Profiling of long non-coding RNAs identifies LINC00958 and LINC01296 as candidate oncogenes in bladder cancer
Abstract Aberrant expression of long non-coding RNAs (lncRNAs) has been regarded as a critical component in bladder cancer (BC) and lncRNAs have been associated with BC development and progression although their overall expression and functional significance is still unclear. The aim of our study wa...
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Nature Portfolio
2017
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oai:doaj.org-article:d1bf7670985049f9b07d1d977c9124842021-12-02T15:06:08ZProfiling of long non-coding RNAs identifies LINC00958 and LINC01296 as candidate oncogenes in bladder cancer10.1038/s41598-017-00327-02045-2322https://doaj.org/article/d1bf7670985049f9b07d1d977c9124842017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00327-0https://doaj.org/toc/2045-2322Abstract Aberrant expression of long non-coding RNAs (lncRNAs) has been regarded as a critical component in bladder cancer (BC) and lncRNAs have been associated with BC development and progression although their overall expression and functional significance is still unclear. The aim of our study was to identify novel lncRNAs with a functional role in BC carcinogenesis. RNA-sequencing was used to identify aberrantly expressed lncRNAs in 8 normal and 72 BC samples. We identified 89 lncRNAs that were significantly dys-regulated in BC. Five lncRNAs; LINC00958, LINC01296, LINC00355, LNC-CMC1-1 and LNC-ALX1-2 were selected for further analyses. Silencing of LINC00958 or LINC01296 in vitro reduced both cell viability and migration. Knock-down of LINC00958 also affected invasion and resistance to anoikis. These cellular effects could be linked to direct/indirect regulation of protein coding mRNAs involved in cell death/survival, proliferation and cellular movement. Finally, we showed that LINC00958 binds proteins involved in regulation and initiation of translation and in post-transcriptional modification of RNA, including Metadherin, which has previously been associated with BC. Our analyses identified novel lncRNAs in BC that likely act as oncogenic drivers contributing to an aggressive cancerous phenotype likely through interaction with proteins involved in initiation of translation and/or post-transcriptional modification of RNA.Anna Katharina SeitzLise Lotte ChristensenEmil ChristensenKasper FaarkrogMarie Stampe OstenfeldJakob HedegaardIver NordentoftMorten Muhlig NielsenJohan PalmfeldtMichelle ThomsonMichael Theis Solgaard JensenRoman NawrothTobias MaurerTorben Falck ØrntoftJørgen Bjerggaard JensenChristian Kroun DamgaardLars DyrskjøtNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017) |
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Medicine R Science Q Anna Katharina Seitz Lise Lotte Christensen Emil Christensen Kasper Faarkrog Marie Stampe Ostenfeld Jakob Hedegaard Iver Nordentoft Morten Muhlig Nielsen Johan Palmfeldt Michelle Thomson Michael Theis Solgaard Jensen Roman Nawroth Tobias Maurer Torben Falck Ørntoft Jørgen Bjerggaard Jensen Christian Kroun Damgaard Lars Dyrskjøt Profiling of long non-coding RNAs identifies LINC00958 and LINC01296 as candidate oncogenes in bladder cancer |
| description |
Abstract Aberrant expression of long non-coding RNAs (lncRNAs) has been regarded as a critical component in bladder cancer (BC) and lncRNAs have been associated with BC development and progression although their overall expression and functional significance is still unclear. The aim of our study was to identify novel lncRNAs with a functional role in BC carcinogenesis. RNA-sequencing was used to identify aberrantly expressed lncRNAs in 8 normal and 72 BC samples. We identified 89 lncRNAs that were significantly dys-regulated in BC. Five lncRNAs; LINC00958, LINC01296, LINC00355, LNC-CMC1-1 and LNC-ALX1-2 were selected for further analyses. Silencing of LINC00958 or LINC01296 in vitro reduced both cell viability and migration. Knock-down of LINC00958 also affected invasion and resistance to anoikis. These cellular effects could be linked to direct/indirect regulation of protein coding mRNAs involved in cell death/survival, proliferation and cellular movement. Finally, we showed that LINC00958 binds proteins involved in regulation and initiation of translation and in post-transcriptional modification of RNA, including Metadherin, which has previously been associated with BC. Our analyses identified novel lncRNAs in BC that likely act as oncogenic drivers contributing to an aggressive cancerous phenotype likely through interaction with proteins involved in initiation of translation and/or post-transcriptional modification of RNA. |
| format |
article |
| author |
Anna Katharina Seitz Lise Lotte Christensen Emil Christensen Kasper Faarkrog Marie Stampe Ostenfeld Jakob Hedegaard Iver Nordentoft Morten Muhlig Nielsen Johan Palmfeldt Michelle Thomson Michael Theis Solgaard Jensen Roman Nawroth Tobias Maurer Torben Falck Ørntoft Jørgen Bjerggaard Jensen Christian Kroun Damgaard Lars Dyrskjøt |
| author_facet |
Anna Katharina Seitz Lise Lotte Christensen Emil Christensen Kasper Faarkrog Marie Stampe Ostenfeld Jakob Hedegaard Iver Nordentoft Morten Muhlig Nielsen Johan Palmfeldt Michelle Thomson Michael Theis Solgaard Jensen Roman Nawroth Tobias Maurer Torben Falck Ørntoft Jørgen Bjerggaard Jensen Christian Kroun Damgaard Lars Dyrskjøt |
| author_sort |
Anna Katharina Seitz |
| title |
Profiling of long non-coding RNAs identifies LINC00958 and LINC01296 as candidate oncogenes in bladder cancer |
| title_short |
Profiling of long non-coding RNAs identifies LINC00958 and LINC01296 as candidate oncogenes in bladder cancer |
| title_full |
Profiling of long non-coding RNAs identifies LINC00958 and LINC01296 as candidate oncogenes in bladder cancer |
| title_fullStr |
Profiling of long non-coding RNAs identifies LINC00958 and LINC01296 as candidate oncogenes in bladder cancer |
| title_full_unstemmed |
Profiling of long non-coding RNAs identifies LINC00958 and LINC01296 as candidate oncogenes in bladder cancer |
| title_sort |
profiling of long non-coding rnas identifies linc00958 and linc01296 as candidate oncogenes in bladder cancer |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/d1bf7670985049f9b07d1d977c912484 |
| work_keys_str_mv |
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| _version_ |
1718388612243914752 |