CircNFIC Balances Inflammation and Apoptosis by Sponging miR-30e-3p and Regulating DENND1B Expression
Disordered inflammation and apoptosis are closely related to diseases, and inflammation can also promote cell apoptosis, where growing evidence has shown that circular RNAs (circRNAs) play important roles. Lipopolysaccharide (LPS) is the main component of the cytoderm of gram-negative bacterium, whi...
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2021
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oai:doaj.org-article:d202f3fe0c184dabb92441819295c0802021-11-25T17:42:28ZCircNFIC Balances Inflammation and Apoptosis by Sponging miR-30e-3p and Regulating DENND1B Expression10.3390/genes121118292073-4425https://doaj.org/article/d202f3fe0c184dabb92441819295c0802021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1829https://doaj.org/toc/2073-4425Disordered inflammation and apoptosis are closely related to diseases, and inflammation can also promote cell apoptosis, where growing evidence has shown that circular RNAs (circRNAs) play important roles. Lipopolysaccharide (LPS) is the main component of the cytoderm of gram-negative bacterium, which can cause inflammatory responses in macrophages. We constructed an inflammatory model by exposing chicken macrophage cell lines (also known as HD11) to LPS for in vitro experiments. In this study, we validated a novel circRNA—circNFIC—which was dramatically up-regulated in tissues infected by coccidia and cells exposed to LPS. Besides, circNFIC could significantly promote the expression levels of pro-inflammation factors, including (IL-1β, TNFα, and IFNγ) and pro-apoptosis maker genes (caspase 3 and caspase 8) in HD11 exposed to LPS or not. In terms of mechanism, circNFIC exerted notable effects on DENND1B to regulate cell inflammation and apoptosis by sponging miR-30e-3p. The molecular functions played by miR-30e-3p and DENND1B have been explored, respectively. In addition, the effects of circNFIC knockdown suppressing the expression of pro-inflammatory and pro-apoptosis functions could be reversed by a miR-30e-3p inhibitor. On the whole, circNFIC promoted cell inflammation and apoptosis via the miR-30e-3p/DENND1B axis.Yangfeng ChenZhijun WangXiaolan ChenXi PengQinghua NieMDPI AGarticleinflammationapoptosiscircular RNAmiR-30e-3p/DENND1B axisLipopolysaccharideGeneticsQH426-470ENGenes, Vol 12, Iss 1829, p 1829 (2021) |
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DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
inflammation apoptosis circular RNA miR-30e-3p/DENND1B axis Lipopolysaccharide Genetics QH426-470 |
| spellingShingle |
inflammation apoptosis circular RNA miR-30e-3p/DENND1B axis Lipopolysaccharide Genetics QH426-470 Yangfeng Chen Zhijun Wang Xiaolan Chen Xi Peng Qinghua Nie CircNFIC Balances Inflammation and Apoptosis by Sponging miR-30e-3p and Regulating DENND1B Expression |
| description |
Disordered inflammation and apoptosis are closely related to diseases, and inflammation can also promote cell apoptosis, where growing evidence has shown that circular RNAs (circRNAs) play important roles. Lipopolysaccharide (LPS) is the main component of the cytoderm of gram-negative bacterium, which can cause inflammatory responses in macrophages. We constructed an inflammatory model by exposing chicken macrophage cell lines (also known as HD11) to LPS for in vitro experiments. In this study, we validated a novel circRNA—circNFIC—which was dramatically up-regulated in tissues infected by coccidia and cells exposed to LPS. Besides, circNFIC could significantly promote the expression levels of pro-inflammation factors, including (IL-1β, TNFα, and IFNγ) and pro-apoptosis maker genes (caspase 3 and caspase 8) in HD11 exposed to LPS or not. In terms of mechanism, circNFIC exerted notable effects on DENND1B to regulate cell inflammation and apoptosis by sponging miR-30e-3p. The molecular functions played by miR-30e-3p and DENND1B have been explored, respectively. In addition, the effects of circNFIC knockdown suppressing the expression of pro-inflammatory and pro-apoptosis functions could be reversed by a miR-30e-3p inhibitor. On the whole, circNFIC promoted cell inflammation and apoptosis via the miR-30e-3p/DENND1B axis. |
| format |
article |
| author |
Yangfeng Chen Zhijun Wang Xiaolan Chen Xi Peng Qinghua Nie |
| author_facet |
Yangfeng Chen Zhijun Wang Xiaolan Chen Xi Peng Qinghua Nie |
| author_sort |
Yangfeng Chen |
| title |
CircNFIC Balances Inflammation and Apoptosis by Sponging miR-30e-3p and Regulating DENND1B Expression |
| title_short |
CircNFIC Balances Inflammation and Apoptosis by Sponging miR-30e-3p and Regulating DENND1B Expression |
| title_full |
CircNFIC Balances Inflammation and Apoptosis by Sponging miR-30e-3p and Regulating DENND1B Expression |
| title_fullStr |
CircNFIC Balances Inflammation and Apoptosis by Sponging miR-30e-3p and Regulating DENND1B Expression |
| title_full_unstemmed |
CircNFIC Balances Inflammation and Apoptosis by Sponging miR-30e-3p and Regulating DENND1B Expression |
| title_sort |
circnfic balances inflammation and apoptosis by sponging mir-30e-3p and regulating dennd1b expression |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/d202f3fe0c184dabb92441819295c080 |
| work_keys_str_mv |
AT yangfengchen circnficbalancesinflammationandapoptosisbyspongingmir30e3pandregulatingdennd1bexpression AT zhijunwang circnficbalancesinflammationandapoptosisbyspongingmir30e3pandregulatingdennd1bexpression AT xiaolanchen circnficbalancesinflammationandapoptosisbyspongingmir30e3pandregulatingdennd1bexpression AT xipeng circnficbalancesinflammationandapoptosisbyspongingmir30e3pandregulatingdennd1bexpression AT qinghuanie circnficbalancesinflammationandapoptosisbyspongingmir30e3pandregulatingdennd1bexpression |
| _version_ |
1718412133308301312 |