In vitro bactericidal and imipenem synergistic effect of nano-silver against multiple drug-resistant Pseudomonas aeruginosa

Background: Pseudomonas aeruginosa (P. aeruginosa) is an aerobic gram-negative pathogen. It induces decline of lung functions and increase mortality rate. It has a high antimicrobial resistance rate limiting number of antibiotics which can be used. Objectives: Evaluation of bactericidal and antibiot...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autor principal:	Sulaiman A. Al Yousef
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Nano-silver Pseudomonas aeruginosa Imipenem Drug resistant Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/d200103d965e432bade9aca269b56580
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d200103d965e432bade9aca269b56580
record_format	dspace
spelling	oai:doaj.org-article:d200103d965e432bade9aca269b565802021-11-26T04:24:22ZIn vitro bactericidal and imipenem synergistic effect of nano-silver against multiple drug-resistant Pseudomonas aeruginosa1018-364710.1016/j.jksus.2021.101706https://doaj.org/article/d200103d965e432bade9aca269b565802022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1018364721003682https://doaj.org/toc/1018-3647Background: Pseudomonas aeruginosa (P. aeruginosa) is an aerobic gram-negative pathogen. It induces decline of lung functions and increase mortality rate. It has a high antimicrobial resistance rate limiting number of antibiotics which can be used. Objectives: Evaluation of bactericidal and antibiotic synergistic effect of Nano-silver (Ag-NP) against Multiple drug-resistant P.aeruginosa (MDR P. aeruginosa). Material and methods: P.aeruginosa were isolated from clinical specimens. MicroScan WalkAway-96SI System was used for laboratory Identification, antimicrobial susceptibility. Minimal inhibitory concentration (MIC) was determined by a microdilution method. Time kill assay determined by incubation MRSA with different concentrations of Ag-NP (0, 50, 100, and 200 μg/ml) in a shaking incubator at 37 °C for 24 h. Growth curves of bacterial cell cultures were attained through repeated measures of the optical density (O.D.) at 600 nm. The disc diffusion method was used to evaluate synergistic of Ag-NP with antibiotics. Results: The MIC value of Ag-NPs against MDR P. aeruginosa was in at of 5 μg/ml. The bacterial growths of cells treated with 1.25, 2.5, 5 and 10 µg/ml Ag-NPs were inhibited. After 4 h, almost all treated bacterial cells were dead. All of the combinations showed significant synergistic effect (P-value 0.003), and the result showed the highest synergism at concentrations of Imipenem at MIC (32 µg/ml) and 16 µg/ml. Conclusion: Nano-Silver has high therapeutic activity against MDR P. aeruginosa, it can be suggested as an alternative or adjuvant with antibiotics for MDR P. aeruginosa treatment. Further studies are required for understand synergistic effect of Ag-NP combining, and assessment of its safety.Sulaiman A. Al YousefElsevierarticleNano-silverPseudomonas aeruginosaImipenemDrug resistantScience (General)Q1-390ENJournal of King Saud University: Science, Vol 34, Iss 1, Pp 101706- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nano-silver Pseudomonas aeruginosa Imipenem Drug resistant Science (General) Q1-390
spellingShingle	Nano-silver Pseudomonas aeruginosa Imipenem Drug resistant Science (General) Q1-390 Sulaiman A. Al Yousef In vitro bactericidal and imipenem synergistic effect of nano-silver against multiple drug-resistant Pseudomonas aeruginosa
description	Background: Pseudomonas aeruginosa (P. aeruginosa) is an aerobic gram-negative pathogen. It induces decline of lung functions and increase mortality rate. It has a high antimicrobial resistance rate limiting number of antibiotics which can be used. Objectives: Evaluation of bactericidal and antibiotic synergistic effect of Nano-silver (Ag-NP) against Multiple drug-resistant P.aeruginosa (MDR P. aeruginosa). Material and methods: P.aeruginosa were isolated from clinical specimens. MicroScan WalkAway-96SI System was used for laboratory Identification, antimicrobial susceptibility. Minimal inhibitory concentration (MIC) was determined by a microdilution method. Time kill assay determined by incubation MRSA with different concentrations of Ag-NP (0, 50, 100, and 200 μg/ml) in a shaking incubator at 37 °C for 24 h. Growth curves of bacterial cell cultures were attained through repeated measures of the optical density (O.D.) at 600 nm. The disc diffusion method was used to evaluate synergistic of Ag-NP with antibiotics. Results: The MIC value of Ag-NPs against MDR P. aeruginosa was in at of 5 μg/ml. The bacterial growths of cells treated with 1.25, 2.5, 5 and 10 µg/ml Ag-NPs were inhibited. After 4 h, almost all treated bacterial cells were dead. All of the combinations showed significant synergistic effect (P-value 0.003), and the result showed the highest synergism at concentrations of Imipenem at MIC (32 µg/ml) and 16 µg/ml. Conclusion: Nano-Silver has high therapeutic activity against MDR P. aeruginosa, it can be suggested as an alternative or adjuvant with antibiotics for MDR P. aeruginosa treatment. Further studies are required for understand synergistic effect of Ag-NP combining, and assessment of its safety.
format	article
author	Sulaiman A. Al Yousef
author_facet	Sulaiman A. Al Yousef
author_sort	Sulaiman A. Al Yousef
title	In vitro bactericidal and imipenem synergistic effect of nano-silver against multiple drug-resistant Pseudomonas aeruginosa
title_short	In vitro bactericidal and imipenem synergistic effect of nano-silver against multiple drug-resistant Pseudomonas aeruginosa
title_full	In vitro bactericidal and imipenem synergistic effect of nano-silver against multiple drug-resistant Pseudomonas aeruginosa
title_fullStr	In vitro bactericidal and imipenem synergistic effect of nano-silver against multiple drug-resistant Pseudomonas aeruginosa
title_full_unstemmed	In vitro bactericidal and imipenem synergistic effect of nano-silver against multiple drug-resistant Pseudomonas aeruginosa
title_sort	in vitro bactericidal and imipenem synergistic effect of nano-silver against multiple drug-resistant pseudomonas aeruginosa
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/d200103d965e432bade9aca269b56580
work_keys_str_mv	AT sulaimanaalyousef invitrobactericidalandimipenemsynergisticeffectofnanosilveragainstmultipledrugresistantpseudomonasaeruginosa
_version_	1718409930680041472

In vitro bactericidal and imipenem synergistic effect of nano-silver against multiple drug-resistant Pseudomonas aeruginosa

Ejemplares similares