Selection and characterization of siderophores of pathogenic Escherichia coli intestinal and extraintestinal isolates

Microorganisms adopt a unique mechanism for sequestration and transport of bound iron by producing iron-chelating molecules called siderophores. Siderophores play an important role in colonization of microbes in iron-deficient sites of the host, hence acting as an important virulence factor and prom...

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Autores principales:	Kumar Gulshan, Kumar Yashwant, Kumar Gaurav, Tahlan Ajay Kumar
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	siderotyping expec ehec/stec hydroxamate siderophore catecholate siderophore Agriculture S Agriculture (General) S1-972
Acceso en línea:	https://doaj.org/article/d212f774d1084aa9ad923795d5b7d549
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spelling	oai:doaj.org-article:d212f774d1084aa9ad923795d5b7d5492021-12-05T14:10:58ZSelection and characterization of siderophores of pathogenic Escherichia coli intestinal and extraintestinal isolates2391-953110.1515/opag-2020-0104https://doaj.org/article/d212f774d1084aa9ad923795d5b7d5492021-07-01T00:00:00Zhttps://doi.org/10.1515/opag-2020-0104https://doaj.org/toc/2391-9531Microorganisms adopt a unique mechanism for sequestration and transport of bound iron by producing iron-chelating molecules called siderophores. Siderophores play an important role in colonization of microbes in iron-deficient sites of the host, hence acting as an important virulence factor and promising carriers of antimicrobials to target sites inside the microbial cells. The research was conducted to identify and characterize the siderophores of pathogenic Escherichia coli isolates obtained from different parts of India. A total of 534 confirmed E. coli isolates were tested for the expression of siderophores using chrome azurol S agar method and its type were determined by Csaky and Arnow assays for hydroxamate and catecholate-type siderophores, respectively. The obtained results revealed a difference in siderophore-type expression by intestinal and extraintestinal pathogenic E. coli. On chrome azurol S agar 45.1% of pathogenic E. coli. were found to produce siderophores, 12.5% isolates were found to produce catechol-type siderophore and 35.4% isolates produced hydroxamate-type siderophore; and isolates could be classified into five sidero-types (1) only hydroxamate producing (2) only catechol producing (3) both catechol and hydroxamate producing (4) producing siderophores other than hydroxamate and catechol types and (5) siderophore non-producers. Siderophore production was detected in isolates from all geographical regions and in all seasons of the year. The findings conclude that siderophore production is one of the important virulence and epidemiological markers of intestinal as well as extraintestinal pathogenic E. coli.Kumar GulshanKumar YashwantKumar GauravTahlan Ajay KumarDe Gruyterarticlesiderotypingexpecehec/stechydroxamate siderophorecatecholate siderophoreAgricultureSAgriculture (General)S1-972ENOpen Agriculture, Vol 6, Iss 1, Pp 456-465 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	siderotyping expec ehec/stec hydroxamate siderophore catecholate siderophore Agriculture S Agriculture (General) S1-972
spellingShingle	siderotyping expec ehec/stec hydroxamate siderophore catecholate siderophore Agriculture S Agriculture (General) S1-972 Kumar Gulshan Kumar Yashwant Kumar Gaurav Tahlan Ajay Kumar Selection and characterization of siderophores of pathogenic Escherichia coli intestinal and extraintestinal isolates
description	Microorganisms adopt a unique mechanism for sequestration and transport of bound iron by producing iron-chelating molecules called siderophores. Siderophores play an important role in colonization of microbes in iron-deficient sites of the host, hence acting as an important virulence factor and promising carriers of antimicrobials to target sites inside the microbial cells. The research was conducted to identify and characterize the siderophores of pathogenic Escherichia coli isolates obtained from different parts of India. A total of 534 confirmed E. coli isolates were tested for the expression of siderophores using chrome azurol S agar method and its type were determined by Csaky and Arnow assays for hydroxamate and catecholate-type siderophores, respectively. The obtained results revealed a difference in siderophore-type expression by intestinal and extraintestinal pathogenic E. coli. On chrome azurol S agar 45.1% of pathogenic E. coli. were found to produce siderophores, 12.5% isolates were found to produce catechol-type siderophore and 35.4% isolates produced hydroxamate-type siderophore; and isolates could be classified into five sidero-types (1) only hydroxamate producing (2) only catechol producing (3) both catechol and hydroxamate producing (4) producing siderophores other than hydroxamate and catechol types and (5) siderophore non-producers. Siderophore production was detected in isolates from all geographical regions and in all seasons of the year. The findings conclude that siderophore production is one of the important virulence and epidemiological markers of intestinal as well as extraintestinal pathogenic E. coli.
format	article
author	Kumar Gulshan Kumar Yashwant Kumar Gaurav Tahlan Ajay Kumar
author_facet	Kumar Gulshan Kumar Yashwant Kumar Gaurav Tahlan Ajay Kumar
author_sort	Kumar Gulshan
title	Selection and characterization of siderophores of pathogenic Escherichia coli intestinal and extraintestinal isolates
title_short	Selection and characterization of siderophores of pathogenic Escherichia coli intestinal and extraintestinal isolates
title_full	Selection and characterization of siderophores of pathogenic Escherichia coli intestinal and extraintestinal isolates
title_fullStr	Selection and characterization of siderophores of pathogenic Escherichia coli intestinal and extraintestinal isolates
title_full_unstemmed	Selection and characterization of siderophores of pathogenic Escherichia coli intestinal and extraintestinal isolates
title_sort	selection and characterization of siderophores of pathogenic escherichia coli intestinal and extraintestinal isolates
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/d212f774d1084aa9ad923795d5b7d549
work_keys_str_mv	AT kumargulshan selectionandcharacterizationofsiderophoresofpathogenicescherichiacoliintestinalandextraintestinalisolates AT kumaryashwant selectionandcharacterizationofsiderophoresofpathogenicescherichiacoliintestinalandextraintestinalisolates AT kumargaurav selectionandcharacterizationofsiderophoresofpathogenicescherichiacoliintestinalandextraintestinalisolates AT tahlanajaykumar selectionandcharacterizationofsiderophoresofpathogenicescherichiacoliintestinalandextraintestinalisolates
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Selection and characterization of siderophores of pathogenic Escherichia coli intestinal and extraintestinal isolates

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