A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection

Isothermal DNA amplification techniques are useful for diagnostic applications in place of traditional PCR. Here the authors describe CRISDA, which combines CRISPR–Cas9 with strand displacement amplification and exhibits attomolar sensitivity and single-nucleotide specificity in DNA detection.

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Auteurs principaux:	Wenhua Zhou, Li Hu, Liming Ying, Zhen Zhao, Paul K. Chu, Xue-Feng Yu
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2018
Sujets:	Science Q
Accès en ligne:	https://doaj.org/article/d237beec5d754883864eedecfd2f71be
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spelling	oai:doaj.org-article:d237beec5d754883864eedecfd2f71be2021-12-02T15:34:23ZA CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection10.1038/s41467-018-07324-52041-1723https://doaj.org/article/d237beec5d754883864eedecfd2f71be2018-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-07324-5https://doaj.org/toc/2041-1723Isothermal DNA amplification techniques are useful for diagnostic applications in place of traditional PCR. Here the authors describe CRISDA, which combines CRISPR–Cas9 with strand displacement amplification and exhibits attomolar sensitivity and single-nucleotide specificity in DNA detection.Wenhua ZhouLi HuLiming YingZhen ZhaoPaul K. ChuXue-Feng YuNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Wenhua Zhou Li Hu Liming Ying Zhen Zhao Paul K. Chu Xue-Feng Yu A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection
description	Isothermal DNA amplification techniques are useful for diagnostic applications in place of traditional PCR. Here the authors describe CRISDA, which combines CRISPR–Cas9 with strand displacement amplification and exhibits attomolar sensitivity and single-nucleotide specificity in DNA detection.
format	article
author	Wenhua Zhou Li Hu Liming Ying Zhen Zhao Paul K. Chu Xue-Feng Yu
author_facet	Wenhua Zhou Li Hu Liming Ying Zhen Zhao Paul K. Chu Xue-Feng Yu
author_sort	Wenhua Zhou
title	A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection
title_short	A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection
title_full	A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection
title_fullStr	A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection
title_full_unstemmed	A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection
title_sort	crispr–cas9-triggered strand displacement amplification method for ultrasensitive dna detection
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/d237beec5d754883864eedecfd2f71be
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A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection

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