Upregulation of Mir342 in Diet-Induced Obesity Mouse and the Hypothalamic Appetite Control

In obesity and type 2 diabetes, numerous genes are differentially expressed, and microRNAs are involved in transcriptional regulation of target mRNAs, but miRNAs critically involved in the appetite control are not known. Here, we identified upregulation of miR-342-3p and its host gene Evl in brain a...

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Autores principales:	Dongxiao Zhang, Satoshi Yamaguchi, Xinhao Zhang, Boxuan Yang, Naoko Kurooka, Ryosuke Sugawara, Haya Hamed H. Albuayjan, Atsuko Nakatsuka, Jun Eguchi, Takeshi Y. Hiyama, Atsunori Kamiya, Jun Wada
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	abdominal obesity non-coding RNAs adipose tissues appetite regulation hypothalamus Diseases of the endocrine glands. Clinical endocrinology RC648-665
Acceso en línea:	https://doaj.org/article/d22838d67bcb410ca3e064ee7ca80218
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spelling	oai:doaj.org-article:d22838d67bcb410ca3e064ee7ca802182021-12-01T09:16:36ZUpregulation of Mir342 in Diet-Induced Obesity Mouse and the Hypothalamic Appetite Control1664-239210.3389/fendo.2021.727915https://doaj.org/article/d22838d67bcb410ca3e064ee7ca802182021-08-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fendo.2021.727915/fullhttps://doaj.org/toc/1664-2392In obesity and type 2 diabetes, numerous genes are differentially expressed, and microRNAs are involved in transcriptional regulation of target mRNAs, but miRNAs critically involved in the appetite control are not known. Here, we identified upregulation of miR-342-3p and its host gene Evl in brain and adipose tissues in C57BL/6 mice fed with high fat-high sucrose (HFHS) chow by RNA sequencing. Mir342 (-/-) mice fed with HFHS chow were protected from obesity and diabetes. The hypothalamic arcuate nucleus neurons co-express Mir342 and EVL. The percentage of activated NPY+pSTAT3+ neurons were reduced, while POMC+pSTAT3+ neurons increased in Mir342 (-/-) mice, and they demonstrated the reduction of food intake and amelioration of metabolic phenotypes. Snap25 was identified as a major target gene of miR-342-3p and the reduced expression of Snap25 may link to functional impairment hypothalamic neurons and excess of food intake. The inhibition of miR-342-3p may be a potential candidate for miRNA-based therapy.Dongxiao ZhangSatoshi YamaguchiXinhao ZhangBoxuan YangNaoko KurookaRyosuke SugawaraHaya Hamed H. AlbuayjanAtsuko NakatsukaJun EguchiTakeshi Y. HiyamaAtsunori KamiyaJun WadaFrontiers Media S.A.articleabdominal obesitynon-coding RNAsadipose tissuesappetite regulationhypothalamusDiseases of the endocrine glands. Clinical endocrinologyRC648-665ENFrontiers in Endocrinology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	abdominal obesity non-coding RNAs adipose tissues appetite regulation hypothalamus Diseases of the endocrine glands. Clinical endocrinology RC648-665
spellingShingle	abdominal obesity non-coding RNAs adipose tissues appetite regulation hypothalamus Diseases of the endocrine glands. Clinical endocrinology RC648-665 Dongxiao Zhang Satoshi Yamaguchi Xinhao Zhang Boxuan Yang Naoko Kurooka Ryosuke Sugawara Haya Hamed H. Albuayjan Atsuko Nakatsuka Jun Eguchi Takeshi Y. Hiyama Atsunori Kamiya Jun Wada Upregulation of Mir342 in Diet-Induced Obesity Mouse and the Hypothalamic Appetite Control
description	In obesity and type 2 diabetes, numerous genes are differentially expressed, and microRNAs are involved in transcriptional regulation of target mRNAs, but miRNAs critically involved in the appetite control are not known. Here, we identified upregulation of miR-342-3p and its host gene Evl in brain and adipose tissues in C57BL/6 mice fed with high fat-high sucrose (HFHS) chow by RNA sequencing. Mir342 (-/-) mice fed with HFHS chow were protected from obesity and diabetes. The hypothalamic arcuate nucleus neurons co-express Mir342 and EVL. The percentage of activated NPY+pSTAT3+ neurons were reduced, while POMC+pSTAT3+ neurons increased in Mir342 (-/-) mice, and they demonstrated the reduction of food intake and amelioration of metabolic phenotypes. Snap25 was identified as a major target gene of miR-342-3p and the reduced expression of Snap25 may link to functional impairment hypothalamic neurons and excess of food intake. The inhibition of miR-342-3p may be a potential candidate for miRNA-based therapy.
format	article
author	Dongxiao Zhang Satoshi Yamaguchi Xinhao Zhang Boxuan Yang Naoko Kurooka Ryosuke Sugawara Haya Hamed H. Albuayjan Atsuko Nakatsuka Jun Eguchi Takeshi Y. Hiyama Atsunori Kamiya Jun Wada
author_facet	Dongxiao Zhang Satoshi Yamaguchi Xinhao Zhang Boxuan Yang Naoko Kurooka Ryosuke Sugawara Haya Hamed H. Albuayjan Atsuko Nakatsuka Jun Eguchi Takeshi Y. Hiyama Atsunori Kamiya Jun Wada
author_sort	Dongxiao Zhang
title	Upregulation of Mir342 in Diet-Induced Obesity Mouse and the Hypothalamic Appetite Control
title_short	Upregulation of Mir342 in Diet-Induced Obesity Mouse and the Hypothalamic Appetite Control
title_full	Upregulation of Mir342 in Diet-Induced Obesity Mouse and the Hypothalamic Appetite Control
title_fullStr	Upregulation of Mir342 in Diet-Induced Obesity Mouse and the Hypothalamic Appetite Control
title_full_unstemmed	Upregulation of Mir342 in Diet-Induced Obesity Mouse and the Hypothalamic Appetite Control
title_sort	upregulation of mir342 in diet-induced obesity mouse and the hypothalamic appetite control
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/d22838d67bcb410ca3e064ee7ca80218
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Upregulation of Mir342 in Diet-Induced Obesity Mouse and the Hypothalamic Appetite Control

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