A series of rat segmental forelimb ectopic implantation models

Abstract Temporary ectopic implantation has been performed in clinical practice to salvage devascularized amputated tissues for delayed replantation purpose. In this study, we established a series of segmental forelimb ectopic implantation models in rats, including forelimb, forearm, forepaw, digit,...

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Autores principales:	Xianyu Zhou, Xusong Luo, Bowen Gao, Fei Liu, Chuan Gu, Qingxiong Yu, Qingfeng Li, Hainan Zhu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d2452326d0fc4d81a42444ee260c9f95
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spelling	oai:doaj.org-article:d2452326d0fc4d81a42444ee260c9f952021-12-02T15:06:17ZA series of rat segmental forelimb ectopic implantation models10.1038/s41598-017-01342-x2045-2322https://doaj.org/article/d2452326d0fc4d81a42444ee260c9f952017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01342-xhttps://doaj.org/toc/2045-2322Abstract Temporary ectopic implantation has been performed in clinical practice to salvage devascularized amputated tissues for delayed replantation purpose. In this study, we established a series of segmental forelimb ectopic implantation models in rats, including forelimb, forearm, forepaw, digit, and double forelimbs, to mimic the clinical context. Time of amputated limbs harvesting in donors and ectopic implantation process in recipients were recorded. Survival time and mortalities of recipients were also recorded. Sixty days after ectopic implantation, a full-field laser perfusion imager (FLPI) was used to detect the blood flow of amputated limbs and micro-CT imaging was used to examine bone morphological changes. Histological sections of amputated limbs were stained with hematoxylin and eosin to evaluate pathological changes. Implanted amputated limbs in all models achieved long term survival and there were no obvious morphological and histological changes were found according to results of micro-CT and histology study. Thus, a series of rat segmental forelimb temporary ectopic implantation models have been well established. To our knowledge, this is the first rodent animal model related to forelimb temporary ectopic implantation. These models might facilitate further research related to salvage, reconstruction and better aesthetic and functional outcome of upper extremity/digit in temporary ectopic implantation scenario.Xianyu ZhouXusong LuoBowen GaoFei LiuChuan GuQingxiong YuQingfeng LiHainan ZhuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xianyu Zhou Xusong Luo Bowen Gao Fei Liu Chuan Gu Qingxiong Yu Qingfeng Li Hainan Zhu A series of rat segmental forelimb ectopic implantation models
description	Abstract Temporary ectopic implantation has been performed in clinical practice to salvage devascularized amputated tissues for delayed replantation purpose. In this study, we established a series of segmental forelimb ectopic implantation models in rats, including forelimb, forearm, forepaw, digit, and double forelimbs, to mimic the clinical context. Time of amputated limbs harvesting in donors and ectopic implantation process in recipients were recorded. Survival time and mortalities of recipients were also recorded. Sixty days after ectopic implantation, a full-field laser perfusion imager (FLPI) was used to detect the blood flow of amputated limbs and micro-CT imaging was used to examine bone morphological changes. Histological sections of amputated limbs were stained with hematoxylin and eosin to evaluate pathological changes. Implanted amputated limbs in all models achieved long term survival and there were no obvious morphological and histological changes were found according to results of micro-CT and histology study. Thus, a series of rat segmental forelimb temporary ectopic implantation models have been well established. To our knowledge, this is the first rodent animal model related to forelimb temporary ectopic implantation. These models might facilitate further research related to salvage, reconstruction and better aesthetic and functional outcome of upper extremity/digit in temporary ectopic implantation scenario.
format	article
author	Xianyu Zhou Xusong Luo Bowen Gao Fei Liu Chuan Gu Qingxiong Yu Qingfeng Li Hainan Zhu
author_facet	Xianyu Zhou Xusong Luo Bowen Gao Fei Liu Chuan Gu Qingxiong Yu Qingfeng Li Hainan Zhu
author_sort	Xianyu Zhou
title	A series of rat segmental forelimb ectopic implantation models
title_short	A series of rat segmental forelimb ectopic implantation models
title_full	A series of rat segmental forelimb ectopic implantation models
title_fullStr	A series of rat segmental forelimb ectopic implantation models
title_full_unstemmed	A series of rat segmental forelimb ectopic implantation models
title_sort	series of rat segmental forelimb ectopic implantation models
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d2452326d0fc4d81a42444ee260c9f95
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A series of rat segmental forelimb ectopic implantation models

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