Relationship between the Biofilm-Forming Capacity and Antimicrobial Resistance in Clinical <i>Acinetobacter baumannii</i> Isolates: Results from a Laboratory-Based In Vitro Study

The relationship between the multidrug-resistant (MDR) phenotype and biofilm-forming capacity has been a topic of extensive interest among biomedical scientists, as these two factors may have significant influence on the outcomes of infections. The aim of the present study was to establish a possibl...

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Autores principales:	Matthew Gavino Donadu, Vittorio Mazzarello, Piero Cappuccinelli, Stefania Zanetti, Melinda Madléna, Ádám László Nagy, Anette Stájer, Katalin Burián, Márió Gajdács
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Acinetobacter baumannii</i> antibiotic resistance multidrug resistance MDR biofilm crystal violet Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d1a9c9c7198044809d2360aa38a27e9b
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spelling	oai:doaj.org-article:d1a9c9c7198044809d2360aa38a27e9b2021-11-25T18:25:35ZRelationship between the Biofilm-Forming Capacity and Antimicrobial Resistance in Clinical <i>Acinetobacter baumannii</i> Isolates: Results from a Laboratory-Based In Vitro Study10.3390/microorganisms91123842076-2607https://doaj.org/article/d1a9c9c7198044809d2360aa38a27e9b2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2384https://doaj.org/toc/2076-2607The relationship between the multidrug-resistant (MDR) phenotype and biofilm-forming capacity has been a topic of extensive interest among biomedical scientists, as these two factors may have significant influence on the outcomes of infections. The aim of the present study was to establish a possible relationship between biofilm-forming capacity and the antibiotic-resistant phenotype in clinical <i>Acinetobacter baumannii</i> (<i>A. baumannii</i>) isolates. A total of n = 309 isolates were included in this study. Antimicrobial susceptibility testing and the phenotypic detection of resistance determinants were carried out. The capacity of isolates to produce biofilms was assessed using a crystal violet microtiter-plate-based method. Resistance rates were highest for ciprofloxacin (71.19%; n = 220), levofloxacin (n = 68.61%; n = 212), and trimethoprim-sulfamethoxazole (n = 66.02%; n = 209); 42.72% (n = 132) of isolates were classified as MDR; 22.65% (n = 70) of tested isolates were positive in the modified Hodge-test; the overexpression of efflux pumps had significant effects on the susceptibilities of meropenem, gentamicin, and ciprofloxacin in 14.24% (n = 44), 6.05% (n = 19), and 27.51% (n = 85), respectively; 9.39% (n = 29), 12.29% (n = 38), 22.97% (n = 71), and 55.35% (n = 170) of isolates were non-biofilm-producing and weak, moderate, and strong biofilm producers, respectively. A numerical, but statistically not significant, difference was identified between the MDR and non-MDR isolates regarding their biofilm-forming capacity (MDR: 0.495 ± 0.309 vs. non-MDR: 0.545 ± 0.283; <i>p</i> = 0.072), and no association was seen between resistance to individual antibiotics and biofilm formation. Based on numerical trends, MER-resistant isolates were the strongest biofilm producers (<i>p</i> = 0.067). Our study emphasizes the need for additional experiments to assess the role biofilms have in the pathogenesis of <i>A. baumannii</i> infections.Matthew Gavino DonaduVittorio MazzarelloPiero CappuccinelliStefania ZanettiMelinda MadlénaÁdám László NagyAnette StájerKatalin BuriánMárió GajdácsMDPI AGarticle<i>Acinetobacter baumannii</i>antibiotic resistancemultidrug resistanceMDRbiofilmcrystal violetBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2384, p 2384 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Acinetobacter baumannii</i> antibiotic resistance multidrug resistance MDR biofilm crystal violet Biology (General) QH301-705.5
