Morphological changes in the sciatic nerve in experimental modeling of contusion injury of the spinal cord in rats

Objective. To analyze morphological and morphometric changes in the sciatic nerve of rats after the spinal cord injury. Material and Methods. The Т9 moderately severe contusion injury of the spinal cord was simulated in 12 Wistar female rats. Functions of the pelvic limbs were assessed according...

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Autores principales:	Nadezhda V. Kubrak, Tatiana N. Varsegova, Sergey O. Ryabykh
Formato:	article
Lenguaje:	EN RU
Publicado:	Ministry of Health of Russian Federation, Federal State Budgetary Institution "Novosibirsk Research Institute of Traumatology and Orthopaedics n.a. Ya.L. Tsivyan" 2021
Materias:	rat contusion injury of the spinal cord functional assessment using bbb scale sciatic nerve morphology morphometry Surgery RD1-811
Acceso en línea:	https://doaj.org/article/d1e5447dbc104904a9ef0f71f84d6e25
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spelling	oai:doaj.org-article:d1e5447dbc104904a9ef0f71f84d6e252021-11-22T08:05:18ZMorphological changes in the sciatic nerve in experimental modeling of contusion injury of the spinal cord in rats10.14531/ss2021.3.36-421810-89972313-1497https://doaj.org/article/d1e5447dbc104904a9ef0f71f84d6e252021-09-01T00:00:00Zhttps://www.spinesurgery.ru/jour/article/view/1826/1823https://doaj.org/toc/1810-8997https://doaj.org/toc/2313-1497Objective. To analyze morphological and morphometric changes in the sciatic nerve of rats after the spinal cord injury. Material and Methods. The Т9 moderately severe contusion injury of the spinal cord was simulated in 12 Wistar female rats. Functions of the pelvic limbs were assessed according to the standardized BBB scale. The animals were withdrawn from the experiment after nine and 13 weeks. Epoxy semi-thin (1 μm) sections were used to study sciatic nerve at the light-optical level. Results. Significant recovery of pelvic limb functions was observed within four weeks after surgery, the plateau was achieved by Week 5 (9.5 ± 0.28 points according to the BBB scale), the deterioration in the motor activity was observed by Week 9 (8.67 ± 0.33), its recovery was achieved by Week 13 of the experiment (9.5 ± 0.87). After 9 and 13 weeks, reactive-destructive changes were detected in the sciatic nerve in 9 % and 8 % of nerve conductors, an increase in the number density of myelin fibers by 28 % and 27 % (p < 0.05) and myelin-free fibers by 20 % and 49 % (p < 0.05), and a decrease in axon diameters by 8 % and 10 % (p < 0.05), respectively. Conclusions. The morphological and morphometric changes in the sciatic nerve revealed after the spinal cord injury in the form of destruc- tion of a part of the fibers, axonal atrophy and a decrease in the proportion of large fibers negatively affect its conductive properties. The leveling of peripheral nerve damage, possibly, will accelerate the regression of the motor deficit caused by the spinal cord injury; therefore, it is necessary to develop a set of preventive measures aimed at preventing the reorganization of the peripheral nerve tissue.Nadezhda V. KubrakTatiana N. VarsegovaSergey O. RyabykhMinistry of Health of Russian Federation, Federal State Budgetary Institution "Novosibirsk Research Institute of Traumatology and Orthopaedics n.a. Ya.L. Tsivyan"articleratcontusion injury of the spinal cordfunctional assessment using bbb scalesciatic nervemorphologymorphometrySurgeryRD1-811ENRUХирургия позвоночника, Vol 18, Iss 3, Pp 36-42 (2021)
institution	DOAJ
collection	DOAJ
language	EN RU
topic	rat contusion injury of the spinal cord functional assessment using bbb scale sciatic nerve morphology morphometry Surgery RD1-811
spellingShingle	rat contusion injury of the spinal cord functional assessment using bbb scale sciatic nerve morphology morphometry Surgery RD1-811 Nadezhda V. Kubrak Tatiana N. Varsegova Sergey O. Ryabykh Morphological changes in the sciatic nerve in experimental modeling of contusion injury of the spinal cord in rats
description	Objective. To analyze morphological and morphometric changes in the sciatic nerve of rats after the spinal cord injury. Material and Methods. The Т9 moderately severe contusion injury of the spinal cord was simulated in 12 Wistar female rats. Functions of the pelvic limbs were assessed according to the standardized BBB scale. The animals were withdrawn from the experiment after nine and 13 weeks. Epoxy semi-thin (1 μm) sections were used to study sciatic nerve at the light-optical level. Results. Significant recovery of pelvic limb functions was observed within four weeks after surgery, the plateau was achieved by Week 5 (9.5 ± 0.28 points according to the BBB scale), the deterioration in the motor activity was observed by Week 9 (8.67 ± 0.33), its recovery was achieved by Week 13 of the experiment (9.5 ± 0.87). After 9 and 13 weeks, reactive-destructive changes were detected in the sciatic nerve in 9 % and 8 % of nerve conductors, an increase in the number density of myelin fibers by 28 % and 27 % (p < 0.05) and myelin-free fibers by 20 % and 49 % (p < 0.05), and a decrease in axon diameters by 8 % and 10 % (p < 0.05), respectively. Conclusions. The morphological and morphometric changes in the sciatic nerve revealed after the spinal cord injury in the form of destruc- tion of a part of the fibers, axonal atrophy and a decrease in the proportion of large fibers negatively affect its conductive properties. The leveling of peripheral nerve damage, possibly, will accelerate the regression of the motor deficit caused by the spinal cord injury; therefore, it is necessary to develop a set of preventive measures aimed at preventing the reorganization of the peripheral nerve tissue.
format	article
author	Nadezhda V. Kubrak Tatiana N. Varsegova Sergey O. Ryabykh
author_facet	Nadezhda V. Kubrak Tatiana N. Varsegova Sergey O. Ryabykh
author_sort	Nadezhda V. Kubrak
title	Morphological changes in the sciatic nerve in experimental modeling of contusion injury of the spinal cord in rats
title_short	Morphological changes in the sciatic nerve in experimental modeling of contusion injury of the spinal cord in rats
title_full	Morphological changes in the sciatic nerve in experimental modeling of contusion injury of the spinal cord in rats
title_fullStr	Morphological changes in the sciatic nerve in experimental modeling of contusion injury of the spinal cord in rats
title_full_unstemmed	Morphological changes in the sciatic nerve in experimental modeling of contusion injury of the spinal cord in rats
title_sort	morphological changes in the sciatic nerve in experimental modeling of contusion injury of the spinal cord in rats
publisher	Ministry of Health of Russian Federation, Federal State Budgetary Institution "Novosibirsk Research Institute of Traumatology and Orthopaedics n.a. Ya.L. Tsivyan"
publishDate	2021
url	https://doaj.org/article/d1e5447dbc104904a9ef0f71f84d6e25
work_keys_str_mv	AT nadezhdavkubrak morphologicalchangesinthesciaticnerveinexperimentalmodelingofcontusioninjuryofthespinalcordinrats AT tatiananvarsegova morphologicalchangesinthesciaticnerveinexperimentalmodelingofcontusioninjuryofthespinalcordinrats AT sergeyoryabykh morphologicalchangesinthesciaticnerveinexperimentalmodelingofcontusioninjuryofthespinalcordinrats
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Morphological changes in the sciatic nerve in experimental modeling of contusion injury of the spinal cord in rats

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