Identification and functional demonstration of miRNAs in the fungus Cryptococcus neoformans.

microRNAs (miRNAs), endogenous posttranscriptional repressors by base-pairing of their cognate mRNAs in plants and animals, have mostly been thought lost in the kingdom of fungi. Here, we report the identification of miRNAs from the fungus Cryptococcus neoformans. With bioinformatics and Northern bl...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nan Jiang, Yaping Yang, Guilhem Janbon, Jiao Pan, Xudong Zhu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d19b95a655fd46f9bc0abbb4b9a4a86a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d19b95a655fd46f9bc0abbb4b9a4a86a
record_format	dspace
spelling	oai:doaj.org-article:d19b95a655fd46f9bc0abbb4b9a4a86a2021-11-18T08:03:38ZIdentification and functional demonstration of miRNAs in the fungus Cryptococcus neoformans.1932-620310.1371/journal.pone.0052734https://doaj.org/article/d19b95a655fd46f9bc0abbb4b9a4a86a2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23300755/?tool=EBIhttps://doaj.org/toc/1932-6203microRNAs (miRNAs), endogenous posttranscriptional repressors by base-pairing of their cognate mRNAs in plants and animals, have mostly been thought lost in the kingdom of fungi. Here, we report the identification of miRNAs from the fungus Cryptococcus neoformans. With bioinformatics and Northern blotting approaches, we found that these miRNAs and their hairpin precursors were present in this fungus. The size of miR1 and miR2 is 22 nt and 18 nt, respectively. The precursors are about ∼70 nt in length that is close to mammalian pre-miRNAs. Characteristic features of miRNAs are also found in miR1/2. We demonstrated that the identified miRNAs, miR1 and miR2, caused transgene silencing via the canonical RNAi pathway. Bioinformantics analysis helps to reveal a number of identical sequences of the miR1/2 in transposable elements (TEs) and pseudogenes, prompting us to think that fungal miRNAs might be involved in the regulation of the activity of transposons and the expression of pseudogenes. This study identified functional miRNAs in C. neoformans, and sheds light on the diversity and evolutionary origin of eukaryotic miRNAs.Nan JiangYaping YangGuilhem JanbonJiao PanXudong ZhuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e52734 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nan Jiang Yaping Yang Guilhem Janbon Jiao Pan Xudong Zhu Identification and functional demonstration of miRNAs in the fungus Cryptococcus neoformans.
description	microRNAs (miRNAs), endogenous posttranscriptional repressors by base-pairing of their cognate mRNAs in plants and animals, have mostly been thought lost in the kingdom of fungi. Here, we report the identification of miRNAs from the fungus Cryptococcus neoformans. With bioinformatics and Northern blotting approaches, we found that these miRNAs and their hairpin precursors were present in this fungus. The size of miR1 and miR2 is 22 nt and 18 nt, respectively. The precursors are about ∼70 nt in length that is close to mammalian pre-miRNAs. Characteristic features of miRNAs are also found in miR1/2. We demonstrated that the identified miRNAs, miR1 and miR2, caused transgene silencing via the canonical RNAi pathway. Bioinformantics analysis helps to reveal a number of identical sequences of the miR1/2 in transposable elements (TEs) and pseudogenes, prompting us to think that fungal miRNAs might be involved in the regulation of the activity of transposons and the expression of pseudogenes. This study identified functional miRNAs in C. neoformans, and sheds light on the diversity and evolutionary origin of eukaryotic miRNAs.
format	article
author	Nan Jiang Yaping Yang Guilhem Janbon Jiao Pan Xudong Zhu
author_facet	Nan Jiang Yaping Yang Guilhem Janbon Jiao Pan Xudong Zhu
author_sort	Nan Jiang
title	Identification and functional demonstration of miRNAs in the fungus Cryptococcus neoformans.
title_short	Identification and functional demonstration of miRNAs in the fungus Cryptococcus neoformans.
title_full	Identification and functional demonstration of miRNAs in the fungus Cryptococcus neoformans.
title_fullStr	Identification and functional demonstration of miRNAs in the fungus Cryptococcus neoformans.
title_full_unstemmed	Identification and functional demonstration of miRNAs in the fungus Cryptococcus neoformans.
title_sort	identification and functional demonstration of mirnas in the fungus cryptococcus neoformans.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/d19b95a655fd46f9bc0abbb4b9a4a86a
work_keys_str_mv	AT nanjiang identificationandfunctionaldemonstrationofmirnasinthefunguscryptococcusneoformans AT yapingyang identificationandfunctionaldemonstrationofmirnasinthefunguscryptococcusneoformans AT guilhemjanbon identificationandfunctionaldemonstrationofmirnasinthefunguscryptococcusneoformans AT jiaopan identificationandfunctionaldemonstrationofmirnasinthefunguscryptococcusneoformans AT xudongzhu identificationandfunctionaldemonstrationofmirnasinthefunguscryptococcusneoformans
_version_	1718422570675470336

Identification and functional demonstration of miRNAs in the fungus Cryptococcus neoformans.

Ejemplares similares