MONOCLONAL ANTIBODIES AND RECOMBINANT PROTEINS OF FILOVIRUSES: IMMUNOCHEMICAL PROPERTIES AND EVALUATION OF THEIR EFFICIENCY FOR IMMUNE DIAGNOSTICS OF MARBURG AND EBOLA VIRUSES

A panel of monoclonal antibodies (MAbs) against VP35, VP40 and NP viral proteins of Marburg and Ebola viruses, as well as recombinant VP35, VP40 and NP proteins were generated and tested for their capacity to specific immune reactions. Monoclonal antibodies to appropriate viral proteins effectively...

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Autores principales:	E. I. Kazachinskaia, A. V. Ivanova, A. V. Sorokin, E. L. Subbotina, A. V. Kachko, I. A. Razumov, V. B. Loktev
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	marburg virus (mv) ebola virus (ev) monoclonal antibodies (mabs) recombinant proteins immune diagnostics Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/d1c0a2f282e34974b71a4c413c0118cf
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spelling	oai:doaj.org-article:d1c0a2f282e34974b71a4c413c0118cf2021-11-18T08:03:37ZMONOCLONAL ANTIBODIES AND RECOMBINANT PROTEINS OF FILOVIRUSES: IMMUNOCHEMICAL PROPERTIES AND EVALUATION OF THEIR EFFICIENCY FOR IMMUNE DIAGNOSTICS OF MARBURG AND EBOLA VIRUSES1563-06252313-741X10.15789/1563-0625-2010-3-177-190https://doaj.org/article/d1c0a2f282e34974b71a4c413c0118cf2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/71https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XA panel of monoclonal antibodies (MAbs) against VP35, VP40 and NP viral proteins of Marburg and Ebola viruses, as well as recombinant VP35, VP40 and NP proteins were generated and tested for their capacity to specific immune reactions. Monoclonal antibodies to appropriate viral proteins effectively recognized the VP35, VP40 and NP recombinant proteins, thus allowing to develop a variant of a MAb-based ELISA analysis with different types of biotin-labeled MAbs, using these antibodies for capturing viral and recombinant antigens of Marburg and Ebola viruses. These techniques were able to detect viral and recombinant proteins in a concentration range between 1 and 150 ng/ml. We conclude that the recombinant VP35, VP40 and NP proteins of filoviruses, as well as MAbs against these viral proteins represent a promising tool for a new generation of immunodiagnostic kits and studying immunological features of filovirus infection.E. I. KazachinskaiaA. V. IvanovaA. V. SorokinE. L. SubbotinaA. V. KachkoI. A. RazumovV. B. LoktevSPb RAACIarticlemarburg virus (mv)ebola virus (ev)monoclonal antibodies (mabs)recombinant proteinsimmune diagnosticsImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 12, Iss 3, Pp 177-190 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	marburg virus (mv) ebola virus (ev) monoclonal antibodies (mabs) recombinant proteins immune diagnostics Immunologic diseases. Allergy RC581-607
spellingShingle	marburg virus (mv) ebola virus (ev) monoclonal antibodies (mabs) recombinant proteins immune diagnostics Immunologic diseases. Allergy RC581-607 E. I. Kazachinskaia A. V. Ivanova A. V. Sorokin E. L. Subbotina A. V. Kachko I. A. Razumov V. B. Loktev MONOCLONAL ANTIBODIES AND RECOMBINANT PROTEINS OF FILOVIRUSES: IMMUNOCHEMICAL PROPERTIES AND EVALUATION OF THEIR EFFICIENCY FOR IMMUNE DIAGNOSTICS OF MARBURG AND EBOLA VIRUSES
description	A panel of monoclonal antibodies (MAbs) against VP35, VP40 and NP viral proteins of Marburg and Ebola viruses, as well as recombinant VP35, VP40 and NP proteins were generated and tested for their capacity to specific immune reactions. Monoclonal antibodies to appropriate viral proteins effectively recognized the VP35, VP40 and NP recombinant proteins, thus allowing to develop a variant of a MAb-based ELISA analysis with different types of biotin-labeled MAbs, using these antibodies for capturing viral and recombinant antigens of Marburg and Ebola viruses. These techniques were able to detect viral and recombinant proteins in a concentration range between 1 and 150 ng/ml. We conclude that the recombinant VP35, VP40 and NP proteins of filoviruses, as well as MAbs against these viral proteins represent a promising tool for a new generation of immunodiagnostic kits and studying immunological features of filovirus infection.
format	article
author	E. I. Kazachinskaia A. V. Ivanova A. V. Sorokin E. L. Subbotina A. V. Kachko I. A. Razumov V. B. Loktev
author_facet	E. I. Kazachinskaia A. V. Ivanova A. V. Sorokin E. L. Subbotina A. V. Kachko I. A. Razumov V. B. Loktev
author_sort	E. I. Kazachinskaia
title	MONOCLONAL ANTIBODIES AND RECOMBINANT PROTEINS OF FILOVIRUSES: IMMUNOCHEMICAL PROPERTIES AND EVALUATION OF THEIR EFFICIENCY FOR IMMUNE DIAGNOSTICS OF MARBURG AND EBOLA VIRUSES
title_short	MONOCLONAL ANTIBODIES AND RECOMBINANT PROTEINS OF FILOVIRUSES: IMMUNOCHEMICAL PROPERTIES AND EVALUATION OF THEIR EFFICIENCY FOR IMMUNE DIAGNOSTICS OF MARBURG AND EBOLA VIRUSES
title_full	MONOCLONAL ANTIBODIES AND RECOMBINANT PROTEINS OF FILOVIRUSES: IMMUNOCHEMICAL PROPERTIES AND EVALUATION OF THEIR EFFICIENCY FOR IMMUNE DIAGNOSTICS OF MARBURG AND EBOLA VIRUSES
title_fullStr	MONOCLONAL ANTIBODIES AND RECOMBINANT PROTEINS OF FILOVIRUSES: IMMUNOCHEMICAL PROPERTIES AND EVALUATION OF THEIR EFFICIENCY FOR IMMUNE DIAGNOSTICS OF MARBURG AND EBOLA VIRUSES
title_full_unstemmed	MONOCLONAL ANTIBODIES AND RECOMBINANT PROTEINS OF FILOVIRUSES: IMMUNOCHEMICAL PROPERTIES AND EVALUATION OF THEIR EFFICIENCY FOR IMMUNE DIAGNOSTICS OF MARBURG AND EBOLA VIRUSES
title_sort	monoclonal antibodies and recombinant proteins of filoviruses: immunochemical properties and evaluation of their efficiency for immune diagnostics of marburg and ebola viruses
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/d1c0a2f282e34974b71a4c413c0118cf
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MONOCLONAL ANTIBODIES AND RECOMBINANT PROTEINS OF FILOVIRUSES: IMMUNOCHEMICAL PROPERTIES AND EVALUATION OF THEIR EFFICIENCY FOR IMMUNE DIAGNOSTICS OF MARBURG AND EBOLA VIRUSES

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