ZjSEP3 modulates flowering time by regulating the LHY promoter

Abstract Background SEPALLATA3 (SEP3), which is conserved across various plant species, plays essential and various roles in flower and fruit development. However, the regulatory network of the role of SEP3 in flowering time at the molecular level remained unclear. Results Here, we investigated that...

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Autores principales:	Weilin Gao, Liman Zhang, Jiurui Wang, Zhiguo Liu, Yao Zhang, Chaoling Xue, Mengjun Liu, Jin Zhao
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Ziziphus jujuba Mill. ZjSEP3 Early flowering Transcriptional factor LHY promoter CArG-box Botany QK1-989
Acceso en línea:	https://doaj.org/article/d1ea43e06df240f98ab7b00127658c2b
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spelling	oai:doaj.org-article:d1ea43e06df240f98ab7b00127658c2b2021-11-14T12:09:04ZZjSEP3 modulates flowering time by regulating the LHY promoter10.1186/s12870-021-03305-x1471-2229https://doaj.org/article/d1ea43e06df240f98ab7b00127658c2b2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12870-021-03305-xhttps://doaj.org/toc/1471-2229Abstract Background SEPALLATA3 (SEP3), which is conserved across various plant species, plays essential and various roles in flower and fruit development. However, the regulatory network of the role of SEP3 in flowering time at the molecular level remained unclear. Results Here, we investigated that SEP3 in Ziziphus jujuba Mill. (ZjSEP3) was expressed in four floral organs and exhibited strong transcriptional activation activity. ZjSEP3 transgenic Arabidopsis showed an early-flowering phenotype and altered the expression of some genes related to flowering. Among them, the expression of LATE ELONGATED HYPOCOTYL (AtLHY), the key gene of circadian rhythms, was significantly suppressed. Yeast one-hybrid (Y1H) and electrophoretic mobility shift assays (EMSAs) further verified that ZjSEP3 inhibited the transcription of AtLHY by binding to the CArG-boxes in its promoter. Moreover, ZjSEP3 also could bind to the ZjLHY promoter and the conserved binding regions of ZjSEP3 were found in the LHY promoter of various plant species. The ectopic regulatory pathway of ZjSEP3-AtLHY was further supported by the ability of 35S::AtLHY to rescue the early-flowering phenotype in ZjSEP3 transgenic plants. In ZjSEP3 transgenic plants, total chlorophyll content and the expression of genes involved in chlorophyll synthesis increased during vegetative stages, which should contribute to its early flowering and relate to the regulatory of AtLHY. Conclusion Overall, ZjSEP3-AtLHY pathway represents a novel regulatory mechanism that is involved in the regulation of flowering time.Weilin GaoLiman ZhangJiurui WangZhiguo LiuYao ZhangChaoling XueMengjun LiuJin ZhaoBMCarticleZiziphus jujuba Mill.ZjSEP3Early floweringTranscriptional factorLHY promoterCArG-boxBotanyQK1-989ENBMC Plant Biology, Vol 21, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ziziphus jujuba Mill. ZjSEP3 Early flowering Transcriptional factor LHY promoter CArG-box Botany QK1-989
spellingShingle	Ziziphus jujuba Mill. ZjSEP3 Early flowering Transcriptional factor LHY promoter CArG-box Botany QK1-989 Weilin Gao Liman Zhang Jiurui Wang Zhiguo Liu Yao Zhang Chaoling Xue Mengjun Liu Jin Zhao ZjSEP3 modulates flowering time by regulating the LHY promoter
description	Abstract Background SEPALLATA3 (SEP3), which is conserved across various plant species, plays essential and various roles in flower and fruit development. However, the regulatory network of the role of SEP3 in flowering time at the molecular level remained unclear. Results Here, we investigated that SEP3 in Ziziphus jujuba Mill. (ZjSEP3) was expressed in four floral organs and exhibited strong transcriptional activation activity. ZjSEP3 transgenic Arabidopsis showed an early-flowering phenotype and altered the expression of some genes related to flowering. Among them, the expression of LATE ELONGATED HYPOCOTYL (AtLHY), the key gene of circadian rhythms, was significantly suppressed. Yeast one-hybrid (Y1H) and electrophoretic mobility shift assays (EMSAs) further verified that ZjSEP3 inhibited the transcription of AtLHY by binding to the CArG-boxes in its promoter. Moreover, ZjSEP3 also could bind to the ZjLHY promoter and the conserved binding regions of ZjSEP3 were found in the LHY promoter of various plant species. The ectopic regulatory pathway of ZjSEP3-AtLHY was further supported by the ability of 35S::AtLHY to rescue the early-flowering phenotype in ZjSEP3 transgenic plants. In ZjSEP3 transgenic plants, total chlorophyll content and the expression of genes involved in chlorophyll synthesis increased during vegetative stages, which should contribute to its early flowering and relate to the regulatory of AtLHY. Conclusion Overall, ZjSEP3-AtLHY pathway represents a novel regulatory mechanism that is involved in the regulation of flowering time.
format	article
author	Weilin Gao Liman Zhang Jiurui Wang Zhiguo Liu Yao Zhang Chaoling Xue Mengjun Liu Jin Zhao
author_facet	Weilin Gao Liman Zhang Jiurui Wang Zhiguo Liu Yao Zhang Chaoling Xue Mengjun Liu Jin Zhao
author_sort	Weilin Gao
title	ZjSEP3 modulates flowering time by regulating the LHY promoter
title_short	ZjSEP3 modulates flowering time by regulating the LHY promoter
title_full	ZjSEP3 modulates flowering time by regulating the LHY promoter
title_fullStr	ZjSEP3 modulates flowering time by regulating the LHY promoter
title_full_unstemmed	ZjSEP3 modulates flowering time by regulating the LHY promoter
title_sort	zjsep3 modulates flowering time by regulating the lhy promoter
publisher	BMC
publishDate	2021
url	https://doaj.org/article/d1ea43e06df240f98ab7b00127658c2b
work_keys_str_mv	AT weilingao zjsep3modulatesfloweringtimebyregulatingthelhypromoter AT limanzhang zjsep3modulatesfloweringtimebyregulatingthelhypromoter AT jiuruiwang zjsep3modulatesfloweringtimebyregulatingthelhypromoter AT zhiguoliu zjsep3modulatesfloweringtimebyregulatingthelhypromoter AT yaozhang zjsep3modulatesfloweringtimebyregulatingthelhypromoter AT chaolingxue zjsep3modulatesfloweringtimebyregulatingthelhypromoter AT mengjunliu zjsep3modulatesfloweringtimebyregulatingthelhypromoter AT jinzhao zjsep3modulatesfloweringtimebyregulatingthelhypromoter
_version_	1718429433220562944

ZjSEP3 modulates flowering time by regulating the LHY promoter

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