Tumor cytotoxicity and immunogenicity of a novel V-jet neon plasma source compared to the kINPen

Abstract Recent research indicated the potential of cold physical plasma in cancer therapy. The plethora of plasma-derived reactive oxygen and nitrogen species (ROS/RNS) mediate diverse antitumor effects after eliciting oxidative stress in cancer cells. We aimed at exploiting this principle using a...

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Autores principales:	Lea Miebach, Eric Freund, Stefan Horn, Felix Niessner, Sanjeev Kumar Sagwal, Thomas von Woedtke, Steffen Emmert, Klaus-Dieter Weltmann, Ramona Clemen, Anke Schmidt, Torsten Gerling, Sander Bekeschus
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d1f4e475416d406499aee390b8c8b4e6
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spelling	oai:doaj.org-article:d1f4e475416d406499aee390b8c8b4e62021-12-02T11:45:54ZTumor cytotoxicity and immunogenicity of a novel V-jet neon plasma source compared to the kINPen10.1038/s41598-020-80512-w2045-2322https://doaj.org/article/d1f4e475416d406499aee390b8c8b4e62021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80512-whttps://doaj.org/toc/2045-2322Abstract Recent research indicated the potential of cold physical plasma in cancer therapy. The plethora of plasma-derived reactive oxygen and nitrogen species (ROS/RNS) mediate diverse antitumor effects after eliciting oxidative stress in cancer cells. We aimed at exploiting this principle using a newly designed dual-jet neon plasma source (Vjet) to treat colorectal cancer cells. A treatment time-dependent ROS/RNS generation induced oxidation, growth retardation, and cell death within 3D tumor spheroids were found. In TUM-CAM, a semi in vivo model, the Vjet markedly reduced vascularized tumors' growth, but an increase of tumor cell immunogenicity or uptake by dendritic cells was not observed. By comparison, the argon-driven single jet kINPen, known to mediate anticancer effects in vitro, in vivo, and in patients, generated less ROS/RNS and terminal cell death in spheroids. In the TUM-CAM model, however, the kINPen was equivalently effective and induced a stronger expression of immunogenic cancer cell death (ICD) markers, leading to increased phagocytosis of kINPen but not Vjet plasma-treated tumor cells by dendritic cells. Moreover, the Vjet was characterized according to the requirements of the DIN-SPEC 91315. Our results highlight the plasma device-specific action on cancer cells for evaluating optimal discharges for plasma cancer treatment.Lea MiebachEric FreundStefan HornFelix NiessnerSanjeev Kumar SagwalThomas von WoedtkeSteffen EmmertKlaus-Dieter WeltmannRamona ClemenAnke SchmidtTorsten GerlingSander BekeschusNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lea Miebach Eric Freund Stefan Horn Felix Niessner Sanjeev Kumar Sagwal Thomas von Woedtke Steffen Emmert Klaus-Dieter Weltmann Ramona Clemen Anke Schmidt Torsten Gerling Sander Bekeschus Tumor cytotoxicity and immunogenicity of a novel V-jet neon plasma source compared to the kINPen
description	Abstract Recent research indicated the potential of cold physical plasma in cancer therapy. The plethora of plasma-derived reactive oxygen and nitrogen species (ROS/RNS) mediate diverse antitumor effects after eliciting oxidative stress in cancer cells. We aimed at exploiting this principle using a newly designed dual-jet neon plasma source (Vjet) to treat colorectal cancer cells. A treatment time-dependent ROS/RNS generation induced oxidation, growth retardation, and cell death within 3D tumor spheroids were found. In TUM-CAM, a semi in vivo model, the Vjet markedly reduced vascularized tumors' growth, but an increase of tumor cell immunogenicity or uptake by dendritic cells was not observed. By comparison, the argon-driven single jet kINPen, known to mediate anticancer effects in vitro, in vivo, and in patients, generated less ROS/RNS and terminal cell death in spheroids. In the TUM-CAM model, however, the kINPen was equivalently effective and induced a stronger expression of immunogenic cancer cell death (ICD) markers, leading to increased phagocytosis of kINPen but not Vjet plasma-treated tumor cells by dendritic cells. Moreover, the Vjet was characterized according to the requirements of the DIN-SPEC 91315. Our results highlight the plasma device-specific action on cancer cells for evaluating optimal discharges for plasma cancer treatment.
format	article
author	Lea Miebach Eric Freund Stefan Horn Felix Niessner Sanjeev Kumar Sagwal Thomas von Woedtke Steffen Emmert Klaus-Dieter Weltmann Ramona Clemen Anke Schmidt Torsten Gerling Sander Bekeschus
author_facet	Lea Miebach Eric Freund Stefan Horn Felix Niessner Sanjeev Kumar Sagwal Thomas von Woedtke Steffen Emmert Klaus-Dieter Weltmann Ramona Clemen Anke Schmidt Torsten Gerling Sander Bekeschus
author_sort	Lea Miebach
title	Tumor cytotoxicity and immunogenicity of a novel V-jet neon plasma source compared to the kINPen
title_short	Tumor cytotoxicity and immunogenicity of a novel V-jet neon plasma source compared to the kINPen
title_full	Tumor cytotoxicity and immunogenicity of a novel V-jet neon plasma source compared to the kINPen
title_fullStr	Tumor cytotoxicity and immunogenicity of a novel V-jet neon plasma source compared to the kINPen
title_full_unstemmed	Tumor cytotoxicity and immunogenicity of a novel V-jet neon plasma source compared to the kINPen
title_sort	tumor cytotoxicity and immunogenicity of a novel v-jet neon plasma source compared to the kinpen
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d1f4e475416d406499aee390b8c8b4e6
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Tumor cytotoxicity and immunogenicity of a novel V-jet neon plasma source compared to the kINPen

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