Nutritional, phytochemical, and in vitro anticancer potential of sugar apple (Annona squamosa) fruits

Abstract In plants, Fruits and their wastes are the main sources of bioactive compounds. Currently, Annona fruits have attracted the attention of people interested in health-promoting foods due to their phytochemical content that their activities were not studied before. This study aimed to explore...

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Autores principales:	Mohamed Gamal Shehata, Marwa Muhammad Abu-Serie, Nourhan Mohammad Abd El-Aziz, Sobhy Ahmed El-Sohaimy
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d1a3428f26e448048a9a08643cf34a72
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spelling	oai:doaj.org-article:d1a3428f26e448048a9a08643cf34a722021-12-02T11:39:20ZNutritional, phytochemical, and in vitro anticancer potential of sugar apple (Annona squamosa) fruits10.1038/s41598-021-85772-82045-2322https://doaj.org/article/d1a3428f26e448048a9a08643cf34a722021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85772-8https://doaj.org/toc/2045-2322Abstract In plants, Fruits and their wastes are the main sources of bioactive compounds. Currently, Annona fruits have attracted the attention of people interested in health-promoting foods due to their phytochemical content that their activities were not studied before. This study aimed to explore the potential antioxidant, antimicrobial, and in vitro anticancer activity of two cultivars Annona squamosa (Annona b. and Annona h.) seed, peel, and pulp. We also meausred phenolic, flavonoid, sulfated polysaccharide, tannins, and triterpenoids. Polyphenol identification was determined using RP-HPLC. Results of the antioxidant activity revealed that the highest activity was observed for Annona h. seed extract using DPPH and ABTS assays with IC50 6.07 ± 0.50 and 9.58 ± 0.53 µg/ml, respectively. The antimicrobial activity against various pathogenic strains revealed that the peel extracts of both Annona b. and Annona h. exhibited the best antimicrobial activity. We also assessed the IC50 values for anticancer activity in all six Annona b. and Annona h samples against four cancer cell lines colon (Caco-2), prostate (PC3), liver (HepG-2), and breast (MCF-7) using MTT assay. Annona b. and Annona h seed extracts had the lowest IC50 values for four cancer cell lines with 7.31 ± 0.03 and 15.99 ± 1.25 for PC-3 and MCF-7, respectively. Both seed extracts, Annona b. and Annona h., showed significantly down-regulated mRNA expression of Bcl-2 and up-regulated p53 in all treated cell lines. Apoptosis was evaluated using nuclear staining, flow cytometric analysis, and immunohistochemistry of the proliferation marker (Ki-67). Additional studies are required to characterize the bioactive compounds responsible for the observed activities of Annona seed and determine its mechanism as an anticancer drug.Mohamed Gamal ShehataMarwa Muhammad Abu-SerieNourhan Mohammad Abd El-AzizSobhy Ahmed El-SohaimyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mohamed Gamal Shehata Marwa Muhammad Abu-Serie Nourhan Mohammad Abd El-Aziz Sobhy Ahmed El-Sohaimy Nutritional, phytochemical, and in vitro anticancer potential of sugar apple (Annona squamosa) fruits
description	Abstract In plants, Fruits and their wastes are the main sources of bioactive compounds. Currently, Annona fruits have attracted the attention of people interested in health-promoting foods due to their phytochemical content that their activities were not studied before. This study aimed to explore the potential antioxidant, antimicrobial, and in vitro anticancer activity of two cultivars Annona squamosa (Annona b. and Annona h.) seed, peel, and pulp. We also meausred phenolic, flavonoid, sulfated polysaccharide, tannins, and triterpenoids. Polyphenol identification was determined using RP-HPLC. Results of the antioxidant activity revealed that the highest activity was observed for Annona h. seed extract using DPPH and ABTS assays with IC50 6.07 ± 0.50 and 9.58 ± 0.53 µg/ml, respectively. The antimicrobial activity against various pathogenic strains revealed that the peel extracts of both Annona b. and Annona h. exhibited the best antimicrobial activity. We also assessed the IC50 values for anticancer activity in all six Annona b. and Annona h samples against four cancer cell lines colon (Caco-2), prostate (PC3), liver (HepG-2), and breast (MCF-7) using MTT assay. Annona b. and Annona h seed extracts had the lowest IC50 values for four cancer cell lines with 7.31 ± 0.03 and 15.99 ± 1.25 for PC-3 and MCF-7, respectively. Both seed extracts, Annona b. and Annona h., showed significantly down-regulated mRNA expression of Bcl-2 and up-regulated p53 in all treated cell lines. Apoptosis was evaluated using nuclear staining, flow cytometric analysis, and immunohistochemistry of the proliferation marker (Ki-67). Additional studies are required to characterize the bioactive compounds responsible for the observed activities of Annona seed and determine its mechanism as an anticancer drug.
format	article
author	Mohamed Gamal Shehata Marwa Muhammad Abu-Serie Nourhan Mohammad Abd El-Aziz Sobhy Ahmed El-Sohaimy
author_facet	Mohamed Gamal Shehata Marwa Muhammad Abu-Serie Nourhan Mohammad Abd El-Aziz Sobhy Ahmed El-Sohaimy
author_sort	Mohamed Gamal Shehata
title	Nutritional, phytochemical, and in vitro anticancer potential of sugar apple (Annona squamosa) fruits
title_short	Nutritional, phytochemical, and in vitro anticancer potential of sugar apple (Annona squamosa) fruits
title_full	Nutritional, phytochemical, and in vitro anticancer potential of sugar apple (Annona squamosa) fruits
title_fullStr	Nutritional, phytochemical, and in vitro anticancer potential of sugar apple (Annona squamosa) fruits
title_full_unstemmed	Nutritional, phytochemical, and in vitro anticancer potential of sugar apple (Annona squamosa) fruits
title_sort	nutritional, phytochemical, and in vitro anticancer potential of sugar apple (annona squamosa) fruits
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d1a3428f26e448048a9a08643cf34a72
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Nutritional, phytochemical, and in vitro anticancer potential of sugar apple (Annona squamosa) fruits

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