Complete genome sequence and comparative analysis of the fish pathogen Lactococcus garvieae.

Lactococcus garvieae causes fatal haemorrhagic septicaemia in fish such as yellowtail. The comparative analysis of genomes of a virulent strain Lg2 and a non-virulent strain ATCC 49156 of L. garvieae revealed that the two strains shared a high degree of sequence identity, but Lg2 had a 16.5-kb capsu...

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Autores principales:	Hidetoshi Morita, Hidehiro Toh, Kenshiro Oshima, Mariko Yoshizaki, Michiko Kawanishi, Kohei Nakaya, Takehito Suzuki, Eiji Miyauchi, Yasuo Ishii, Soichi Tanabe, Masaru Murakami, Masahira Hattori
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d1e684668c974d2b9792eb6c81154d8f
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spelling	oai:doaj.org-article:d1e684668c974d2b9792eb6c81154d8f2021-11-18T06:48:37ZComplete genome sequence and comparative analysis of the fish pathogen Lactococcus garvieae.1932-620310.1371/journal.pone.0023184https://doaj.org/article/d1e684668c974d2b9792eb6c81154d8f2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21829716/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Lactococcus garvieae causes fatal haemorrhagic septicaemia in fish such as yellowtail. The comparative analysis of genomes of a virulent strain Lg2 and a non-virulent strain ATCC 49156 of L. garvieae revealed that the two strains shared a high degree of sequence identity, but Lg2 had a 16.5-kb capsule gene cluster that is absent in ATCC 49156. The capsule gene cluster was composed of 15 genes, of which eight genes are highly conserved with those in exopolysaccharide biosynthesis gene cluster often found in Lactococcus lactis strains. Sequence analysis of the capsule gene cluster in the less virulent strain L. garvieae Lg2-S, Lg2-derived strain, showed that two conserved genes were disrupted by a single base pair deletion, respectively. These results strongly suggest that the capsule is crucial for virulence of Lg2. The capsule gene cluster of Lg2 may be a genomic island from several features such as the presence of insertion sequences flanked on both ends, different GC content from the chromosomal average, integration into the locus syntenic to other lactococcal genome sequences, and distribution in human gut microbiomes. The analysis also predicted other potential virulence factors such as haemolysin. The present study provides new insights into understanding of the virulence mechanisms of L. garvieae in fish.Hidetoshi MoritaHidehiro TohKenshiro OshimaMariko YoshizakiMichiko KawanishiKohei NakayaTakehito SuzukiEiji MiyauchiYasuo IshiiSoichi TanabeMasaru MurakamiMasahira HattoriPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 8, p e23184 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hidetoshi Morita Hidehiro Toh Kenshiro Oshima Mariko Yoshizaki Michiko Kawanishi Kohei Nakaya Takehito Suzuki Eiji Miyauchi Yasuo Ishii Soichi Tanabe Masaru Murakami Masahira Hattori Complete genome sequence and comparative analysis of the fish pathogen Lactococcus garvieae.
description	Lactococcus garvieae causes fatal haemorrhagic septicaemia in fish such as yellowtail. The comparative analysis of genomes of a virulent strain Lg2 and a non-virulent strain ATCC 49156 of L. garvieae revealed that the two strains shared a high degree of sequence identity, but Lg2 had a 16.5-kb capsule gene cluster that is absent in ATCC 49156. The capsule gene cluster was composed of 15 genes, of which eight genes are highly conserved with those in exopolysaccharide biosynthesis gene cluster often found in Lactococcus lactis strains. Sequence analysis of the capsule gene cluster in the less virulent strain L. garvieae Lg2-S, Lg2-derived strain, showed that two conserved genes were disrupted by a single base pair deletion, respectively. These results strongly suggest that the capsule is crucial for virulence of Lg2. The capsule gene cluster of Lg2 may be a genomic island from several features such as the presence of insertion sequences flanked on both ends, different GC content from the chromosomal average, integration into the locus syntenic to other lactococcal genome sequences, and distribution in human gut microbiomes. The analysis also predicted other potential virulence factors such as haemolysin. The present study provides new insights into understanding of the virulence mechanisms of L. garvieae in fish.
format	article
author	Hidetoshi Morita Hidehiro Toh Kenshiro Oshima Mariko Yoshizaki Michiko Kawanishi Kohei Nakaya Takehito Suzuki Eiji Miyauchi Yasuo Ishii Soichi Tanabe Masaru Murakami Masahira Hattori
author_facet	Hidetoshi Morita Hidehiro Toh Kenshiro Oshima Mariko Yoshizaki Michiko Kawanishi Kohei Nakaya Takehito Suzuki Eiji Miyauchi Yasuo Ishii Soichi Tanabe Masaru Murakami Masahira Hattori
author_sort	Hidetoshi Morita
title	Complete genome sequence and comparative analysis of the fish pathogen Lactococcus garvieae.
title_short	Complete genome sequence and comparative analysis of the fish pathogen Lactococcus garvieae.
title_full	Complete genome sequence and comparative analysis of the fish pathogen Lactococcus garvieae.
title_fullStr	Complete genome sequence and comparative analysis of the fish pathogen Lactococcus garvieae.
title_full_unstemmed	Complete genome sequence and comparative analysis of the fish pathogen Lactococcus garvieae.
title_sort	complete genome sequence and comparative analysis of the fish pathogen lactococcus garvieae.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/d1e684668c974d2b9792eb6c81154d8f
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Complete genome sequence and comparative analysis of the fish pathogen Lactococcus garvieae.

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