Passiflora quadrangularis L. PREVENTS EXPERIMENTAL HYPERTENSION AND VASCULAR REMODELLING IN RATS EXPOSED TO NITRIC OXIDE DEFICIT

ABSTRACT Background: Passiflora quadrangularis L. is among the species used in Colombian folk medicine for hypertension, but until now it has not been studied in experimental models. Objectives : To assess the capacity of P. quadrangularis L. EtOH extract to prevent the hypertension and vascular r...

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Autores principales:	Lesly L. Bareño, Pilar Puebla, Carlos M. Guerra, Arturo San Feliciano, Gustavo Isaza, Mario F. Guerrero
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia
Materias:	Isolated organ hypertension L-NAME Passiflora quadrangularis L. vascular remodelling Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/d19935d08fe547938852cf962396ff2b
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spelling	oai:doaj.org-article:d19935d08fe547938852cf962396ff2b2021-12-02T04:41:15ZPassiflora quadrangularis L. PREVENTS EXPERIMENTAL HYPERTENSION AND VASCULAR REMODELLING IN RATS EXPOSED TO NITRIC OXIDE DEFICIT0121-400410.17533/udea.vitae.v24n4a04https://doaj.org/article/d19935d08fe547938852cf962396ff2bhttp://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0121-40042017000300186&lng=en&tlng=enhttps://doaj.org/toc/0121-4004ABSTRACT Background: Passiflora quadrangularis L. is among the species used in Colombian folk medicine for hypertension, but until now it has not been studied in experimental models. Objectives : To assess the capacity of P. quadrangularis L. EtOH extract to prevent the hypertension and vascular remodelling induced by nitric oxide (NO) deficit in Wistar rats. Methods: The nitric oxide (NO) synthase inhibitor L-NAME (10 mg/kg, i.p (intraperitoneal), every 48h) was administered for seven weeks to the following groups of rats: P. quadrangularis L.75, 150 and 300 mg/kg/d, p.o. (oral route); enalapril as reference agent, 10 mg/kg/d, p.o. and vehicle as control (mixture of propylene glycol 10%, glycerine 10% and polysorbate 2%). Arterial blood pressure (BP) and heart rate (HR) were measured twice a week. After sacrifice, the aortic rings were isolated, contraction was triggered with phenylephrine (PE 10-6 M) and then the relaxant response achieved with cumulative concentrations of acetylcholine (ACh, 10-10 - 10-5 M) or sodium nitroprusside (SNP, 10-10 - 10-5 M) was assessed. Histopathologic measures of thickness/lumen ratio from both the left ventricle and aorta walls, as well as phytochemical screening, were also performed. Results: As for enalapril, all doses of P. quadrangularis L. prevented the hypertension induced by L-NAME (122±1.2 versus 155±1.3 mmHg at seventh week). P. quadrangularis L. significantly increased the relaxant effect induced by ACh in isolated aorta and decreased the thickness/lumen ratio of aorta wall specimens. Conclusions: P. quadrangularis L. prevents experimental hypertension induced in rats with nitric oxide deficits improving the endothelium vasodilatation response and protecting against vascular remodelling.Lesly L. BareñoPilar PueblaCarlos M. GuerraArturo San FelicianoGustavo IsazaMario F. GuerreroUniversidad de AntioquiaarticleIsolated organhypertensionL-NAMEPassiflora quadrangularis L.vascular remodellingFood processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 24, Iss 3, Pp 186-195
institution	DOAJ
collection	DOAJ
language	EN
topic	Isolated organ hypertension L-NAME Passiflora quadrangularis L. vascular remodelling Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	Isolated organ hypertension L-NAME Passiflora quadrangularis L. vascular remodelling Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Lesly L. Bareño Pilar Puebla Carlos M. Guerra Arturo San Feliciano Gustavo Isaza Mario F. Guerrero Passiflora quadrangularis L. PREVENTS EXPERIMENTAL HYPERTENSION AND VASCULAR REMODELLING IN RATS EXPOSED TO NITRIC OXIDE DEFICIT
description	ABSTRACT Background: Passiflora quadrangularis L. is among the species used in Colombian folk medicine for hypertension, but until now it has not been studied in experimental models. Objectives : To assess the capacity of P. quadrangularis L. EtOH extract to prevent the hypertension and vascular remodelling induced by nitric oxide (NO) deficit in Wistar rats. Methods: The nitric oxide (NO) synthase inhibitor L-NAME (10 mg/kg, i.p (intraperitoneal), every 48h) was administered for seven weeks to the following groups of rats: P. quadrangularis L.75, 150 and 300 mg/kg/d, p.o. (oral route); enalapril as reference agent, 10 mg/kg/d, p.o. and vehicle as control (mixture of propylene glycol 10%, glycerine 10% and polysorbate 2%). Arterial blood pressure (BP) and heart rate (HR) were measured twice a week. After sacrifice, the aortic rings were isolated, contraction was triggered with phenylephrine (PE 10-6 M) and then the relaxant response achieved with cumulative concentrations of acetylcholine (ACh, 10-10 - 10-5 M) or sodium nitroprusside (SNP, 10-10 - 10-5 M) was assessed. Histopathologic measures of thickness/lumen ratio from both the left ventricle and aorta walls, as well as phytochemical screening, were also performed. Results: As for enalapril, all doses of P. quadrangularis L. prevented the hypertension induced by L-NAME (122±1.2 versus 155±1.3 mmHg at seventh week). P. quadrangularis L. significantly increased the relaxant effect induced by ACh in isolated aorta and decreased the thickness/lumen ratio of aorta wall specimens. Conclusions: P. quadrangularis L. prevents experimental hypertension induced in rats with nitric oxide deficits improving the endothelium vasodilatation response and protecting against vascular remodelling.
format	article
author	Lesly L. Bareño Pilar Puebla Carlos M. Guerra Arturo San Feliciano Gustavo Isaza Mario F. Guerrero
author_facet	Lesly L. Bareño Pilar Puebla Carlos M. Guerra Arturo San Feliciano Gustavo Isaza Mario F. Guerrero
author_sort	Lesly L. Bareño
title	Passiflora quadrangularis L. PREVENTS EXPERIMENTAL HYPERTENSION AND VASCULAR REMODELLING IN RATS EXPOSED TO NITRIC OXIDE DEFICIT
title_short	Passiflora quadrangularis L. PREVENTS EXPERIMENTAL HYPERTENSION AND VASCULAR REMODELLING IN RATS EXPOSED TO NITRIC OXIDE DEFICIT
title_full	Passiflora quadrangularis L. PREVENTS EXPERIMENTAL HYPERTENSION AND VASCULAR REMODELLING IN RATS EXPOSED TO NITRIC OXIDE DEFICIT
title_fullStr	Passiflora quadrangularis L. PREVENTS EXPERIMENTAL HYPERTENSION AND VASCULAR REMODELLING IN RATS EXPOSED TO NITRIC OXIDE DEFICIT
title_full_unstemmed	Passiflora quadrangularis L. PREVENTS EXPERIMENTAL HYPERTENSION AND VASCULAR REMODELLING IN RATS EXPOSED TO NITRIC OXIDE DEFICIT
title_sort	passiflora quadrangularis l. prevents experimental hypertension and vascular remodelling in rats exposed to nitric oxide deficit
publisher	Universidad de Antioquia
url	https://doaj.org/article/d19935d08fe547938852cf962396ff2b
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Passiflora quadrangularis L. PREVENTS EXPERIMENTAL HYPERTENSION AND VASCULAR REMODELLING IN RATS EXPOSED TO NITRIC OXIDE DEFICIT

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