CRISPR/Cas9-Mediated SlMYBS2 Mutagenesis Reduces Tomato Resistance to Phytophthora infestans

Phytophthora infestans (P. infestans) recently caused epidemics of tomato late blight. Our study aimed to identify the function of the SlMYBS2 gene in response to tomato late blight. To further investigate the function of SlMYBS2</...

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Autores principales:	Chunxin Liu, Yiyao Zhang, Yinxiao Tan, Tingting Zhao, Xiangyang Xu, Huanhuan Yang, Jingfu Li
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Solanum lycopersicum</i> <i>SlMYBS2</i> gene expression disease resistance <i>P. infestans</i> Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/d253a40a58f046659559eb57f3c11722
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spelling	oai:doaj.org-article:d253a40a58f046659559eb57f3c117222021-11-11T16:53:38ZCRISPR/Cas9-Mediated <i>SlMYBS2</i> Mutagenesis Reduces Tomato Resistance to <i>Phytophthora infestans</i>10.3390/ijms2221114231422-00671661-6596https://doaj.org/article/d253a40a58f046659559eb57f3c117222021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11423https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067<i>Phytophthora infestans</i> (<i>P. infestans</i>) recently caused epidemics of tomato late blight. Our study aimed to identify the function of the <i>SlMYBS2</i> gene in response to tomato late blight. To further investigate the function of <i>SlMYBS2</i> in tomato resistance to <i>P. infestans</i>, we studied the effects of <i>SlMYBS2</i> gene knock out. The <i>SlMYBS2</i> gene was knocked out by CRISPR-Cas9, and the resulting plants (<i>SlMYBS2</i> gene knockout, <i>slmybs2-c</i>) showed reduced resistance to <i>P. infestans</i>, accompanied by increases in the number of necrotic cells, lesion sizes, and disease index. Furthermore, after <i>P. infestans</i> infection, the expression levels of pathogenesis-related (<i>PR</i>) genes in <i>slmybs2-c</i> plants were significantly lower than those in wild-type (AC) plants, while the number of necrotic cells and the accumulation of reactive oxygen species (ROS) were higher than those in wild-type plants. Taken together, these results indicate that <i>SlMYBS2</i> acts as a positive regulator of tomato resistance to <i>P. infestans</i> infection by regulating the ROS level and the expression level of <i>PR</i> genes.Chunxin LiuYiyao ZhangYinxiao TanTingting ZhaoXiangyang XuHuanhuan YangJingfu LiMDPI AGarticle<i>Solanum lycopersicum</i><i>SlMYBS2</i>gene expressiondisease resistance<i>P. infestans</i>Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11423, p 11423 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Solanum lycopersicum</i> <i>SlMYBS2</i> gene expression disease resistance <i>P. infestans</i> Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Solanum lycopersicum</i> <i>SlMYBS2</i> gene expression disease resistance <i>P. infestans</i> Biology (General) QH301-705.5 Chemistry QD1-999 Chunxin Liu Yiyao Zhang Yinxiao Tan Tingting Zhao Xiangyang Xu Huanhuan Yang Jingfu Li CRISPR/Cas9-Mediated <i>SlMYBS2</i> Mutagenesis Reduces Tomato Resistance to <i>Phytophthora infestans</i>
description	<i>Phytophthora infestans</i> (<i>P. infestans</i>) recently caused epidemics of tomato late blight. Our study aimed to identify the function of the <i>SlMYBS2</i> gene in response to tomato late blight. To further investigate the function of <i>SlMYBS2</i> in tomato resistance to <i>P. infestans</i>, we studied the effects of <i>SlMYBS2</i> gene knock out. The <i>SlMYBS2</i> gene was knocked out by CRISPR-Cas9, and the resulting plants (<i>SlMYBS2</i> gene knockout, <i>slmybs2-c</i>) showed reduced resistance to <i>P. infestans</i>, accompanied by increases in the number of necrotic cells, lesion sizes, and disease index. Furthermore, after <i>P. infestans</i> infection, the expression levels of pathogenesis-related (<i>PR</i>) genes in <i>slmybs2-c</i> plants were significantly lower than those in wild-type (AC) plants, while the number of necrotic cells and the accumulation of reactive oxygen species (ROS) were higher than those in wild-type plants. Taken together, these results indicate that <i>SlMYBS2</i> acts as a positive regulator of tomato resistance to <i>P. infestans</i> infection by regulating the ROS level and the expression level of <i>PR</i> genes.
format	article
author	Chunxin Liu Yiyao Zhang Yinxiao Tan Tingting Zhao Xiangyang Xu Huanhuan Yang Jingfu Li
author_facet	Chunxin Liu Yiyao Zhang Yinxiao Tan Tingting Zhao Xiangyang Xu Huanhuan Yang Jingfu Li
author_sort	Chunxin Liu
title	CRISPR/Cas9-Mediated <i>SlMYBS2</i> Mutagenesis Reduces Tomato Resistance to <i>Phytophthora infestans</i>
title_short	CRISPR/Cas9-Mediated <i>SlMYBS2</i> Mutagenesis Reduces Tomato Resistance to <i>Phytophthora infestans</i>
title_full	CRISPR/Cas9-Mediated <i>SlMYBS2</i> Mutagenesis Reduces Tomato Resistance to <i>Phytophthora infestans</i>
title_fullStr	CRISPR/Cas9-Mediated <i>SlMYBS2</i> Mutagenesis Reduces Tomato Resistance to <i>Phytophthora infestans</i>
title_full_unstemmed	CRISPR/Cas9-Mediated <i>SlMYBS2</i> Mutagenesis Reduces Tomato Resistance to <i>Phytophthora infestans</i>
title_sort	crispr/cas9-mediated <i>slmybs2</i> mutagenesis reduces tomato resistance to <i>phytophthora infestans</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d253a40a58f046659559eb57f3c11722
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CRISPR/Cas9-Mediated <i>SlMYBS2</i> Mutagenesis Reduces Tomato Resistance to <i>Phytophthora infestans</i>

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