Catalysis of the electrochemical oxygen reduction reaction (ORR) by animal and human cells.

Animal cells from the Vero lineage and MRC5 human cells were checked for their capacity to catalyse the electrochemical oxygen reduction reaction (ORR). The Vero cells needed 72 hours' incubation to induce ORR catalysis. The cyclic voltammetry curves were clearly modified by the presence of the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Simon Guette-Marquet, Christine Roques, Alain Bergel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d1da06ec4f054c45891004f2796b759d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d1da06ec4f054c45891004f2796b759d
record_format	dspace
spelling	oai:doaj.org-article:d1da06ec4f054c45891004f2796b759d2021-11-25T05:54:21ZCatalysis of the electrochemical oxygen reduction reaction (ORR) by animal and human cells.1932-620310.1371/journal.pone.0251273https://doaj.org/article/d1da06ec4f054c45891004f2796b759d2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251273https://doaj.org/toc/1932-6203Animal cells from the Vero lineage and MRC5 human cells were checked for their capacity to catalyse the electrochemical oxygen reduction reaction (ORR). The Vero cells needed 72 hours' incubation to induce ORR catalysis. The cyclic voltammetry curves were clearly modified by the presence of the cells with a shift of ORR of 50 mV towards positive potentials and the appearance of a limiting current (59 μA.cm-2). The MRC5 cells induced considerable ORR catalysis after only 4 h of incubation with a potential shift of 110 mV but with large experimental deviation. A longer incubation time, of 24 h, made the results more reproducible with a potential shift of 90 mV. The presence of carbon nanotubes on the electrode surface or pre-treatment with foetal bovine serum or poly-D-lysine did not change the results. These data are the first demonstrations of the capability of animal and human cells to catalyse electrochemical ORR. The discussion of the possible mechanisms suggests that these pioneering observations could pave the way for electrochemical biosensors able to characterize the protective system of cells against oxidative stress and its sensitivity to external agents.Simon Guette-MarquetChristine RoquesAlain BergelPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0251273 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Simon Guette-Marquet Christine Roques Alain Bergel Catalysis of the electrochemical oxygen reduction reaction (ORR) by animal and human cells.
description	Animal cells from the Vero lineage and MRC5 human cells were checked for their capacity to catalyse the electrochemical oxygen reduction reaction (ORR). The Vero cells needed 72 hours' incubation to induce ORR catalysis. The cyclic voltammetry curves were clearly modified by the presence of the cells with a shift of ORR of 50 mV towards positive potentials and the appearance of a limiting current (59 μA.cm-2). The MRC5 cells induced considerable ORR catalysis after only 4 h of incubation with a potential shift of 110 mV but with large experimental deviation. A longer incubation time, of 24 h, made the results more reproducible with a potential shift of 90 mV. The presence of carbon nanotubes on the electrode surface or pre-treatment with foetal bovine serum or poly-D-lysine did not change the results. These data are the first demonstrations of the capability of animal and human cells to catalyse electrochemical ORR. The discussion of the possible mechanisms suggests that these pioneering observations could pave the way for electrochemical biosensors able to characterize the protective system of cells against oxidative stress and its sensitivity to external agents.
format	article
author	Simon Guette-Marquet Christine Roques Alain Bergel
author_facet	Simon Guette-Marquet Christine Roques Alain Bergel
author_sort	Simon Guette-Marquet
title	Catalysis of the electrochemical oxygen reduction reaction (ORR) by animal and human cells.
title_short	Catalysis of the electrochemical oxygen reduction reaction (ORR) by animal and human cells.
title_full	Catalysis of the electrochemical oxygen reduction reaction (ORR) by animal and human cells.
title_fullStr	Catalysis of the electrochemical oxygen reduction reaction (ORR) by animal and human cells.
title_full_unstemmed	Catalysis of the electrochemical oxygen reduction reaction (ORR) by animal and human cells.
title_sort	catalysis of the electrochemical oxygen reduction reaction (orr) by animal and human cells.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/d1da06ec4f054c45891004f2796b759d
work_keys_str_mv	AT simonguettemarquet catalysisoftheelectrochemicaloxygenreductionreactionorrbyanimalandhumancells AT christineroques catalysisoftheelectrochemicaloxygenreductionreactionorrbyanimalandhumancells AT alainbergel catalysisoftheelectrochemicaloxygenreductionreactionorrbyanimalandhumancells
_version_	1718414427524431872

Catalysis of the electrochemical oxygen reduction reaction (ORR) by animal and human cells.

Ejemplares similares