Therapeutic effect of bone marrow mesenchymal stem cells in a rat model of carbon tetrachloride induced liver fibrosisAt a glance of commentary

Background: Liver fibrosis is a major medical problem with high mortality and morbidity rates where the formation of regenerative nodules and cirrhosis leads to loss of liver function and may result in the development of hepatocellular carcinoma. bone marrow mesenchymal stem cells (BM-MSCs) have dra...

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Autores principales:	Mohammed R. Khalil, Reda S. El-Demerdash, Hazem H. Elminshawy, Eman T. Mehanna, Noha M. Mesbah, Dina M. Abo-Elmatty
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Bone marrow mesenchymal stem cells CYP450 Liver fibrosis Medicine (General) R5-920 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d1cecbb5d7eb4fa2bf8418886ff733cc
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spelling	oai:doaj.org-article:d1cecbb5d7eb4fa2bf8418886ff733cc2021-11-28T04:33:03ZTherapeutic effect of bone marrow mesenchymal stem cells in a rat model of carbon tetrachloride induced liver fibrosisAt a glance of commentary2319-417010.1016/j.bj.2020.04.011https://doaj.org/article/d1cecbb5d7eb4fa2bf8418886ff733cc2021-10-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2319417020300482https://doaj.org/toc/2319-4170Background: Liver fibrosis is a major medical problem with high mortality and morbidity rates where the formation of regenerative nodules and cirrhosis leads to loss of liver function and may result in the development of hepatocellular carcinoma. bone marrow mesenchymal stem cells (BM-MSCs) have drawn attention as a novel approach for treatment of liver fibrosis. This study aimed to evaluate the therapeutic effect of BM-MSCs on the liver structure in carbon tetrachloride (CCl4) induced liver fibrosis in male rats relative to resveratrol and Silybum marianum as standard drugs derived from herbal plants. Methods: Fifty adult male albino rats (Sprague Dawley strain; 180–220 g mean body weight) were purchased from the Laboratory Animal Unit in the Nile Center of Experimental Research, Mansoura, Egypt. Liver function were determined, isolation and preparation of BM- MSCs and detection of cell-surface markers by flow cytometry. Results: Animals exposed to CCl4 developed liver injury characterized by significant increase of liver enzymes, malondialdehyde (MDA), tumor necrosis factor alpha (TNFα), and CYP450, inhibition of antioxidant enzymes, and decreased albumin. Treatment with stem cells enhanced liver state more effectively than resveratrol and S. marianum. It significantly decreased AST, ALT, ALP, MDA, TNF-α, and CYP450 and increased albumin, SOD, GSH, GST, and CAT. Histopathological study and atomic force microscope results confirmed the therapeutic effects of MSCs. Conclusions: BM-MSCs could restore liver structure and function in CCL4 induced liver fibrosis rat model, ameliorating the toxicity of CCl4 and improving liver function tests.Mohammed R. KhalilReda S. El-DemerdashHazem H. ElminshawyEman T. MehannaNoha M. MesbahDina M. Abo-ElmattyElsevierarticleBone marrow mesenchymal stem cellsCYP450Liver fibrosisMedicine (General)R5-920Biology (General)QH301-705.5ENBiomedical Journal, Vol 44, Iss 5, Pp 598-610 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bone marrow mesenchymal stem cells CYP450 Liver fibrosis Medicine (General) R5-920 Biology (General) QH301-705.5
spellingShingle	Bone marrow mesenchymal stem cells CYP450 Liver fibrosis Medicine (General) R5-920 Biology (General) QH301-705.5 Mohammed R. Khalil Reda S. El-Demerdash Hazem H. Elminshawy Eman T. Mehanna Noha M. Mesbah Dina M. Abo-Elmatty Therapeutic effect of bone marrow mesenchymal stem cells in a rat model of carbon tetrachloride induced liver fibrosisAt a glance of commentary
description	Background: Liver fibrosis is a major medical problem with high mortality and morbidity rates where the formation of regenerative nodules and cirrhosis leads to loss of liver function and may result in the development of hepatocellular carcinoma. bone marrow mesenchymal stem cells (BM-MSCs) have drawn attention as a novel approach for treatment of liver fibrosis. This study aimed to evaluate the therapeutic effect of BM-MSCs on the liver structure in carbon tetrachloride (CCl4) induced liver fibrosis in male rats relative to resveratrol and Silybum marianum as standard drugs derived from herbal plants. Methods: Fifty adult male albino rats (Sprague Dawley strain; 180–220 g mean body weight) were purchased from the Laboratory Animal Unit in the Nile Center of Experimental Research, Mansoura, Egypt. Liver function were determined, isolation and preparation of BM- MSCs and detection of cell-surface markers by flow cytometry. Results: Animals exposed to CCl4 developed liver injury characterized by significant increase of liver enzymes, malondialdehyde (MDA), tumor necrosis factor alpha (TNFα), and CYP450, inhibition of antioxidant enzymes, and decreased albumin. Treatment with stem cells enhanced liver state more effectively than resveratrol and S. marianum. It significantly decreased AST, ALT, ALP, MDA, TNF-α, and CYP450 and increased albumin, SOD, GSH, GST, and CAT. Histopathological study and atomic force microscope results confirmed the therapeutic effects of MSCs. Conclusions: BM-MSCs could restore liver structure and function in CCL4 induced liver fibrosis rat model, ameliorating the toxicity of CCl4 and improving liver function tests.
format	article
author	Mohammed R. Khalil Reda S. El-Demerdash Hazem H. Elminshawy Eman T. Mehanna Noha M. Mesbah Dina M. Abo-Elmatty
author_facet	Mohammed R. Khalil Reda S. El-Demerdash Hazem H. Elminshawy Eman T. Mehanna Noha M. Mesbah Dina M. Abo-Elmatty
author_sort	Mohammed R. Khalil
title	Therapeutic effect of bone marrow mesenchymal stem cells in a rat model of carbon tetrachloride induced liver fibrosisAt a glance of commentary
title_short	Therapeutic effect of bone marrow mesenchymal stem cells in a rat model of carbon tetrachloride induced liver fibrosisAt a glance of commentary
title_full	Therapeutic effect of bone marrow mesenchymal stem cells in a rat model of carbon tetrachloride induced liver fibrosisAt a glance of commentary
title_fullStr	Therapeutic effect of bone marrow mesenchymal stem cells in a rat model of carbon tetrachloride induced liver fibrosisAt a glance of commentary
title_full_unstemmed	Therapeutic effect of bone marrow mesenchymal stem cells in a rat model of carbon tetrachloride induced liver fibrosisAt a glance of commentary
title_sort	therapeutic effect of bone marrow mesenchymal stem cells in a rat model of carbon tetrachloride induced liver fibrosisat a glance of commentary
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/d1cecbb5d7eb4fa2bf8418886ff733cc
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Therapeutic effect of bone marrow mesenchymal stem cells in a rat model of carbon tetrachloride induced liver fibrosisAt a glance of commentary

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