Milk exosomes are bioavailable and distinct microRNA cargos have unique tissue distribution patterns

Abstract Exosomes participate in cell-to-cell communication, facilitated by the transfer of RNAs, proteins and lipids from donor to recipient cells. Exosomes and their RNA cargos do not exclusively originate from endogenous synthesis but may also be obtained from dietary sources such as the inter-sp...

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Autores principales:	Sonia Manca, Bijaya Upadhyaya, Ezra Mutai, Amy T. Desaulniers, Rebecca A. Cederberg, Brett R. White, Janos Zempleni
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d1aac8aef5bf4e3f9c8e1dd99276ab17
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spelling	oai:doaj.org-article:d1aac8aef5bf4e3f9c8e1dd99276ab172021-12-02T15:08:05ZMilk exosomes are bioavailable and distinct microRNA cargos have unique tissue distribution patterns10.1038/s41598-018-29780-12045-2322https://doaj.org/article/d1aac8aef5bf4e3f9c8e1dd99276ab172018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-29780-1https://doaj.org/toc/2045-2322Abstract Exosomes participate in cell-to-cell communication, facilitated by the transfer of RNAs, proteins and lipids from donor to recipient cells. Exosomes and their RNA cargos do not exclusively originate from endogenous synthesis but may also be obtained from dietary sources such as the inter-species transfer of exosomes and RNAs in bovine milk to humans. Here, we assessed the bioavailability and distribution of exosomes and their microRNA cargos from bovine, porcine and murine milk within and across species boundaries. Milk exosomes labeled with fluorophores or fluorescent fusion proteins accumulated in liver, spleen and brain following suckling, oral gavage and intravenous administration in mice and pigs. When synthetic, fluorophore-labeled microRNAs were transfected into bovine milk exosomes and administered to mice, distinct species of microRNAs demonstrated unique distribution profiles and accumulated in intestinal mucosa, spleen, liver, heart or brain. Administration of bovine milk exosomes failed to rescue Drosha homozygous knockout mice, presumably due to low bioavailability or lack of essential microRNAs.Sonia MancaBijaya UpadhyayaEzra MutaiAmy T. DesaulniersRebecca A. CederbergBrett R. WhiteJanos ZempleniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sonia Manca Bijaya Upadhyaya Ezra Mutai Amy T. Desaulniers Rebecca A. Cederberg Brett R. White Janos Zempleni Milk exosomes are bioavailable and distinct microRNA cargos have unique tissue distribution patterns
description	Abstract Exosomes participate in cell-to-cell communication, facilitated by the transfer of RNAs, proteins and lipids from donor to recipient cells. Exosomes and their RNA cargos do not exclusively originate from endogenous synthesis but may also be obtained from dietary sources such as the inter-species transfer of exosomes and RNAs in bovine milk to humans. Here, we assessed the bioavailability and distribution of exosomes and their microRNA cargos from bovine, porcine and murine milk within and across species boundaries. Milk exosomes labeled with fluorophores or fluorescent fusion proteins accumulated in liver, spleen and brain following suckling, oral gavage and intravenous administration in mice and pigs. When synthetic, fluorophore-labeled microRNAs were transfected into bovine milk exosomes and administered to mice, distinct species of microRNAs demonstrated unique distribution profiles and accumulated in intestinal mucosa, spleen, liver, heart or brain. Administration of bovine milk exosomes failed to rescue Drosha homozygous knockout mice, presumably due to low bioavailability or lack of essential microRNAs.
format	article
author	Sonia Manca Bijaya Upadhyaya Ezra Mutai Amy T. Desaulniers Rebecca A. Cederberg Brett R. White Janos Zempleni
author_facet	Sonia Manca Bijaya Upadhyaya Ezra Mutai Amy T. Desaulniers Rebecca A. Cederberg Brett R. White Janos Zempleni
author_sort	Sonia Manca
title	Milk exosomes are bioavailable and distinct microRNA cargos have unique tissue distribution patterns
title_short	Milk exosomes are bioavailable and distinct microRNA cargos have unique tissue distribution patterns
title_full	Milk exosomes are bioavailable and distinct microRNA cargos have unique tissue distribution patterns
title_fullStr	Milk exosomes are bioavailable and distinct microRNA cargos have unique tissue distribution patterns
title_full_unstemmed	Milk exosomes are bioavailable and distinct microRNA cargos have unique tissue distribution patterns
title_sort	milk exosomes are bioavailable and distinct microrna cargos have unique tissue distribution patterns
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/d1aac8aef5bf4e3f9c8e1dd99276ab17
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Milk exosomes are bioavailable and distinct microRNA cargos have unique tissue distribution patterns

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