Clinical Application of Metagenomic Next-Generation Sequencing in Patients with Hematologic Malignancies Suffering from Sepsis

Background: Sepsis remains a common but fatal complication among patients with immune suppression. We aimed to investigate the performance of metagenomic next-generation sequencing (mNGS) compared with standard microbiological diagnostics in patients with hematologic malignancies. Methods: We perfor...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wang-Da Liu, Ting-Yu Yen, Po-Yo Liu, Un-In Wu, Prerana Bhan, Yu-Chi Li, Chih-Hung Chi, Wang-Huei Sheng
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	blood culture neutropenia septic shock polymerase chain reaction Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d2307443acd14950a76a05eba0b5fc79
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d2307443acd14950a76a05eba0b5fc79
record_format	dspace
spelling	oai:doaj.org-article:d2307443acd14950a76a05eba0b5fc792021-11-25T18:25:00ZClinical Application of Metagenomic Next-Generation Sequencing in Patients with Hematologic Malignancies Suffering from Sepsis10.3390/microorganisms91123092076-2607https://doaj.org/article/d2307443acd14950a76a05eba0b5fc792021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2309https://doaj.org/toc/2076-2607Background: Sepsis remains a common but fatal complication among patients with immune suppression. We aimed to investigate the performance of metagenomic next-generation sequencing (mNGS) compared with standard microbiological diagnostics in patients with hematologic malignancies. Methods: We performed a prospective study from June 2019 to December 2019. Adult patients with hematologic malignancies and a clinical diagnosis of sepsis were enrolled. Conventional diagnostic methods included blood cultures, serum galactomannan for Aspergillus, cryptococcal antigen and cytomegalovirus (CMV) viral loads. Blood samples for mNGS were collected within 24 h after hypotension developed. Results: Of 24 patients enrolled, mNGS and conventional diagnostic methods (blood cultures, serology testing and virus RT-PCR) reached comparable positive results in 9 cases. Of ten patients, mNGS was able to identify additional pathogens compared with conventional methods; most of the pathogens were virus. Conclusion: Our results show that mNGS may serve as adjunctive diagnostic tool for the identification of pathogens of hematologic patients with clinically sepsis.Wang-Da LiuTing-Yu YenPo-Yo LiuUn-In WuPrerana BhanYu-Chi LiChih-Hung ChiWang-Huei ShengMDPI AGarticleblood cultureneutropeniaseptic shockpolymerase chain reactionBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2309, p 2309 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	blood culture neutropenia septic shock polymerase chain reaction Biology (General) QH301-705.5
spellingShingle	blood culture neutropenia septic shock polymerase chain reaction Biology (General) QH301-705.5 Wang-Da Liu Ting-Yu Yen Po-Yo Liu Un-In Wu Prerana Bhan Yu-Chi Li Chih-Hung Chi Wang-Huei Sheng Clinical Application of Metagenomic Next-Generation Sequencing in Patients with Hematologic Malignancies Suffering from Sepsis
description	Background: Sepsis remains a common but fatal complication among patients with immune suppression. We aimed to investigate the performance of metagenomic next-generation sequencing (mNGS) compared with standard microbiological diagnostics in patients with hematologic malignancies. Methods: We performed a prospective study from June 2019 to December 2019. Adult patients with hematologic malignancies and a clinical diagnosis of sepsis were enrolled. Conventional diagnostic methods included blood cultures, serum galactomannan for Aspergillus, cryptococcal antigen and cytomegalovirus (CMV) viral loads. Blood samples for mNGS were collected within 24 h after hypotension developed. Results: Of 24 patients enrolled, mNGS and conventional diagnostic methods (blood cultures, serology testing and virus RT-PCR) reached comparable positive results in 9 cases. Of ten patients, mNGS was able to identify additional pathogens compared with conventional methods; most of the pathogens were virus. Conclusion: Our results show that mNGS may serve as adjunctive diagnostic tool for the identification of pathogens of hematologic patients with clinically sepsis.
format	article
author	Wang-Da Liu Ting-Yu Yen Po-Yo Liu Un-In Wu Prerana Bhan Yu-Chi Li Chih-Hung Chi Wang-Huei Sheng
author_facet	Wang-Da Liu Ting-Yu Yen Po-Yo Liu Un-In Wu Prerana Bhan Yu-Chi Li Chih-Hung Chi Wang-Huei Sheng
author_sort	Wang-Da Liu
title	Clinical Application of Metagenomic Next-Generation Sequencing in Patients with Hematologic Malignancies Suffering from Sepsis
title_short	Clinical Application of Metagenomic Next-Generation Sequencing in Patients with Hematologic Malignancies Suffering from Sepsis
title_full	Clinical Application of Metagenomic Next-Generation Sequencing in Patients with Hematologic Malignancies Suffering from Sepsis
title_fullStr	Clinical Application of Metagenomic Next-Generation Sequencing in Patients with Hematologic Malignancies Suffering from Sepsis
title_full_unstemmed	Clinical Application of Metagenomic Next-Generation Sequencing in Patients with Hematologic Malignancies Suffering from Sepsis
title_sort	clinical application of metagenomic next-generation sequencing in patients with hematologic malignancies suffering from sepsis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d2307443acd14950a76a05eba0b5fc79
work_keys_str_mv	AT wangdaliu clinicalapplicationofmetagenomicnextgenerationsequencinginpatientswithhematologicmalignanciessufferingfromsepsis AT tingyuyen clinicalapplicationofmetagenomicnextgenerationsequencinginpatientswithhematologicmalignanciessufferingfromsepsis AT poyoliu clinicalapplicationofmetagenomicnextgenerationsequencinginpatientswithhematologicmalignanciessufferingfromsepsis AT uninwu clinicalapplicationofmetagenomicnextgenerationsequencinginpatientswithhematologicmalignanciessufferingfromsepsis AT preranabhan clinicalapplicationofmetagenomicnextgenerationsequencinginpatientswithhematologicmalignanciessufferingfromsepsis AT yuchili clinicalapplicationofmetagenomicnextgenerationsequencinginpatientswithhematologicmalignanciessufferingfromsepsis AT chihhungchi clinicalapplicationofmetagenomicnextgenerationsequencinginpatientswithhematologicmalignanciessufferingfromsepsis AT wanghueisheng clinicalapplicationofmetagenomicnextgenerationsequencinginpatientswithhematologicmalignanciessufferingfromsepsis
_version_	1718411226381287424

Clinical Application of Metagenomic Next-Generation Sequencing in Patients with Hematologic Malignancies Suffering from Sepsis

Ejemplares similares