Ligand-independent traffic of Notch buffers activated Armadillo in Drosophila.

Notch receptors act as ligand-dependent membrane-tethered transcription factors with a prominent role in binary cell fate decisions during development, which is conserved across species. In addition there is increasing evidence for other functions of Notch, particularly in connection with Wnt signal...

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Autores principales:	Phil G T Sanders, Silvia Muñoz-Descalzo, Tina Balayo, Frederik Wirtz-Peitz, Penelope Hayward, Alfonso Martinez Arias
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d18f846e588748a0be3aace58e765a50
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spelling	oai:doaj.org-article:d18f846e588748a0be3aace58e765a502021-11-25T05:34:04ZLigand-independent traffic of Notch buffers activated Armadillo in Drosophila.1544-91731545-788510.1371/journal.pbio.1000169https://doaj.org/article/d18f846e588748a0be3aace58e765a502009-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19668359/pdf/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Notch receptors act as ligand-dependent membrane-tethered transcription factors with a prominent role in binary cell fate decisions during development, which is conserved across species. In addition there is increasing evidence for other functions of Notch, particularly in connection with Wnt signalling: Notch is able to modulate the activity of Armadillo/ss-catenin, the effector of Wnt signalling, in a manner that is independent of its transcriptional activity. Here we explore the mechanism of this interaction in the epithelium of the Drosophila imaginal discs and find that it is mediated by the ligand-independent endocytosis and traffic of the Notch receptor. Our results show that Notch associates with Armadillo near the adherens junctions and that it is rapidly endocytosed promoting the traffic of an activated form of Armadillo into endosomal compartments, where it may be degraded. As Notch has the ability to interact with and downregulate activated forms of Armadillo, it is possible that in vivo Notch regulates the transcriptionally competent pool of Armadillo. These interactions reveal a previously unknown activity of Notch, which serves to buffer the function of activated Armadillo and might underlie some of its transcription-independent effects.Phil G T SandersSilvia Muñoz-DescalzoTina BalayoFrederik Wirtz-PeitzPenelope HaywardAlfonso Martinez AriasPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 7, Iss 8, p e1000169 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Phil G T Sanders Silvia Muñoz-Descalzo Tina Balayo Frederik Wirtz-Peitz Penelope Hayward Alfonso Martinez Arias Ligand-independent traffic of Notch buffers activated Armadillo in Drosophila.
description	Notch receptors act as ligand-dependent membrane-tethered transcription factors with a prominent role in binary cell fate decisions during development, which is conserved across species. In addition there is increasing evidence for other functions of Notch, particularly in connection with Wnt signalling: Notch is able to modulate the activity of Armadillo/ss-catenin, the effector of Wnt signalling, in a manner that is independent of its transcriptional activity. Here we explore the mechanism of this interaction in the epithelium of the Drosophila imaginal discs and find that it is mediated by the ligand-independent endocytosis and traffic of the Notch receptor. Our results show that Notch associates with Armadillo near the adherens junctions and that it is rapidly endocytosed promoting the traffic of an activated form of Armadillo into endosomal compartments, where it may be degraded. As Notch has the ability to interact with and downregulate activated forms of Armadillo, it is possible that in vivo Notch regulates the transcriptionally competent pool of Armadillo. These interactions reveal a previously unknown activity of Notch, which serves to buffer the function of activated Armadillo and might underlie some of its transcription-independent effects.
format	article
author	Phil G T Sanders Silvia Muñoz-Descalzo Tina Balayo Frederik Wirtz-Peitz Penelope Hayward Alfonso Martinez Arias
author_facet	Phil G T Sanders Silvia Muñoz-Descalzo Tina Balayo Frederik Wirtz-Peitz Penelope Hayward Alfonso Martinez Arias
author_sort	Phil G T Sanders
title	Ligand-independent traffic of Notch buffers activated Armadillo in Drosophila.
title_short	Ligand-independent traffic of Notch buffers activated Armadillo in Drosophila.
title_full	Ligand-independent traffic of Notch buffers activated Armadillo in Drosophila.
title_fullStr	Ligand-independent traffic of Notch buffers activated Armadillo in Drosophila.
title_full_unstemmed	Ligand-independent traffic of Notch buffers activated Armadillo in Drosophila.
title_sort	ligand-independent traffic of notch buffers activated armadillo in drosophila.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/d18f846e588748a0be3aace58e765a50
work_keys_str_mv	AT philgtsanders ligandindependenttrafficofnotchbuffersactivatedarmadilloindrosophila AT silviamunozdescalzo ligandindependenttrafficofnotchbuffersactivatedarmadilloindrosophila AT tinabalayo ligandindependenttrafficofnotchbuffersactivatedarmadilloindrosophila AT frederikwirtzpeitz ligandindependenttrafficofnotchbuffersactivatedarmadilloindrosophila AT penelopehayward ligandindependenttrafficofnotchbuffersactivatedarmadilloindrosophila AT alfonsomartinezarias ligandindependenttrafficofnotchbuffersactivatedarmadilloindrosophila
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Ligand-independent traffic of Notch buffers activated Armadillo in Drosophila.

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