New Alternative Mixtures of Cryoprotectants for Equine Immature Oocyte Vitrification

Equine oocyte vitrification would benefit the growing in vitro embryo production programs, but further optimization of the protocol is necessary to reach clinical efficiency. Therefore, we aimed to perform a direct comparison of non-permeating and permeating cryoprotective agents (CPAs) during the v...

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Autores principales:	Daniel Angel-Velez, Tine De Coster, Nima Azari-Dolatabad, Andrea Fernandez-Montoro, Camilla Benedetti, Osvaldo Bogado Pascottini, Henri Woelders, Ann Van Soom, Katrien Smits
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cryoprotective agents equine ICSI oocyte vitrification warming Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/d1a2155f14ce437fa5f3fd91f5c4915f
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spelling	oai:doaj.org-article:d1a2155f14ce437fa5f3fd91f5c4915f2021-11-25T16:14:39ZNew Alternative Mixtures of Cryoprotectants for Equine Immature Oocyte Vitrification10.3390/ani111130772076-2615https://doaj.org/article/d1a2155f14ce437fa5f3fd91f5c4915f2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3077https://doaj.org/toc/2076-2615Equine oocyte vitrification would benefit the growing in vitro embryo production programs, but further optimization of the protocol is necessary to reach clinical efficiency. Therefore, we aimed to perform a direct comparison of non-permeating and permeating cryoprotective agents (CPAs) during the vitrification and warming of equine immature oocytes. In the first experiment, cumulus oocytes complexes (COCs) were vitrified comparing sucrose, trehalose, and galactose in combination with ethylene glycol (EG) and dimethyl sulfoxide (DMSO). In the second experiment, the COCs were vitrified using three mixtures of permeating CPAs in a 50:50 volume ratio (ethylene glycol-dimethyl sulfoxide (ED), propylene glycol-ethylene glycol (PE), and propylene glycol-dimethyl sulfoxide (PD)) with galactose and warmed in different galactose concentrations (0.3 or 0.5 mol/L). Overall, all the treatments supported blastocyst formation, but the developmental rates were lower for all the vitrified groups in the first (4.3 to 7.6%) and the second (3.5 to 9.4%) experiment compared to the control (26.5 and 34.2%, respectively; <i>p</i> < 0.01). In the first experiment, the maturation was not affected by vitrification. The sucrose exhibited lower cleavage than the control (<i>p</i> = 0.02). Although the galactose tended to have lower maturation than trehalose (<i>p</i> = 0.060) and control (<i>p</i> = 0.069), the highest numerical cleavage and blastocyst rates were obtained with this CPA. In the second experiment, the maturation, cleavage, and blastocyst rates were similar between the treatments. Compared to the control, only the ED reached similar maturation (<i>p</i> = 0.02) and PE similar cleavage (<i>p</i> = 0.1). The galactose concentration during warming did not affect the maturation, cleavage, or blastocyst rates (<i>p</i> > 0.1), but the PE-0.3 exhibited the highest blastocyst rate (15.1%) among the treatments, being the only one comparable to the control (34.2%). As such, PE–galactose provides a valuable option for equine immature oocyte vitrification and should be considered for the future optimization of the protocol.Daniel Angel-VelezTine De CosterNima Azari-DolatabadAndrea Fernandez-MontoroCamilla BenedettiOsvaldo Bogado PascottiniHenri WoeldersAnn Van SoomKatrien SmitsMDPI AGarticlecryoprotective agentsequineICSIoocytevitrificationwarmingVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3077, p 3077 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cryoprotective agents equine ICSI oocyte vitrification warming Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	cryoprotective agents equine ICSI oocyte vitrification warming Veterinary medicine SF600-1100 Zoology QL1-991 Daniel Angel-Velez Tine De Coster Nima Azari-Dolatabad Andrea Fernandez-Montoro Camilla Benedetti Osvaldo Bogado Pascottini Henri Woelders Ann Van Soom Katrien Smits New Alternative Mixtures of Cryoprotectants for Equine Immature Oocyte Vitrification
description	Equine oocyte vitrification would benefit the growing in vitro embryo production programs, but further optimization of the protocol is necessary to reach clinical efficiency. Therefore, we aimed to perform a direct comparison of non-permeating and permeating cryoprotective agents (CPAs) during the vitrification and warming of equine immature oocytes. In the first experiment, cumulus oocytes complexes (COCs) were vitrified comparing sucrose, trehalose, and galactose in combination with ethylene glycol (EG) and dimethyl sulfoxide (DMSO). In the second experiment, the COCs were vitrified using three mixtures of permeating CPAs in a 50:50 volume ratio (ethylene glycol-dimethyl sulfoxide (ED), propylene glycol-ethylene glycol (PE), and propylene glycol-dimethyl sulfoxide (PD)) with galactose and warmed in different galactose concentrations (0.3 or 0.5 mol/L). Overall, all the treatments supported blastocyst formation, but the developmental rates were lower for all the vitrified groups in the first (4.3 to 7.6%) and the second (3.5 to 9.4%) experiment compared to the control (26.5 and 34.2%, respectively; <i>p</i> < 0.01). In the first experiment, the maturation was not affected by vitrification. The sucrose exhibited lower cleavage than the control (<i>p</i> = 0.02). Although the galactose tended to have lower maturation than trehalose (<i>p</i> = 0.060) and control (<i>p</i> = 0.069), the highest numerical cleavage and blastocyst rates were obtained with this CPA. In the second experiment, the maturation, cleavage, and blastocyst rates were similar between the treatments. Compared to the control, only the ED reached similar maturation (<i>p</i> = 0.02) and PE similar cleavage (<i>p</i> = 0.1). The galactose concentration during warming did not affect the maturation, cleavage, or blastocyst rates (<i>p</i> > 0.1), but the PE-0.3 exhibited the highest blastocyst rate (15.1%) among the treatments, being the only one comparable to the control (34.2%). As such, PE–galactose provides a valuable option for equine immature oocyte vitrification and should be considered for the future optimization of the protocol.
format	article
author	Daniel Angel-Velez Tine De Coster Nima Azari-Dolatabad Andrea Fernandez-Montoro Camilla Benedetti Osvaldo Bogado Pascottini Henri Woelders Ann Van Soom Katrien Smits
author_facet	Daniel Angel-Velez Tine De Coster Nima Azari-Dolatabad Andrea Fernandez-Montoro Camilla Benedetti Osvaldo Bogado Pascottini Henri Woelders Ann Van Soom Katrien Smits
author_sort	Daniel Angel-Velez
title	New Alternative Mixtures of Cryoprotectants for Equine Immature Oocyte Vitrification
title_short	New Alternative Mixtures of Cryoprotectants for Equine Immature Oocyte Vitrification
title_full	New Alternative Mixtures of Cryoprotectants for Equine Immature Oocyte Vitrification
title_fullStr	New Alternative Mixtures of Cryoprotectants for Equine Immature Oocyte Vitrification
title_full_unstemmed	New Alternative Mixtures of Cryoprotectants for Equine Immature Oocyte Vitrification
title_sort	new alternative mixtures of cryoprotectants for equine immature oocyte vitrification
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d1a2155f14ce437fa5f3fd91f5c4915f
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New Alternative Mixtures of Cryoprotectants for Equine Immature Oocyte Vitrification

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