Inventory of some Tulipa species from Uzbekistan using DNA barcoding

Climate fluctuations in the Quaternary period in significantly influenced the evolution and distribution of plants, which caused to a serious genetic effects and speciation activity [1]. Thus, Central Asia is distinguished by a wide variety of flora, including 9520 species, while 20% are endemics [2...

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Autores principales:	Nikitina Elena V., Karimov Farkhod I., Savina Natalja V., Kubrak Svetlana V., Kilchevsky Aleksandr V.
Formato:	article
Lenguaje:	EN FR
Publicado:	EDP Sciences 2021
Materias:	Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/d1f7d92cdef84c96961a7065202bcd6c
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id	oai:doaj.org-article:d1f7d92cdef84c96961a7065202bcd6c
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spelling	oai:doaj.org-article:d1f7d92cdef84c96961a7065202bcd6c2021-11-08T15:17:37ZInventory of some Tulipa species from Uzbekistan using DNA barcoding2117-445810.1051/bioconf/20213800086https://doaj.org/article/d1f7d92cdef84c96961a7065202bcd6c2021-01-01T00:00:00Zhttps://www.bio-conferences.org/articles/bioconf/pdf/2021/10/bioconf_napd2021_00086.pdfhttps://doaj.org/toc/2117-4458Climate fluctuations in the Quaternary period in significantly influenced the evolution and distribution of plants, which caused to a serious genetic effects and speciation activity [1]. Thus, Central Asia is distinguished by a wide variety of flora, including 9520 species, while 20% are endemics [2]. Great phenotypic diversity of the wild flora in Uzbekistan, numbering about 4400 species of vascular plants is determined by the peculiar climatic conditions, a variety of zoning, altitudinal zones [3]. At the same time, being one of the centers of early diversification of monocotyledonous geophytes, taxonomically complex representatives of this group are not sufficiently involved in phylogenetic studies. This study is a pilot study of the flora of Uzbekistan using DNA barcoding method, that based on sequencing of phylogenetically significant sequences. Four plastid (rbcL, psbA-trnH, matK, trnL-F) and one nuclear (ITS) markers were used for species identification of the genus Tulipa. The results of molecular phylogenetic analysis for 15 species of Tulipa (Liliaceae) using the marker sequence encoding ITS fragment are presented. The successful usefulness of DNA technology for genetic inventory has been demonstrated.Nikitina Elena V.Karimov Farkhod I.Savina Natalja V.Kubrak Svetlana V.Kilchevsky Aleksandr V.EDP SciencesarticleMicrobiologyQR1-502PhysiologyQP1-981ZoologyQL1-991ENFRBIO Web of Conferences, Vol 38, p 00086 (2021)
institution	DOAJ
collection	DOAJ
language	EN FR
topic	Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991
spellingShingle	Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991 Nikitina Elena V. Karimov Farkhod I. Savina Natalja V. Kubrak Svetlana V. Kilchevsky Aleksandr V. Inventory of some Tulipa species from Uzbekistan using DNA barcoding
description	Climate fluctuations in the Quaternary period in significantly influenced the evolution and distribution of plants, which caused to a serious genetic effects and speciation activity [1]. Thus, Central Asia is distinguished by a wide variety of flora, including 9520 species, while 20% are endemics [2]. Great phenotypic diversity of the wild flora in Uzbekistan, numbering about 4400 species of vascular plants is determined by the peculiar climatic conditions, a variety of zoning, altitudinal zones [3]. At the same time, being one of the centers of early diversification of monocotyledonous geophytes, taxonomically complex representatives of this group are not sufficiently involved in phylogenetic studies. This study is a pilot study of the flora of Uzbekistan using DNA barcoding method, that based on sequencing of phylogenetically significant sequences. Four plastid (rbcL, psbA-trnH, matK, trnL-F) and one nuclear (ITS) markers were used for species identification of the genus Tulipa. The results of molecular phylogenetic analysis for 15 species of Tulipa (Liliaceae) using the marker sequence encoding ITS fragment are presented. The successful usefulness of DNA technology for genetic inventory has been demonstrated.
format	article
author	Nikitina Elena V. Karimov Farkhod I. Savina Natalja V. Kubrak Svetlana V. Kilchevsky Aleksandr V.
author_facet	Nikitina Elena V. Karimov Farkhod I. Savina Natalja V. Kubrak Svetlana V. Kilchevsky Aleksandr V.
author_sort	Nikitina Elena V.
title	Inventory of some Tulipa species from Uzbekistan using DNA barcoding
title_short	Inventory of some Tulipa species from Uzbekistan using DNA barcoding
title_full	Inventory of some Tulipa species from Uzbekistan using DNA barcoding
title_fullStr	Inventory of some Tulipa species from Uzbekistan using DNA barcoding
title_full_unstemmed	Inventory of some Tulipa species from Uzbekistan using DNA barcoding
title_sort	inventory of some tulipa species from uzbekistan using dna barcoding
publisher	EDP Sciences
publishDate	2021
url	https://doaj.org/article/d1f7d92cdef84c96961a7065202bcd6c
work_keys_str_mv	AT nikitinaelenav inventoryofsometulipaspeciesfromuzbekistanusingdnabarcoding AT karimovfarkhodi inventoryofsometulipaspeciesfromuzbekistanusingdnabarcoding AT savinanataljav inventoryofsometulipaspeciesfromuzbekistanusingdnabarcoding AT kubraksvetlanav inventoryofsometulipaspeciesfromuzbekistanusingdnabarcoding AT kilchevskyaleksandrv inventoryofsometulipaspeciesfromuzbekistanusingdnabarcoding
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Inventory of some Tulipa species from Uzbekistan using DNA barcoding

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