toxicity, and mechanisms in biological cells of these particles, as related to particle size and surface characteristics, have not been well elucidated. To determine the physicochemical properties of ZnONPs that govern cytotoxicity, we investigated the effects of size, electronic properties, zinc concentration, and pH on cell viability using human alveolar-basal epithelial A549 cells as a model. We observed that a 2-hour or longer exposure to ZnONPs induced changes in cell viability. The alteration in cell viability was associated with the zeta potentials and pH values of the ZnONPs. Proteomic profiling of A549 exposed to ZnONPs for 2 and 4 hours was used to determine the biological mechanisms of ZnONP toxicity. p53-pathway activation was the core mechanism regulating cell viability in response to particle size. Activation of the Wnt and TGFß signaling pathways was also important in the cellular response to ZnONPs of different sizes. The cadherin and Wnt signaling pathways were important cellular mechanisms triggered by surface differences. These results suggested that the size and surface characteristics of ZnONPs might play an important role in their observed cytotoxicity. This approach facilitates the design of more comprehensive systems for the evaluation of nanoparticles. Keywords: aluminium-doped zinc oxide, nanoparticle, pH, toxicology, WNT pathway, zeta potential
format	article
author	Pan CH Liu WT Bien MY Lin IC Hsiao TC Ma CM Lai CH Chen MC Chuang KJ Chuang HC
author_facet	Pan CH Liu WT Bien MY Lin IC Hsiao TC Ma CM Lai CH Chen MC Chuang KJ Chuang HC
author_sort	Pan CH
title	Effects of size and surface of zinc oxide and aluminum-doped zinc oxide nanoparticles on cell viability inferred by proteomic analyses
title_short	Effects of size and surface of zinc oxide and aluminum-doped zinc oxide nanoparticles on cell viability inferred by proteomic analyses
title_full	Effects of size and surface of zinc oxide and aluminum-doped zinc oxide nanoparticles on cell viability inferred by proteomic analyses
title_fullStr	Effects of size and surface of zinc oxide and aluminum-doped zinc oxide nanoparticles on cell viability inferred by proteomic analyses
title_full_unstemmed	Effects of size and surface of zinc oxide and aluminum-doped zinc oxide nanoparticles on cell viability inferred by proteomic analyses
title_sort	effects of size and surface of zinc oxide and aluminum-doped zinc oxide nanoparticles on cell viability inferred by proteomic analyses
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/d18092059a054acc857e5ef153dd1dd7
work_keys_str_mv	AT panch effectsofsizeandsurfaceofzincoxideandaluminumdopedzincoxidenanoparticlesoncellviabilityinferredbyproteomicanalyses AT liuwt effectsofsizeandsurfaceofzincoxideandaluminumdopedzincoxidenanoparticlesoncellviabilityinferredbyproteomicanalyses AT bienmy effectsofsizeandsurfaceofzincoxideandaluminumdopedzincoxidenanoparticlesoncellviabilityinferredbyproteomicanalyses AT linic effectsofsizeandsurfaceofzincoxideandaluminumdopedzincoxidenanoparticlesoncellviabilityinferredbyproteomicanalyses AT hsiaotc effectsofsizeandsurfaceofzincoxideandaluminumdopedzincoxidenanoparticlesoncellviabilityinferredbyproteomicanalyses AT macm effectsofsizeandsurfaceofzincoxideandaluminumdopedzincoxidenanoparticlesoncellviabilityinferredbyproteomicanalyses AT laich effectsofsizeandsurfaceofzincoxideandaluminumdopedzincoxidenanoparticlesoncellviabilityinferredbyproteomicanalyses AT chenmc effectsofsizeandsurfaceofzincoxideandaluminumdopedzincoxidenanoparticlesoncellviabilityinferredbyproteomicanalyses AT chuangkj effectsofsizeandsurfaceofzincoxideandaluminumdopedzincoxidenanoparticlesoncellviabilityinferredbyproteomicanalyses AT chuanghc effectsofsizeandsurfaceofzincoxideandaluminumdopedzincoxidenanoparticlesoncellviabilityinferredbyproteomicanalyses
_version_	1718402679568334848

Effects of size and surface of zinc oxide and aluminum-doped zinc oxide nanoparticles on cell viability inferred by proteomic analyses

Ejemplares similares