Carbon ion radiation inhibits glioma and endothelial cell migration induced by secreted VEGF.

This study evaluated the effects of carbon ion and X-ray radiation and the tumor microenvironment on the migration of glioma and endothelial cells, a key process in tumorigenesis and angiogenesis during cancer progression. C6 glioma and human microvascular endothelial cells were treated with conditi...

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Autores principales:	Yang Liu, Yuanyuan Liu, Chao Sun, Lu Gan, Luwei Zhang, Aihong Mao, Yuting Du, Rong Zhou, Hong Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d227a6ef965d427284f3a59f6da48083
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spelling	oai:doaj.org-article:d227a6ef965d427284f3a59f6da480832021-11-18T08:17:19ZCarbon ion radiation inhibits glioma and endothelial cell migration induced by secreted VEGF.1932-620310.1371/journal.pone.0098448https://doaj.org/article/d227a6ef965d427284f3a59f6da480832014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24893038/?tool=EBIhttps://doaj.org/toc/1932-6203This study evaluated the effects of carbon ion and X-ray radiation and the tumor microenvironment on the migration of glioma and endothelial cells, a key process in tumorigenesis and angiogenesis during cancer progression. C6 glioma and human microvascular endothelial cells were treated with conditioned medium from cultures of glioma cells irradiated at a range of doses and the migration of both cell types, tube formation by endothelial cells, as well as the expression and secretion of migration-related proteins were evaluated. Exposure to X-ray radiation-conditioned medium induced dose-dependent increases in cell migration and tube formation, which were accompanied by an upregulation of vascular endothelial growth factor (VEGF) and matrix metalloproteinase (MMP)-2 and -9 expression. However, glioma cells treated with conditioned medium of cells irradiated at a carbon ion dose of 4.0 Gy showed a marked decrease in migratory potential and VEGF secretion relative to non-irradiated cells. The application of recombinant VEGF165 stimulated migration in glioma and endothelial cells, which was associated with increased FAK phosphorylation at Tyr861, suggesting that the suppression of cell migration by carbon ion radiation could be via VEGF-activated FAK signaling. Taken together, these findings indicate that carbon ion may be superior to X-ray radiation for inhibiting tumorigenesis and angiogenesis through modulation of VEGF level in the glioma microenvironment.Yang LiuYuanyuan LiuChao SunLu GanLuwei ZhangAihong MaoYuting DuRong ZhouHong ZhangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e98448 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yang Liu Yuanyuan Liu Chao Sun Lu Gan Luwei Zhang Aihong Mao Yuting Du Rong Zhou Hong Zhang Carbon ion radiation inhibits glioma and endothelial cell migration induced by secreted VEGF.
description	This study evaluated the effects of carbon ion and X-ray radiation and the tumor microenvironment on the migration of glioma and endothelial cells, a key process in tumorigenesis and angiogenesis during cancer progression. C6 glioma and human microvascular endothelial cells were treated with conditioned medium from cultures of glioma cells irradiated at a range of doses and the migration of both cell types, tube formation by endothelial cells, as well as the expression and secretion of migration-related proteins were evaluated. Exposure to X-ray radiation-conditioned medium induced dose-dependent increases in cell migration and tube formation, which were accompanied by an upregulation of vascular endothelial growth factor (VEGF) and matrix metalloproteinase (MMP)-2 and -9 expression. However, glioma cells treated with conditioned medium of cells irradiated at a carbon ion dose of 4.0 Gy showed a marked decrease in migratory potential and VEGF secretion relative to non-irradiated cells. The application of recombinant VEGF165 stimulated migration in glioma and endothelial cells, which was associated with increased FAK phosphorylation at Tyr861, suggesting that the suppression of cell migration by carbon ion radiation could be via VEGF-activated FAK signaling. Taken together, these findings indicate that carbon ion may be superior to X-ray radiation for inhibiting tumorigenesis and angiogenesis through modulation of VEGF level in the glioma microenvironment.
format	article
author	Yang Liu Yuanyuan Liu Chao Sun Lu Gan Luwei Zhang Aihong Mao Yuting Du Rong Zhou Hong Zhang
author_facet	Yang Liu Yuanyuan Liu Chao Sun Lu Gan Luwei Zhang Aihong Mao Yuting Du Rong Zhou Hong Zhang
author_sort	Yang Liu
title	Carbon ion radiation inhibits glioma and endothelial cell migration induced by secreted VEGF.
title_short	Carbon ion radiation inhibits glioma and endothelial cell migration induced by secreted VEGF.
title_full	Carbon ion radiation inhibits glioma and endothelial cell migration induced by secreted VEGF.
title_fullStr	Carbon ion radiation inhibits glioma and endothelial cell migration induced by secreted VEGF.
title_full_unstemmed	Carbon ion radiation inhibits glioma and endothelial cell migration induced by secreted VEGF.
title_sort	carbon ion radiation inhibits glioma and endothelial cell migration induced by secreted vegf.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/d227a6ef965d427284f3a59f6da48083
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_version_	1718421953085177856

Carbon ion radiation inhibits glioma and endothelial cell migration induced by secreted VEGF.

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