Coherent fluctuation nephelometry in clinical microbiology
In this article data concerning coherent fluctuation nephelometry (CFN) use in clinical microbiology is presented. CFN-analyzer allows to solve two important problems – fast urine screening for bacteriuria within 2-4 hours and antibiotic susceptibility testing within 3-6 hours. Altogether more than...
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Sankt-Peterburg : NIIÈM imeni Pastera
2019
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oai:doaj.org-article:d2124ebb25d44c5682e166d3af08707a2021-11-22T07:09:53ZCoherent fluctuation nephelometry in clinical microbiology2220-76192313-739810.15789/2220-7619-2019-2-385-392https://doaj.org/article/d2124ebb25d44c5682e166d3af08707a2019-07-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/647https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398In this article data concerning coherent fluctuation nephelometry (CFN) use in clinical microbiology is presented. CFN-analyzer allows to solve two important problems – fast urine screening for bacteriuria within 2-4 hours and antibiotic susceptibility testing within 3-6 hours. Altogether more than 650 urine samples were tested, and the effectivity of CFN-analyzer for preliminary selection of samples for further analysis was shown. Method allows to detect negative samples, reducing the number of urine analyses by 70-80%. Simultaneous analysis of growth curves and concentration of microorganisms shows high sensitivity and specificity (95.2% и 96.9%). Also more than 250 antibiotic susceptibility tests were performed using CFN-analyzer to show its effectiveness for determination of resistant properties of both pure cultures and urine microflora without isolation of bacteria. The agreement with traditional methods was from 84% to 88%. The use of CFN-analyzer with express methods of identification of microorganisms (chromogenic nutrient broths or mass-spectrometry) allows to make full urine analysis within 1-2 days. In the future CFN-analyzer gives an opportunity to screen different human biological liquids, and finds an application for other microbiological tasks, including standardization and speeding-up in sanitary bacteriology.A. S. Gur’evO. Yu. ShalatovaE. V. RusanovaI. V. VasilenkoA. Yu. VolkovSankt-Peterburg : NIIÈM imeni Pasteraarticlecoherent fluctuation nephelometrybacteriuriascreeningurine antibiotic susceptibility testingmicrobiology analyzerInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 9, Iss 2, Pp 385-392 (2019) |
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DOAJ |
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coherent fluctuation nephelometry bacteriuria screening urine antibiotic susceptibility testing microbiology analyzer Infectious and parasitic diseases RC109-216 |
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coherent fluctuation nephelometry bacteriuria screening urine antibiotic susceptibility testing microbiology analyzer Infectious and parasitic diseases RC109-216 A. S. Gur’ev O. Yu. Shalatova E. V. Rusanova I. V. Vasilenko A. Yu. Volkov Coherent fluctuation nephelometry in clinical microbiology |
| description |
In this article data concerning coherent fluctuation nephelometry (CFN) use in clinical microbiology is presented. CFN-analyzer allows to solve two important problems – fast urine screening for bacteriuria within 2-4 hours and antibiotic susceptibility testing within 3-6 hours. Altogether more than 650 urine samples were tested, and the effectivity of CFN-analyzer for preliminary selection of samples for further analysis was shown. Method allows to detect negative samples, reducing the number of urine analyses by 70-80%. Simultaneous analysis of growth curves and concentration of microorganisms shows high sensitivity and specificity (95.2% и 96.9%). Also more than 250 antibiotic susceptibility tests were performed using CFN-analyzer to show its effectiveness for determination of resistant properties of both pure cultures and urine microflora without isolation of bacteria. The agreement with traditional methods was from 84% to 88%. The use of CFN-analyzer with express methods of identification of microorganisms (chromogenic nutrient broths or mass-spectrometry) allows to make full urine analysis within 1-2 days. In the future CFN-analyzer gives an opportunity to screen different human biological liquids, and finds an application for other microbiological tasks, including standardization and speeding-up in sanitary bacteriology. |
| format |
article |
| author |
A. S. Gur’ev O. Yu. Shalatova E. V. Rusanova I. V. Vasilenko A. Yu. Volkov |
| author_facet |
A. S. Gur’ev O. Yu. Shalatova E. V. Rusanova I. V. Vasilenko A. Yu. Volkov |
| author_sort |
A. S. Gur’ev |
| title |
Coherent fluctuation nephelometry in clinical microbiology |
| title_short |
Coherent fluctuation nephelometry in clinical microbiology |
| title_full |
Coherent fluctuation nephelometry in clinical microbiology |
| title_fullStr |
Coherent fluctuation nephelometry in clinical microbiology |
| title_full_unstemmed |
Coherent fluctuation nephelometry in clinical microbiology |
| title_sort |
coherent fluctuation nephelometry in clinical microbiology |
| publisher |
Sankt-Peterburg : NIIÈM imeni Pastera |
| publishDate |
2019 |
| url |
https://doaj.org/article/d2124ebb25d44c5682e166d3af08707a |
| work_keys_str_mv |
AT asgurev coherentfluctuationnephelometryinclinicalmicrobiology AT oyushalatova coherentfluctuationnephelometryinclinicalmicrobiology AT evrusanova coherentfluctuationnephelometryinclinicalmicrobiology AT ivvasilenko coherentfluctuationnephelometryinclinicalmicrobiology AT ayuvolkov coherentfluctuationnephelometryinclinicalmicrobiology |
| _version_ |
1718417945548292096 |