Specific matrix metalloproteinases play different roles in intraplaque angiogenesis and plaque instability in rabbits.

<h4>Background</h4>Ectopic angiogenesis within the intima and media is considered to be a hallmark of advanced vulnerable atherosclerotic lesions. Some studies have shown that specific matrix metalloproteinases (MMPs) might play different roles in angiogenesis. Therefore, we investigated...

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Autores principales:	Xiao Qiong Liu, Yang Mao, Bo Wang, Xiao Ting Lu, Wen Wu Bai, Yuan Yuan Sun, Yan Liu, Hong Mei Liu, Lei Zhang, Yu Xia Zhao, Yun Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d1a8119de0dc40b1a979670b58af87fd
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spelling	oai:doaj.org-article:d1a8119de0dc40b1a979670b58af87fd2021-11-25T06:00:09ZSpecific matrix metalloproteinases play different roles in intraplaque angiogenesis and plaque instability in rabbits.1932-620310.1371/journal.pone.0107851https://doaj.org/article/d1a8119de0dc40b1a979670b58af87fd2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0107851https://doaj.org/toc/1932-6203<h4>Background</h4>Ectopic angiogenesis within the intima and media is considered to be a hallmark of advanced vulnerable atherosclerotic lesions. Some studies have shown that specific matrix metalloproteinases (MMPs) might play different roles in angiogenesis. Therefore, we investigated the predominant effects of specific MMPs in intraplaque angiogenesis and plaque instability in a rabbit model of atherosclerosis.<h4>Methods and results</h4>New Zealand rabbits underwent balloon injury of the abdominal artery and ingestion of a high-cholesterol (1%) diet to establish an atherosclerotic animal model. At weeks 4, 6, 8, 10, and 12 after balloon injury, five rabbits were euthanized and the abdominal aorta was harvested. Blood lipid analysis, intravascular ultrasound imaging, pathologic and immunohistochemical expression studies, and western blotting were performed. From weeks 4 to 12, the expression of MMP-1, -2, -3, and -9 and vascular endothelial growth factor A (VEGF-A) increased with atherosclerotic plaque development in the abdominal aorta, while the expression of MMP-14 substantially decreased. The vulnerability index (VI) gradually increased over time. Intraplaque neovessels appeared at week 8. The microvessel density (MVD) was greater at week 12 than at week 8. The VI, MVD, and VEGF-A level were positively correlated with the MMP-1, -2,-3, and -9 levels within plaques. Negative correlations were noted between the MMP-14 level and the VI, MVD, and VEGF-A level.<h4>Conclusion</h4>Upregulation of MMP-1, -2, -3, and -9 and downregulation of MMP-14 may contribute to intraplaque angiogenesis and plaque instability at the advanced stage of atherosclerosis in rabbits.Xiao Qiong LiuYang MaoBo WangXiao Ting LuWen Wu BaiYuan Yuan SunYan LiuHong Mei LiuLei ZhangYu Xia ZhaoYun ZhangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e107851 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiao Qiong Liu Yang Mao Bo Wang Xiao Ting Lu Wen Wu Bai Yuan Yuan Sun Yan Liu Hong Mei Liu Lei Zhang Yu Xia Zhao Yun Zhang Specific matrix metalloproteinases play different roles in intraplaque angiogenesis and plaque instability in rabbits.
description	<h4>Background</h4>Ectopic angiogenesis within the intima and media is considered to be a hallmark of advanced vulnerable atherosclerotic lesions. Some studies have shown that specific matrix metalloproteinases (MMPs) might play different roles in angiogenesis. Therefore, we investigated the predominant effects of specific MMPs in intraplaque angiogenesis and plaque instability in a rabbit model of atherosclerosis.<h4>Methods and results</h4>New Zealand rabbits underwent balloon injury of the abdominal artery and ingestion of a high-cholesterol (1%) diet to establish an atherosclerotic animal model. At weeks 4, 6, 8, 10, and 12 after balloon injury, five rabbits were euthanized and the abdominal aorta was harvested. Blood lipid analysis, intravascular ultrasound imaging, pathologic and immunohistochemical expression studies, and western blotting were performed. From weeks 4 to 12, the expression of MMP-1, -2, -3, and -9 and vascular endothelial growth factor A (VEGF-A) increased with atherosclerotic plaque development in the abdominal aorta, while the expression of MMP-14 substantially decreased. The vulnerability index (VI) gradually increased over time. Intraplaque neovessels appeared at week 8. The microvessel density (MVD) was greater at week 12 than at week 8. The VI, MVD, and VEGF-A level were positively correlated with the MMP-1, -2,-3, and -9 levels within plaques. Negative correlations were noted between the MMP-14 level and the VI, MVD, and VEGF-A level.<h4>Conclusion</h4>Upregulation of MMP-1, -2, -3, and -9 and downregulation of MMP-14 may contribute to intraplaque angiogenesis and plaque instability at the advanced stage of atherosclerosis in rabbits.
format	article
author	Xiao Qiong Liu Yang Mao Bo Wang Xiao Ting Lu Wen Wu Bai Yuan Yuan Sun Yan Liu Hong Mei Liu Lei Zhang Yu Xia Zhao Yun Zhang
author_facet	Xiao Qiong Liu Yang Mao Bo Wang Xiao Ting Lu Wen Wu Bai Yuan Yuan Sun Yan Liu Hong Mei Liu Lei Zhang Yu Xia Zhao Yun Zhang
author_sort	Xiao Qiong Liu
title	Specific matrix metalloproteinases play different roles in intraplaque angiogenesis and plaque instability in rabbits.
title_short	Specific matrix metalloproteinases play different roles in intraplaque angiogenesis and plaque instability in rabbits.
title_full	Specific matrix metalloproteinases play different roles in intraplaque angiogenesis and plaque instability in rabbits.
title_fullStr	Specific matrix metalloproteinases play different roles in intraplaque angiogenesis and plaque instability in rabbits.
title_full_unstemmed	Specific matrix metalloproteinases play different roles in intraplaque angiogenesis and plaque instability in rabbits.
title_sort	specific matrix metalloproteinases play different roles in intraplaque angiogenesis and plaque instability in rabbits.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/d1a8119de0dc40b1a979670b58af87fd
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Specific matrix metalloproteinases play different roles in intraplaque angiogenesis and plaque instability in rabbits.

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