spellingShingle	<i>Acinetobacter baumannii</i> antibiotic resistance multidrug resistance MDR biofilm crystal violet Biology (General) QH301-705.5 Matthew Gavino Donadu Vittorio Mazzarello Piero Cappuccinelli Stefania Zanetti Melinda Madléna Ádám László Nagy Anette Stájer Katalin Burián Márió Gajdács Relationship between the Biofilm-Forming Capacity and Antimicrobial Resistance in Clinical <i>Acinetobacter baumannii</i> Isolates: Results from a Laboratory-Based In Vitro Study
description	The relationship between the multidrug-resistant (MDR) phenotype and biofilm-forming capacity has been a topic of extensive interest among biomedical scientists, as these two factors may have significant influence on the outcomes of infections. The aim of the present study was to establish a possible relationship between biofilm-forming capacity and the antibiotic-resistant phenotype in clinical <i>Acinetobacter baumannii</i> (<i>A. baumannii</i>) isolates. A total of n = 309 isolates were included in this study. Antimicrobial susceptibility testing and the phenotypic detection of resistance determinants were carried out. The capacity of isolates to produce biofilms was assessed using a crystal violet microtiter-plate-based method. Resistance rates were highest for ciprofloxacin (71.19%; n = 220), levofloxacin (n = 68.61%; n = 212), and trimethoprim-sulfamethoxazole (n = 66.02%; n = 209); 42.72% (n = 132) of isolates were classified as MDR; 22.65% (n = 70) of tested isolates were positive in the modified Hodge-test; the overexpression of efflux pumps had significant effects on the susceptibilities of meropenem, gentamicin, and ciprofloxacin in 14.24% (n = 44), 6.05% (n = 19), and 27.51% (n = 85), respectively; 9.39% (n = 29), 12.29% (n = 38), 22.97% (n = 71), and 55.35% (n = 170) of isolates were non-biofilm-producing and weak, moderate, and strong biofilm producers, respectively. A numerical, but statistically not significant, difference was identified between the MDR and non-MDR isolates regarding their biofilm-forming capacity (MDR: 0.495 ± 0.309 vs. non-MDR: 0.545 ± 0.283; <i>p</i> = 0.072), and no association was seen between resistance to individual antibiotics and biofilm formation. Based on numerical trends, MER-resistant isolates were the strongest biofilm producers (<i>p</i> = 0.067). Our study emphasizes the need for additional experiments to assess the role biofilms have in the pathogenesis of <i>A. baumannii</i> infections.
format	article
author	Matthew Gavino Donadu Vittorio Mazzarello Piero Cappuccinelli Stefania Zanetti Melinda Madléna Ádám László Nagy Anette Stájer Katalin Burián Márió Gajdács
author_facet	Matthew Gavino Donadu Vittorio Mazzarello Piero Cappuccinelli Stefania Zanetti Melinda Madléna Ádám László Nagy Anette Stájer Katalin Burián Márió Gajdács
author_sort	Matthew Gavino Donadu
title	Relationship between the Biofilm-Forming Capacity and Antimicrobial Resistance in Clinical <i>Acinetobacter baumannii</i> Isolates: Results from a Laboratory-Based In Vitro Study
title_short	Relationship between the Biofilm-Forming Capacity and Antimicrobial Resistance in Clinical <i>Acinetobacter baumannii</i> Isolates: Results from a Laboratory-Based In Vitro Study
title_full	Relationship between the Biofilm-Forming Capacity and Antimicrobial Resistance in Clinical <i>Acinetobacter baumannii</i> Isolates: Results from a Laboratory-Based In Vitro Study
title_fullStr	Relationship between the Biofilm-Forming Capacity and Antimicrobial Resistance in Clinical <i>Acinetobacter baumannii</i> Isolates: Results from a Laboratory-Based In Vitro Study
title_full_unstemmed	Relationship between the Biofilm-Forming Capacity and Antimicrobial Resistance in Clinical <i>Acinetobacter baumannii</i> Isolates: Results from a Laboratory-Based In Vitro Study
title_sort	relationship between the biofilm-forming capacity and antimicrobial resistance in clinical <i>acinetobacter baumannii</i> isolates: results from a laboratory-based in vitro study
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d1a9c9c7198044809d2360aa38a27e9b
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Relationship between the Biofilm-Forming Capacity and Antimicrobial Resistance in Clinical <i>Acinetobacter baumannii</i> Isolates: Results from a Laboratory-Based In Vitro Study

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